Overview

  • Product name

  • Description

    Rabbit polyclonal to IGF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Neutralisingmore details
  • Species reactivity

    Reacts with: Mouse, Recombinant fragment
  • Immunogen

    Recombinant murine IGF1

Properties

Applications

Our Abpromise guarantee covers the use of ab63926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 17 kDa).
Neutralising Use a concentration of 2 - 4 µg/ml.

Target

  • Function

    The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.
  • Involvement in disease

    Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
  • Sequence similarities

    Belongs to the insulin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Form

    There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA.
  • Alternative names

    • IBP1 antibody
    • IGF I antibody
    • IGF IA antibody
    • IGF IB antibody
    • IGF-I antibody
    • Igf1 antibody
    • IGF1_HUMAN antibody
    • IGF1A antibody
    • IGFI antibody
    • IGFIA antibody
    • Insulin like growth factor 1 (somatomedin C) antibody
    • Insulin like growth factor 1 antibody
    • Insulin like growth factor IA antibody
    • Insulin like growth factor IB antibody
    • Insulin-like growth factor I antibody
    • Mechano growth factor antibody
    • MGF antibody
    • OTTHUMP00000195080 antibody
    • OTTHUMP00000195081 antibody
    • OTTHUMP00000195082 antibody
    • OTTHUMP00000195083 antibody
    • OTTHUMP00000195084 antibody
    • Somatomedia C antibody
    • Somatomedin C antibody
    • Somatomedin-C antibody
    see all

Images

  • All lanes : Anti-IGF1 antibody (ab63926) at 4 µg/ml

    Lane 1 : Recombinant
    murine IGF1 at 0.25 µg
    Lane 2 : Recombinant
    murine IGF1 at 1 µg

    Predicted band size: 17 kDa
    Observed band size: 9 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Durham E  et al. Thyroxine Exposure Effects on the Cranial Base. Calcif Tissue Int 101:300-311 (2017). Read more (PubMed: 28391432) »
  • Garcia LA  et al. 1,25(OH)2vitamin D3 stimulates myogenic differentiation by inhibiting cell proliferation and modulating the expression of promyogenic growth factors and myostatin in C2C12 skeletal muscle cells. Endocrinology 152:2976-86 (2011). WB ; Mouse . Read more (PubMed: 21673099) »
See all 3 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 16 2016

Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 16 2016

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15% Gel)
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 12 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15% Gel)
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 11 2014

Answer

The laboratory completed the blocking study for ab177467 (IGF2), and found that the blocking peptide did successfully block the 50 kDa band, indicating that this band is associated with the immunogen.

We found the 2 attached papers, which cite that a 50 kDa complex does exist for both IGF1 and IGF2 in serum, so these complexes may be present in your mouse skin as well.

From IGF1 paper:

“In serum, the majority (70–80%) of the IGFs exist in a 150-kDa complex comprised of one IGF molecule, IGFBP-3, and the acid labile subunit (ALS). A smaller proportion (∼20%) of the IGFs are associated with other serum IGFBPs within a 50-kDa complex, and less than 5% of the IGFs are found in the free form of 7.5 kDa.”

From IGF2 paper:

˜50kDa complex formed by IGF-II and IGFBP in the circulation.

Read More

Answer

ab63926 has not been tested with mouse skin samples. It seems in serum, IGF1 exists in a large polymeric complex (Refer to the introduction in the attached paper)
“In serum, the majority (70–80%) of the IGFs exist in a 150-kDa complex comprised of one IGF molecule, IGFBP-3, and the acid labile subunit (ALS). A smaller proportion (∼20%) of the IGFs are associated with other serum IGFBPs within a 50-kDa complex, and less than 5% of the IGFs are found in the free form of 7.5 kDa.”
Perhaps something similar could be happening in this sample.

Read More
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Organ, 293 cell, C2C12 cell)
Loading amount
25 µg
Specification
Organ, 293 cell, C2C12 cell
Gel Running Conditions
Reduced Denaturing (10% Tris-Tricine gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 19 2010

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