Overview

  • Product name
  • Description
    Rabbit polyclonal to IGF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC (PFA fixed)more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: a wide range of other species
  • Immunogen

    Synthetic human insulin-like growth factor 1 [Ac60-70] covalently attached onto a carrier protein.

  • Positive control
    • This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: Hek293.

Properties

Applications

Our Abpromise guarantee covers the use of ab40657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC (PFA fixed) 1/1000.

Target

  • Function
    The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.
  • Involvement in disease
    Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
  • Sequence similarities
    Belongs to the insulin family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Form
    There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA.
  • Alternative names
    • IBP1 antibody
    • IGF I antibody
    • IGF IA antibody
    • IGF IB antibody
    • IGF-I antibody
    • Igf1 antibody
    • IGF1_HUMAN antibody
    • IGF1A antibody
    • IGFI antibody
    • IGFIA antibody
    • Insulin like growth factor 1 (somatomedin C) antibody
    • Insulin like growth factor 1 antibody
    • Insulin like growth factor IA antibody
    • Insulin like growth factor IB antibody
    • Insulin-like growth factor I antibody
    • Mechano growth factor antibody
    • MGF antibody
    • OTTHUMP00000195080 antibody
    • OTTHUMP00000195081 antibody
    • OTTHUMP00000195082 antibody
    • OTTHUMP00000195083 antibody
    • OTTHUMP00000195084 antibody
    • Somatomedia C antibody
    • Somatomedin C antibody
    • Somatomedin-C antibody
    see all

Images

  • ab40657 staining IGF1 in murine prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed with paraformaldehyde and an antigen retrieval step was performed using citrate buffer. Samples were then blocked with 1% serum for 20 minutes at 25°C and then incubated with ab40657 at a 1/1000 dilution for 18 hours at 4°C. The secondary used was a biotin conjugated horse polyclonal used at a 1/50 dilution.

    See Abreview

  • ab40657 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab40657 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of rat colon tissue, staining IGF1 with ab40657.

    Tissue was fixed with formaldehyde and blocked with 1% blocking solution for 15 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/400 in BSA in TBS) for 1 hour. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Botusan IR  et al. Deficiency of liver-derived insulin-like growth factor-I (IGF-I) does not interfere with the skin wound healing rate. PLoS One 13:e0193084 (2018). Read more (PubMed: 29534073) »
  • Jin C  et al. Methyl donors dietary supplementation to gestating sows diet improves the growth rate of offspring and is associating with changes in expression and DNA methylation of insulin-like growth factor-1 gene. J Anim Physiol Anim Nutr (Berl) 102:1340-1350 (2018). Read more (PubMed: 29959805) »
See all 5 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Diva
Permeabilization
No
Specification
Intestine
Blocking step
Rodent Block M as blocking agent for 30 minute(s) · Concentration: 0% · Temperature: R.T°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 10 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tooth)
Antigen retrieval step
None
Permeabilization
No
Specification
tooth
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 02 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Mouse Cell lysate - whole cell (MC3T3-E1 osteoblasts)
Specification
MC3T3-E1 osteoblasts
Treatment
Pre and post differentiation with osteogenic medium
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Mr. Benny Garfinkel

Verified customer

Submitted Aug 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TE pH 9.0
Sample
Mouse Tissue sections (Ovary)
Specification
Ovary
Permeabilization
No
Fixative
Paraformaldehyde

Mr. Benny Garfinkel

Verified customer

Submitted Aug 12 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Colon)
Specification
Colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6 Citrate
Permeabilization
No
Blocking step
DAKO PB as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Nov 30 2012

Answer

Thank you for contacting Abcam regarding ab40657.


The sequence of mature IGF -1 (Ac-60-70) is as follows:Ac-YCAPLKPAKSA


I apologize for any confusion created. I have requested that our online datasheet be updated.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Prostate)
Specification
Prostate
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C

Dr. Lucila Sackmann Sala

Verified customer

Submitted Apr 10 2012

Answer

Thank you for contacting us and for your Abreview.

I have linked the discount code to your Abreview. The code is now activated, you can place an order for a free antibody.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

DISCOUNT CODE: ******
Expiration date: JJ MM YYYY

I am very pleased to hear you would like to accept our offer and test ab40657 in Mouse (100% homology with the immunogen). This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forMouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for your enquiry and your interest in our products. Native human IGF-1 is a 70 amino acid long peptide. We wanted to develop an antibody that bounds specifically to the -COOH terminus of the whole peptide, so we synthesized an 11-mer, i.e. residues #60-70 aa - to use as immunogen. Since we didn't want a free NH2-group at the amino terminal, we capped the amino terminus during synthesis by acetylating it with acetic anhydride which adds an acetyl group, i.e. the Ac moiety. Thus the resulting synthetic analogue of the carboxyl terminal region is Ac60-70. This synthetic peptide was coupled onto bovine thyroglobulin as carrier protein to elicit an antibody response since the immune system will not recognize small peptides, such as this 11-amino acid long analogue, as an antigen without it being conjugated onto a much larger protein. I hope this helps and if I can assist further, please do not hesitate to contact me.

Read More

1-10 of 11 Abreviews or Q&A

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