Recombinant

Recombinant Anti-IgE antibody [RM122] (ab195580)

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Sandwich ELISA - Anti-IgE antibody [RM122] (ab195580)
  • Anti-IgE antibody [RM122] (ab195580)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RM122] to IgE
  • Suitable for: Sandwich ELISA
  • Reacts with: Human

Overview

  • Product name

    Anti-IgE antibody [RM122]
    See all IgE primary antibodies

  • Description

    Rabbit monoclonal [RM122] to IgE

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (purified) corresponding to Human IgE aa 1 to the C-terminus. IgE purified from Human myeloma plasma.
    Database link: P01854

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 48% PBS, 50% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    ab195580 was purified from an animal origin–free culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    RM122

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab195580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
Use at an assay dependent concentration.

10 ng/well – 100 ng/well (for Capture); 0.01 µg/mL – 0.1 µg/mL (for Detection)
Notes
Sandwich ELISA
Use at an assay dependent concentration.

10 ng/well – 100 ng/well (for Capture); 0.01 µg/mL – 0.1 µg/mL (for Detection)

Target

  • Relevance

    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.

  • Cellular localization

    Cell Membrane

  • Database links

    • Alternative names

      • Igh2 antibody
      • IGHE antibody
      • IGHEP1 antibody
      • Immunoglobulin heavy constant epsilon antibody

    Images

    • Sandwich ELISA - Anti-IgE antibody [RM122] (ab195580)
      Sandwich ELISA - Anti-IgE antibody [RM122] (ab195580)

      Sandwich ELISA using ab195580 as the capture antibody (25 ng/well), and biotinylated anti-Human light chains ( κ+ λ) antibody clone RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

    • Anti-IgE antibody [RM122] (ab195580)
      Anti-IgE antibody [RM122] (ab195580)

    References (0)

    Publishing research using ab195580? Please let us know so that we can cite the reference in this datasheet.

    ab195580 has not yet been referenced specifically in any publications.

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