Anti-IGF1 antibody (ab106836)

Goat polyclonal IGF1 antibody. Validated in WB, ICC/IF and tested in Human, Zebrafish. Cited in 2 publication(s). Independently reviewed in 1 review(s). Immunogen corresponding to synthetic peptide.

Overview

  • Product name

  • Description

    Goat polyclonal to IGF1
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human, Zebrafish
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig
  • Immunogen

    Peptide with sequence

    C-RSVRAQRHTD

    , from the internal region of human IGF1 sequence according to NP_001104753.1; NP_001104754.1; NP_001104755.1; NP_000609.1.

  • Positive control

    • Human uterus lysate. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HeLa

Properties

Applications

Our Abpromise guarantee covers the use of ab106836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 22 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.
  • Involvement in disease

    Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
  • Sequence similarities

    Belongs to the insulin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Form

    There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA.
  • Alternative names

    • IBP1 antibody
    • IGF I antibody
    • IGF IA antibody
    • IGF IB antibody
    • IGF-I antibody
    • Igf1 antibody
    • IGF1_HUMAN antibody
    • IGF1A antibody
    • IGFI antibody
    • IGFIA antibody
    • Insulin like growth factor 1 (somatomedin C) antibody
    • Insulin like growth factor 1 antibody
    • Insulin like growth factor IA antibody
    • Insulin like growth factor IB antibody
    • Insulin-like growth factor I antibody
    • Mechano growth factor antibody
    • MGF antibody
    • OTTHUMP00000195080 antibody
    • OTTHUMP00000195081 antibody
    • OTTHUMP00000195082 antibody
    • OTTHUMP00000195083 antibody
    • OTTHUMP00000195084 antibody
    • Somatomedia C antibody
    • Somatomedin C antibody
    • Somatomedin-C antibody
    see all

Images

  • Anti-IGF1 antibody (ab106836) at 0.5 µg/ml + Human uterus lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 22 kDa

  • ab106836 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab106836 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Hu T  et al. Electro-acupuncture-induced neuroprotection is associated with activation of the IGF-1/PI3K/Akt pathway following adjacent dorsal root ganglionectomies in rats. Int J Mol Med 43:807-820 (2019). Read more (PubMed: 30569108) »
  • Chen W  et al. Astrocytic Insulin-Like Growth Factor-1 Protects Neurons Against Excitotoxicity. Front Cell Neurosci 13:298 (2019). Read more (PubMed: 31338023) »
See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12%)
Sample
Zebrafish Tissue lysate - whole (muslce (igf1 overexpression in transgenic fish mus)
Specification
muslce (igf1 overexpression in transgenic fish mus
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C

Dr. Dongliang Li

Verified customer

Submitted Nov 28 2013

Answer

Merci de votre intérêt pour les anti-IGF1 de chez Abcam.

Aucun des anticorps anti-IGF1 Abcam n’a encore été testé à la fois chez l'homme, la souris et le rat et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires. Cependant, si vous souhaitez tester ab106836 par exemple (www.abcam.com/ab106836), il me sera possible de vous offrir une remise. Après soumission d’une Abreview avec vos résultats, un avoir de la valeur de l'anticorps testé vous sera offert.

Etapes à suivre :

1. Nous confirmer que vous souhaitez tester ab106836 chez le rat et/ou la souris afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de ab106836

2. Acheter ab106836 par téléphone, fax ou internet (www.abcam.com)

3. Le tester chez le rat et/ou la souris

4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews

5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un autre anticorps primaire.
Même si ab106836 ne pas fonctionne pas chez les espèces testées, après soumission de votre Abreview, vous recevrez quand même un avoir de la valeur de l'anticorps testé.

N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle.

Termes et Conditions : www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting us. We can recomend using Tris triton or RIPA buffer for lysates preparation. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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