Anti-IGF1 antibody (ab9572)

Reviews (8)Q&A (19)References (95)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 antibody (ab9572)
  • Sandwich ELISA - Anti-IGF1 antibody (ab9572)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 antibody (ab9572)

Key features and details

  • Rabbit polyclonal to IGF1
  • Suitable for: ICC/IF, IHC-FoFr, WB, Neutralising, IHC-P, Sandwich ELISA
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-IGF1 antibody [EPR5098(2)] (ab133542)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-IGF1 antibody
    See all IGF1 primary antibodies

  • Description

    Rabbit polyclonal to IGF1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-FoFr, WB, Neutralising, IHC-P, Sandwich ELISAmore details

  • Species reactivity

    Reacts with: Mouse, Human, Recombinant fragment

  • Immunogen

    Recombinant full length protein corresponding to Human IGF1. Highly pure (>98%) recombinant hIGF-1 (human Insulin Like Growth Factor-1).
    Database link: P05019

  • Positive control

    • IHC-P: Human pancreas and liver tissues.

  • General notes

    Although some customers have been successful in IHC we no longer batch test in this application. For an IHC validated antibody please see ab263903

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (2)
Use at an assay dependent concentration.
IHC-FoFr (1)
Use at an assay dependent concentration. PubMed: 28754163
WB (2)
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.

To detect hIGF-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Neutralising
Use at an assay dependent concentration.

To yield one-half maximal inhibition of the biological activity of hIGF-1 (5.0 ng/ml), a concentration of 0.67 - 1.0 ug/ml of this antibody is required.
IHC-P (1)
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Biotin Rabbit polyclonal to IGF1 (ab83137). Allows for the detection of at least 0.2 - 0.4 ng/well of recombinant IGF1.
Notes
ICC/IF
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 28754163
WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.

To detect hIGF-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Neutralising
Use at an assay dependent concentration.

To yield one-half maximal inhibition of the biological activity of hIGF-1 (5.0 ng/ml), a concentration of 0.67 - 1.0 ug/ml of this antibody is required.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Biotin Rabbit polyclonal to IGF1 (ab83137). Allows for the detection of at least 0.2 - 0.4 ng/well of recombinant IGF1.

Target

  • Function

    The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.

  • Involvement in disease

    Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.

  • Sequence similarities

    Belongs to the insulin family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Form

      There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA.

    • Alternative names

      • IBP1 antibody
      • IGF I antibody
      • IGF IA antibody
      • IGF IB antibody
      • IGF-I antibody
      • Igf1 antibody
      • IGF1_HUMAN antibody
      • IGF1A antibody
      • IGFI antibody
      • IGFIA antibody
      • Insulin like growth factor 1 (somatomedin C) antibody
      • Insulin like growth factor 1 antibody
      • Insulin like growth factor IA antibody
      • Insulin like growth factor IB antibody
      • Insulin-like growth factor I antibody
      • Mechano growth factor antibody
      • MGF antibody
      • OTTHUMP00000195080 antibody
      • OTTHUMP00000195081 antibody
      • OTTHUMP00000195082 antibody
      • OTTHUMP00000195083 antibody
      • OTTHUMP00000195084 antibody
      • Somatomedia C antibody
      • Somatomedin C antibody
      • Somatomedin-C antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 antibody (ab9572)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 antibody (ab9572)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling IGF1 with ab9572 at 1 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.

    • Sandwich ELISA - Anti-IGF1 antibody (ab9572)
      Sandwich ELISA - Anti-IGF1 antibody (ab9572)

      To detect hIGF-I by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-I as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-I.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 antibody (ab9572)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 antibody (ab9572)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling IGF1 with ab9572 at 1 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.

    References (95)

    Publishing research using ab9572? Please let us know so that we can cite the reference in this datasheet.

    ab9572 has been referenced in 95 publications.

    • Xu X  et al. YAP-TEAD up-regulates IRS2 expression to induce and deteriorate oesophageal cancer. J Cell Mol Med 25:2584-2595 (2021). PubMed: 33570213
    • Musicant AM  et al. CRTC1/MAML2 directs a PGC-1a-IGF-1 circuit that confers vulnerability to PPAR? inhibition. Cell Rep 34:108768 (2021). PubMed: 33626346
    • Castillero E  et al. Activin type II receptor ligand signaling inhibition after experimental ischemic heart failure attenuates cardiac remodeling and prevents fibrosis. Am J Physiol Heart Circ Physiol 318:H378-H390 (2020). PubMed: 31886717
    • Lin G  et al. Circular RNA circPLK1 promotes breast cancer cell proliferation, migration and invasion by regulating miR-4500/IGF1 axis. Cancer Cell Int 20:593 (2020). PubMed: 33298061
    • Ma JB  et al. KLF5 inhibits STAT3 activity and tumor metastasis in prostate cancer by suppressing IGF1 transcription cooperatively with HDAC1. Cell Death Dis 11:466 (2020). PubMed: 32546700
    View all Publications for this product

    Customer reviews and Q&As

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    1-10 of 27 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-IGF1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Skeletal muscle (quadriceps))
    Permeabilization
    Yes - Methanol gradient (70% for 10 mins, 95% for 10 mins, 100% for 20 mins, 95% for 10 mins, 70% for 10 mins)
    Specification
    Skeletal muscle (quadriceps)
    Blocking step
    NGS diluted in TBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Valeria Di Leo

    Verified customer

    Submitted Oct 26 2020

    Flow Cytometry abreview for Anti-IGF1 antibody

     Good
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (EL4 T cells tumor line and primary hepatocytes)
    Permeabilization
    No
    Gating Strategy
    Positive cells for FITC
    Specification
    EL4 T cells tumor line and primary hepatocytes
    Preparation
    Cell harvesting/tissue preparation method: Mice were dissected to obtain the liver. The liver was then mashed to obtain hepatocytes.
    Sample buffer: PBS with 10% bovine serum
    Fixation
    none
    Read More

    Safa Mohamad

    Verified customer

    Submitted May 12 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-IGF1 antibody

     Excellent
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (mouse neuron)
    Permeabilization
    Yes - triton X-100
    Specification
    mouse neuron
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 28 2015

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-IGF1 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    None
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 15 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IGF1 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated
    Sample
    Mouse Tissue sections (Heart)
    Specification
    Heart
    Permeabilization
    No
    Fixative
    10% Formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 15 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-IGF1 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cultured Cells (human embryonic stem cells)
    Specification
    human embryonic stem cells
    Fixative
    Methanol
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 13 2013

    Question

    Do you know if this anti-IGF antibody works for flow cytometry applications?

    Read More
    Answer

    To our knowledge, this IGF antibody has not been tested in flow cytometry and our guarantee does not cover this application of the antibody. However it has been tested and shown to work in a similar application, immunocytochemistry, after methanol fixation of the cells. For flow cytometry, your cells will need to be fixed and permeabilized, as IGF is completely intracellular until it is secreted.

    Read More

    Question

    For immunogen of this Ab:
    what was used as expression system for the immunogen?
    Which column type was used (affinity purified, but which column type)?

    Read More
    Answer

    Thank you for contacting us.

    The lab sent me the following information:

    The immunizing antigen was produced in E.coli.
    The purification of this antibody is done using an antigen affinity column.
    This is all the information we have available.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Dear Kate,

    Thank you for your reply. I don’t understand how there can be a guarantee that these antibodies will work in human samples if they have not been tested?

    Please find my replies to your questions below in blue:

    Is there anything you could suggest that I could do to take this further?

    Thank you,

    Read More
    Answer

    Thank you for your message and for providing this further information.

    To confirm, I can confirm this antibody has been tested and therefore guaranteed for human samples. The previous email stated that it has not been tested specifically with serum samples in ELISA. Serum contains many other proteins and other biological factors that may be interfering with the antibody binding.

    I am sorry to hear the suggestions made have not improved the results on this occasion. As previously discussed,the antibodies seem to be workingbut regrettably there seems to be some interference from something within the serum samples. I appreciate the time you have spent on these experiments and as I gesture of goodwill I would be pleased to arrange a credit note. Alternatively, Iwould be pleased to provide free of charge replacements if this is what you prefer and would like to try again. However, please bear in mind it is possible this may not solve the issue if there is a possibility the serum sample type is not suitable for use with these antibodies in ELISA.

    I hope this will provide a satisfactory resolution. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Question

    Dear Kate,



    Thank you for your email. As the antibodies are guaranteed to work with serum samples, what kind of pre-treatment would I need to use in order to get accurate results? What was used during testing for IGF1 ELISA?



    Please find my replies to the questionnaire below in blue:







    Order Details

    Antibody code: ab9572 and ab83137



    Problem

    Choose: weak signal with serum samples but not with standard curve





    Lot number GR21525-6 (ab9572) and GR9925-6 (ab83137)





    Purchase order number HFE2462590-1 (ab83137) and ab9572 was sent for free as per the abpromise

    or preferably Abcam order number





    General Information

    Antibody storage conditions (temperature/reconstitution etc)

    Aliquoted ab9572 in 5ul aliquots and stored at -20C. Reconstituted ab83137 in PBS/0.1% BSA, aliquoted as 5ul aliquots and stored at -20C.



    Description of the problem (high background, no signal, non-specific color development, poor standard curve etc.)



    While the standard curve is good, the pre-treated serum samples are the problem. The serum is from samples that had already been tested using an IGF1 ELISA kit. This serum (from the same tube) was stored at -80C. For the current ELISA, I pre-treated the serum by first treating 30ul of serum with 120ul acid-ethanol (2.5% HCl-87.5% ethanol). This was then incubated at room temperature for 30 minutes with shaking. The samples were then centrifuged for 5 minutes at 10,000rpm and 100ul of supernatant was transferred to a fresh tube containing 200ul Tris Buffer (pH 7.6). The tube was mixed thoroughly and assayed immediately. I also assayed the same serum samples in a 1:15 dilution using PBS to check if the pre-treatment was working. Additionally, I had human serum control (from a bottle from Sigma) as an additional sample. So this was pre-treated and only diluted as well. However, the absorbance and the concentrations calculated from the standard curve for both the pre-treated samples and the diluted samples were similar and these concentrations were about 4-5-fold lower than previously obtained results for the same samples.







    Type of ELISA (Direct ELISA/Indirect ELISA/Sandwich ELISA etc.)

    Sandwich ELISA



    Sample (Species/Cell type/Cell line etc.)

    Serum samples either pre-treated or not.



    Coating well (Buffer/Concentration of the coating material etc.)

    ab9572 was used to coat the plates at 1:1000 dilution in PBS. The coated plates were stored at 4C overnight.



    Blocking conditions (Buffer/time period, Blocking agent etc.)

    5% non-fat milk in PBS solution was used to block the plates for 1 hour.



    Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)

    ab83137 was used as the detection antibody at 1:6000. The plates were incubated at room temperature for 90 minutes. Wash steps were done 6 times in PBS/Tween 20.



    Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)

    Streptavidin-HRP (Sigma) at 1:2000 dilution in PBS. Incubated for 90 minutes, wash 6x in PBS/Tween20.



    Detection method (Substrate/Diluent etc.)

    Citrate buffer with TMB and hydrogen peroxide.



    Positive and negative controls used (please specify)

    The serum samples (both pre-treated and diluted) were used as positive controls. I used two samples, one with a high concentration (˜400 ng/ml) and the other with a lower concentration (˜55 ng/ml). Also, blanks were used.







    Optimization attempts (problem solving)

    How many times have you tried the ELISA?

    Two times with the samples; 5 times with standards and a human serum control (Sigma).





    Have you run a "No Primary" control?

    Yes

    Do you obtain the same results every time?

    The problem is not with the capture and detection antibodies not detecting IGF1 but with very low absorbance/signal for serum samples, either pre-treated or diluted.





    Additional Notes:



    Data:

    We would appreciate if you are able to provide a copy of the data, particularly from the standard curve. This will help us to assess the results.



    I have attached an Excel file with the results from the second test with samples.







    Help us improve our service.

    Rate your experience with us today. <https://www.abcam.com/index.html?pageConfig=technicalSurvey&intCCEID=3782221>



    Best regards,

    Kate



    Kate Hayes

    Scientific Support Supervisor

    Abcam plc

    https://www.abcam.com



    Your original inquiry to Abcam:













    Dear Kate,

    Thank you for your reply. During testing for IGFBP3 and IGF1 antibodies, were the ELISAs tested with serum/plasma samples at all? Do you have any other customers who have used either sets of antibodies to set up a sandwich ELISA? I am having trouble with the IGF1 antibodies with pre-treated serum samples. I was wondering if you have any protocols for serum/plasma pre-treatment to use for this protocol?

    Thank you,









    Abcam Customer Services and Scientific Support Team

    https://www.abcam.com/technical%3chttp:/www.abcam.com/index.html?pageconfig=technical&utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Body

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    Read More
    Answer

    Thank you for your reply

    Please note that theseantibodies have not been tested with with serum samples, and so I am sorry no pretreatments have been tested which we could provide you with information on. In theory the antibodiesshould work with serum samples. Since the antibodies are working well with their standard protein, this means that the antibodies do work in the ELISA.Therefore, I can suggestit may be that there is some interferencewith something in the serum samples.

    1. Have you been able to confirm the expected amount of protein in their samples using any other method?

    2. Is it possible that the samples contain a very small amount of protein?

    3. Is there any azide in the samples? This could inhibit the reactionand would lead to loss ofdetection as you are observing.

    4. Have you tried any other sample types?

    5. Please provide details of the sample preparation.

    I hope this will be helpful. Please do not hesitate to get back to me with the requested information and I hope we can resolve this for you as soon as possible.

    Read More

    1-10 of 27 Abreviews or Q&A

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