Anti-IGF1 antibody (ab223567)
Key features and details
- Rabbit polyclonal to IGF1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-IGF1 antibody
See all IGF1 primary antibodies -
Description
Rabbit polyclonal to IGF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human IGF1. From mid-protein of human IGF1.
Database link: P05019 -
Positive control
- WB: Mouse liver lysate. ICC/IF: RKO (human colon carcinoma cell line) cells. IHC-P: Human liver.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223567 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000.
|
ICC/IF |
1/100.
|
|
IHC-P |
1/50.
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Notes |
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WB
1/1000. |
ICC/IF
1/100. |
IHC-P
1/50. |
Target
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Function
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
Involvement in disease
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
Sequence similarities
Belongs to the insulin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3479 Human
- Entrez Gene: 16000 Mouse
- Omim: 147440 Human
- SwissProt: P01343 Human
- SwissProt: P05019 Human
- SwissProt: P05017 Mouse
- Unigene: 160562 Human
- Unigene: 268521 Mouse
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Form
There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA. -
Alternative names
- IBP1 antibody
- IGF I antibody
- IGF IA antibody
see all
Images
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Anti-IGF1 antibody (ab223567) at 1/1000 dilution + mouse liver lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG HRP at 1/2000 dilution
Developed using the ECL technique.
Observed band size: 22 kDa why is the actual band size different from the predicted?
Exposure time: 6 minutesBlocking: 5% skimmed milk in 1X TBST.
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4% formaldehyde fixed RKO (human colon carcinoma cell line) cells stained for IGF1 (green) using ab223567 at a 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit 488 at a 1/100 dilution. Phalloidin Texas Red F-Actin 1/1000 (red) and DAPI 1/5000 (blue) were used as counterstains.
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Immunohistochemical analysis of paraffin-embedded human liver tissue labelling IGF1 with ab223567 at 1/50 dilution, using Goat anti Rabbit (HRP) as the secondary antibody. Membrane and cytoplasmic staining on human liver tissue is observed. Counter stained with Hematoxylin. HRP-DAB Detection.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab223567 has been referenced in 3 publications.
- Zhang Z et al. Icariin regulates stem cell migration for endogenous repair of intervertebral disc degeneration by increasing the expression of chemotactic cytokines. BMC Complement Med Ther 22:63 (2022). PubMed: 35272637
- Stojakovic A et al. Partial inhibition of mitochondrial complex I ameliorates Alzheimer's disease pathology and cognition in APP/PS1 female mice. Commun Biol 4:61 (2021). PubMed: 33420340
- Shen B et al. Long non-coding RNA ZFAS1 exerts a protective role to alleviate oxygen and glucose deprivation-mediated injury in ischemic stroke cell model through targeting miR-186-5p/MCL1 axis. Cytotechnology 73:605-617 (2021). PubMed: 34349350