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Dear technical team, Our customer would like to purchase ab40657 and has inquiry about immnogen information. https://www.abcam.com/IGF1-antibody-ab40657.html It described as "Synthetic human insulin-like growth factor 1 [Ac60-70] covalently attached onto a carrier protein." 1. Why is this attached onto a carrier protein? The customer asked about the role of carrier protein and effect on antibody activity. 2. What is the meaning of "Ac" ([Ac60-70] )? Check them please. Thank you.
Asked on Aug 08 2011
Thank you for your enquiry and your interest in our products. Native human IGF-1 is a 70 amino acid long peptide. We wanted to develop an antibody that bounds specifically to the -COOH terminus of the whole peptide, so we synthesized an 11-mer, i.e. residues #60-70 aa - to use as immunogen. Since we didn't want a free NH2-group at the amino terminal, we capped the amino terminus during synthesis by acetylating it with acetic anhydride which adds an acetyl group, i.e. the Ac moiety. Thus the resulting synthetic analogue of the carboxyl terminal region is Ac60-70. This synthetic peptide was coupled onto bovine thyroglobulin as carrier protein to elicit an antibody response since the immune system will not recognize small peptides, such as this 11-amino acid long analogue, as an antigen without it being conjugated onto a much larger protein. I hope this helps and if I can assist further, please do not hesitate to contact me.
Answered on Aug 08 2011