Recombinant Anti-IgE antibody [RM122] (ab195580)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM122] to IgE
- Suitable for: Sandwich ELISA
- Reacts with: Human
Overview
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Product name
Anti-IgE antibody [RM122]
See all IgE primary antibodies -
Description
Rabbit monoclonal [RM122] to IgE -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human IgE aa 1 to the C-terminus. IgE purified from Human myeloma plasma.
Database link: P01854
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab195580 was purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM122 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab195580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Sandwich ELISA |
Use at an assay dependent concentration.
10 ng/well – 100 ng/well (for Capture); 0.01 µg/mL – 0.1 µg/mL (for Detection) |
Notes |
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Sandwich ELISA
Use at an assay dependent concentration. 10 ng/well – 100 ng/well (for Capture); 0.01 µg/mL – 0.1 µg/mL (for Detection) |
Target
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Relevance
IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 3497 Human
- Omim: 147180 Human
- SwissProt: P01854 Human
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Alternative names
- Igh2 antibody
- IGHE antibody
- IGHEP1 antibody
- Immunoglobulin heavy constant epsilon antibody
Images
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Sandwich ELISA using ab195580 as the capture antibody (25 ng/well), and biotinylated anti-Human light chains ( κ+ λ) antibody clone RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab195580 has not yet been referenced specifically in any publications.