Overview

  • Product name
    Anti-IgE antibody [XTE4]
    See all IgE primary antibodies
  • Description
    Mouse monoclonal [XTE4] to IgE
  • Host species
    Mouse
  • Specificity
    Epsilon chain specific
  • Tested applications
    Suitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human IgE. IgE from myelomatous human serum

  • General notes

    Concentration varies from lot to lot and can be provided on request.

     

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.50
    Preservative: 0.05% Sodium azide
    Constituents: 0.121% Tris, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    XTE4
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1e+006. Can be used for the detection of total IgE level and IgE antibodies in ELISA.
WB Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution. PubMed: 21307287

Target

  • Relevance
    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • Igh2 antibody
    • IGHE antibody
    • IGHEP1 antibody
    • Immunoglobulin heavy constant epsilon antibody

References

This product has been referenced in:
  • Palaniyandi S  et al. CD23-Dependent Transcytosis of IgE and Immune Complex across the Polarized Human Respiratory Epithelial Cells. J Immunol 186:3484-96 (2011). WB, ICC/IF, ELISA ; Human . Read more (PubMed: 21307287) »
See 1 Publication for this product

Customer reviews and Q&As

Question

Dear Abcam tech support,
Please advise on the following enquiry posted by our customer:
I'm preparing some western blot experiments using the human serum or plasma hypersensitive to the allergen of interest to a recombinant
allergen I've made. The steps for the western blot that I have
planned are as follows:
(1) Run SDS-PAGE of the recombinant allergen protein
(2) Transfer the allergen protein from the SDS-PAGE gel to a nitrocellulose membrane
(3) Incubate membrane with human serum/plasma known to be hypersensitive to the allergen (human IgE binds to allergen on the
membrane)
(4) Incubate membrane with mouse/rat/rabbit anti-human IgE antibody which will bind to the human IgE
(5) Incubate membrane with anti-mouse/rate/rabbit anti-IgG conjugated with HRP (secondary antibody)
(6) Visualise result with ECL.
My questions are as follows:
(a) would the mouse monoclonal to human IgE (ab7382) be appropriate?
If so, could you let me know the price for this antibody?
(b) do you have a secondary antibody conjucated to HRP that I could use for step 5? If so, could you let me know the price as well?
The reason why I'm doing steps (4) and (5) instead of just step (4) with an anti-IgE conjugated to HRP is that I've been told that as IgE is usually in very low abundance in human serum/plasma, thus I would probably be unable to detect the IgE directly without doing step (5).
But I'm not sure if there are sensitive antibodies that could do away with step (5). I have read some papers where people have skipped step
(5) for Western Blot. Do you have a product that is sensitive enough so that I can skip step 5 altogether? If so, could you let me have the quotation as well?
Please advise,
In the meantime, have a nice day!
Kind regards,

Read More
Answer

Thank you for contacting us and sorry for the delay in getting back to you.

In answer to your questions:

1. Yes, I believe the antibody ab7382 should be suitable for the experiment you have described, however, as we have not performed a Western blot in this way (directly binding the human IgE to the membrane), we would not know exactly how the antibody would perform. It may be that you would need to perform some optimisation of the protocol in order to obtain the best results.

2. The goat anti-mouse IgG1 (https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-HRP-pre-adsorbed-ab98693.html) should be suitable for the experiment you are planning. Sapphire will have to inform you of what the price of this will be.

In answer to your question of if the directly conjugated, ab7294 could be used instead of using the primary ab7382 followed by the secondary antibody, I would suggest this would be possible but as you have said the signal would be weaker, so if the target is not abundant then using a secondary antibody would be beneficial. Additionally, as we have only used ab7294 in ELISAs as yet, we would not be able to know how it would perform in a Western blot. I would therefore suggest using ab7382 with a secondary antibody may be more appropriate.

I hope this information has been of some help. If you have any further questions, please do not hesitate to ask.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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