Overview

  • Product name
  • Description
    Rabbit polyclonal to IGF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC (PFA fixed)more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: a wide range of other species
  • Immunogen

    Synthetic human insulin-like growth factor 1 [Ac60-70] covalently attached onto a carrier protein.

  • Positive control
    • This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: Hek293.

Properties

Applications

Our Abpromise guarantee covers the use of ab40657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC (PFA fixed) 1/1000.

Target

  • Function
    The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.
  • Involvement in disease
    Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
  • Sequence similarities
    Belongs to the insulin family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Form
    There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA.
  • Alternative names
    • IBP1 antibody
    • IGF I antibody
    • IGF IA antibody
    • IGF IB antibody
    • IGF-I antibody
    • Igf1 antibody
    • IGF1_HUMAN antibody
    • IGF1A antibody
    • IGFI antibody
    • IGFIA antibody
    • Insulin like growth factor 1 (somatomedin C) antibody
    • Insulin like growth factor 1 antibody
    • Insulin like growth factor IA antibody
    • Insulin like growth factor IB antibody
    • Insulin-like growth factor I antibody
    • Mechano growth factor antibody
    • MGF antibody
    • OTTHUMP00000195080 antibody
    • OTTHUMP00000195081 antibody
    • OTTHUMP00000195082 antibody
    • OTTHUMP00000195083 antibody
    • OTTHUMP00000195084 antibody
    • Somatomedia C antibody
    • Somatomedin C antibody
    • Somatomedin-C antibody
    see all

Images

  • ab40657 staining IGF1 in murine prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed with paraformaldehyde and an antigen retrieval step was performed using citrate buffer. Samples were then blocked with 1% serum for 20 minutes at 25°C and then incubated with ab40657 at a 1/1000 dilution for 18 hours at 4°C. The secondary used was a biotin conjugated horse polyclonal used at a 1/50 dilution.

    See Abreview

  • ab40657 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab40657 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of rat colon tissue, staining IGF1 with ab40657.

    Tissue was fixed with formaldehyde and blocked with 1% blocking solution for 15 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/400 in BSA in TBS) for 1 hour. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Botusan IR  et al. Deficiency of liver-derived insulin-like growth factor-I (IGF-I) does not interfere with the skin wound healing rate. PLoS One 13:e0193084 (2018). Read more (PubMed: 29534073) »
  • Thomsen J  et al. PAPP-A proteolytic activity enhances IGF bioactivity in ascites from women with ovarian carcinoma. Oncotarget 6:32266-78 (2015). IHC . Read more (PubMed: 26336825) »

See all 4 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Diva
Permeabilization
No
Specification
Intestine
Blocking step
Rodent Block M as blocking agent for 30 minute(s) · Concentration: 0% · Temperature: R.T°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 10 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tooth)
Antigen retrieval step
None
Permeabilization
No
Specification
tooth
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 02 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Mouse Cell lysate - whole cell (MC3T3-E1 osteoblasts)
Specification
MC3T3-E1 osteoblasts
Treatment
Pre and post differentiation with osteogenic medium
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Benny Garfinkel

Verified customer

Submitted Aug 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TE pH 9.0
Sample
Mouse Tissue sections (Ovary)
Specification
Ovary
Permeabilization
No
Fixative
Paraformaldehyde
Username

Mr. Benny Garfinkel

Verified customer

Submitted Aug 12 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Colon)
Specification
Colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6 Citrate
Permeabilization
No
Blocking step
DAKO PB as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Nov 30 2012

Thank you for contacting Abcam regarding ab40657.


The sequence of mature IGF -1 (Ac-60-70) is as follows:Ac-YCAPLKPAKSA


I apologize for any confusion created. I have requested that our online datasheet be updated.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Prostate)
Specification
Prostate
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. Lucila Sackmann Sala

Verified customer

Submitted Apr 10 2012

Thank you for contacting us and for your Abreview.

I have linked the discount code to your Abreview. The code is now activated, you can place an order for a free antibody.

I hope this information is helpful to you. Please do not hes...

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DISCOUNT CODE: ******
Expiration date: JJ MM YYYY

I am very pleased to hear you would like to accept our offer and test ab40657 in Mouse (100% homology with the immunogen). This code will give you 1 freeprimary antibodybefore the expira...

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Thank you for your enquiry and your interest in our products. Native human IGF-1 is a 70 amino acid long peptide. We wanted to develop an antibody that bounds specifically to the -COOH terminus of the whole peptide, so we synthesized an 11-mer, i.e....

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