Overview

  • Product name

  • Description

    Rabbit polyclonal to IGF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human IGF1. From mid-protein of human IGF1.
    Database link: P05019

  • Positive control

    • WB: Mouse liver lysate. ICC/IF: RKO (human colon carcinoma cell line) cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab223567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
ICC/IF 1/100.

Target

  • Function

    The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.
  • Involvement in disease

    Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
  • Sequence similarities

    Belongs to the insulin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Form

    There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA.
  • Alternative names

    • IBP1 antibody
    • IGF I antibody
    • IGF IA antibody
    • IGF IB antibody
    • IGF-I antibody
    • Igf1 antibody
    • IGF1_HUMAN antibody
    • IGF1A antibody
    • IGFI antibody
    • IGFIA antibody
    • Insulin like growth factor 1 (somatomedin C) antibody
    • Insulin like growth factor 1 antibody
    • Insulin like growth factor IA antibody
    • Insulin like growth factor IB antibody
    • Insulin-like growth factor I antibody
    • Mechano growth factor antibody
    • MGF antibody
    • OTTHUMP00000195080 antibody
    • OTTHUMP00000195081 antibody
    • OTTHUMP00000195082 antibody
    • OTTHUMP00000195083 antibody
    • OTTHUMP00000195084 antibody
    • Somatomedia C antibody
    • Somatomedin C antibody
    • Somatomedin-C antibody
    see all

Images

  • Anti-IGF1 antibody (ab223567) at 1/1000 dilution + mouse liver lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG HRP at 1/2000 dilution

    Developed using the ECL technique.

    Observed band size: 22 kDa
    why is the actual band size different from the predicted?


    Exposure time: 6 minutes


    Blocking: 5% skimmed milk in 1X TBST.

  • 4% formaldehyde fixed RKO (human colon carcinoma cell line) cells stained for IGF1 (green) using ab223567 at a 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit 488 at a 1/100 dilution. Phalloidin Texas Red F-Actin 1/1000 (red) and DAPI 1/5000 (blue) were used as counterstains.

References

ab223567 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223567.
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