Anti-IGF1 antibody (ab106836)
Key features and details
- Goat polyclonal to IGF1
- Suitable for: WB, ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-IGF1 antibody
See all IGF1 primary antibodies -
Description
Goat polyclonal to IGF1 -
Host species
Goat -
Tested applications
Suitable for: WB, ICC, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig -
Immunogen
Peptide with sequence
C-RSVRAQRHTD
, from the internal region of human IGF1 sequence according to NP_001104753.1; NP_001104754.1; NP_001104755.1; NP_000609.1. -
Positive control
- ICC: Human HEK-293 cells; WB: Human uterus tissue lysate; IHC-P: Human spleen tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 22 kDa).
1 hour primary incubation is recommended for this product. |
ICC |
Use a concentration of 10 µg/ml.
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IHC-P |
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 22 kDa). 1 hour primary incubation is recommended for this product. |
ICC
Use a concentration of 10 µg/ml. |
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
Involvement in disease
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
Sequence similarities
Belongs to the insulin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 610255 Dog
- Entrez Gene: 3479 Human
- Entrez Gene: 16000 Mouse
- Entrez Gene: 24482 Rat
- Omim: 147440 Human
- SwissProt: P33712 Dog
- SwissProt: P01343 Human
- SwissProt: P05019 Human
see all -
Form
There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA. -
Alternative names
- IBP1 antibody
- IGF I antibody
- IGF IA antibody
see all
Images
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All lanes : Anti-IGF1 antibody (ab106836) at 0.5 µg/ml
Lane 1 : Human uterus tissue lysate in RIPA buffer
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 22 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour at room temperature. Detected by chemiluminescence.
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Immunocytochemistry analysis of paraformaldehyde fixed HEK-293 (human epithelial cell line from embryonic kidney) cells, permeabilized with 0.15% Triton. Primary incubation for 1 hour at 10 µg/mL followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab106836 has been referenced in 12 publications.
- Wu H et al. Engineered adipose-derived stem cells with IGF-1-modified mRNA ameliorates osteoarthritis development. Stem Cell Res Ther 13:19 (2022). PubMed: 35033199
- Choi YJ et al. Ameliorative effect of Abeliophyllum distichum Nakai on benign prostatic hyperplasia in vitro and in vivo. Nutr Res Pract 16:419-434 (2022). PubMed: 35919286
- Zeng N et al. Sexual dimorphic effects of igf1 deficiency on metabolism in zebrafish. Front Endocrinol (Lausanne) 13:879962 (2022). PubMed: 35966057
- Xia H et al. Yi-Qi-Jian-Pi-Xiao-Yu-Xie-Zhuo Formula Improves Muscle Atrophy via Modulating the IGF-1/PI3K/Akt Signaling Pathway in 5/6 Nephrectomized Rats. Front Pharmacol 12:624303 (2021). PubMed: 33986663
- Ai D & Yu F LncRNA DNM3OS promotes proliferation and inhibits apoptosis through modulating IGF1 expression by sponging MiR-126 in CHON-001 cells. Diagn Pathol 14:106 (2019). PubMed: 31526393