Recombinant

Recombinant Anti-IgE antibody [RM122] (ab195580)

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Overview

  • Product name

    Anti-IgE antibody [RM122]
    See all IgE primary antibodies

  • Description

    Rabbit monoclonal [RM122] to IgE

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Sandwich ELISA, ICC, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (purified) corresponding to Human IgE aa 1-428. IgE purified from Human myeloma plasma.
    Sequence:

    ASTQSPSVFPLTRCCKNIPSNATSVTLGCLATGYFPEPVMVTWDTGSLNG TTMTLPATTLTLSGHYATISLLTVSGAWAKQMFTCRVAHTPSSTDWVDNK TFSVCSRDFTPPTVKILQSSCDGGGHFPPTIQLLCLVSGYTPGTINITWL EDGQVMDVDLSTASTTQEGELASTQSELTLSQKHWLSDRTYTCQVTYQGH TFEDSTKKCADSNPRGVSAYLSRPSPFDLFIRKSPTITCLVVDLAPSKGT VNLTWSRASGKPVNHSTRKEEKQRNGTLTVTSTLPVGTRDWIEGETYQCR VTHPHLPRALMRSTTKTSGPRAAPEVYAFATPEWPGSRDKRTLACLIQNF MPEDISVQWLHNEVQLPDARHSTTQPRKTKGSGFFVFSRLEVTRAEWEQK DEFICRAVHEAASPSQTVQR AVSVNPGK


    Database link: P01854

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 48% PBS, 50% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    ab195580 was purified from an animal origin–free culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    RM122

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab195580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 2 µg/ml.
Sandwich ELISA Use at an assay dependent concentration.

10 ng/well – 100 ng/well (for Capture); 0.01 µg/mL – 0.1 µg/mL (for Detection)
ICC Use a concentration of 0.5 - 2 µg/ml.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.

  • Cellular localization

    Cell Membrane

  • Database links

    • Alternative names

      • Igh2 antibody
      • IGHE antibody
      • IGHEP1 antibody
      • Immunoglobulin heavy constant epsilon antibody

    Images

    • Sandwich ELISA - Anti-IgE antibody [RM122] (ab195580)
      Sandwich ELISA - Anti-IgE antibody [RM122] (ab195580)

      Sandwich ELISA using ab195580 as the capture antibody (25 ng/well), and biotinylated anti-Human light chains ( κ+ λ) antibody clone RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

    References

    ab195580 has not yet been referenced specifically in any publications.

    Publishing research using ab195580? Please let us know so that we can cite the reference in this datasheet.

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