- Datasheet
- Specific References (34)
- Protocols
Overview
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Product nameAnti-IGF1 antibody
See all IGF1 primary antibodies -
DescriptionRabbit polyclonal to IGF1
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WB, Neutralising, IHC-FoFr, ICC/IF, Sandwich ELISAmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Highly pure (>98%) recombinant hIGF-1 (human Insulin Like Growth Factor-1).
Properties
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FormLyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPBS, pH 7.4, no preservative, sterile filtered
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Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Light chain typeunknown
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Related Products
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sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab9572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. To detect hIGF-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. | |
| Neutralising | Use at an assay dependent concentration. To yield one-half maximal inhibition of the biological activity of hIGF-1 (5.0 ng/ml), a concentration of 0.67 - 1.0 ug/ml of this antibody is required. | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 28754163 | |
| ICC/IF | 1/200. | |
| Sandwich ELISA | Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IGF1 (Biotin) (ab83137). Allows for the detection of at least 0.2 - 0.4 ng/well of recombinant IGF1. |
Target
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FunctionThe insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.
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Involvement in diseaseDefects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
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Sequence similaritiesBelongs to the insulin family.
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Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 3479 Human
- Entrez Gene: 16000 Mouse
- Omim: 147440 Human
- SwissProt: P05019 Human
- SwissProt: P05017 Mouse
- Unigene: 160562 Human
- Unigene: 268521 Mouse
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FormThere are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA.
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Alternative names
- IBP1 antibody
- IGF I antibody
- IGF IA antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 antibody (ab9572)Ab9572 staining Human normal liver parenchyma. Staining is localised to the cytoplasm.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 antibody (ab9572)This image is courtesy of an anonymous Abreviewab9572 staining IGF1 in mouse heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% formalin and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/1000 in 10% goat serum) for 24 hours at 4°C. An Alexa Fluor® 594-conjugated goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.
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Western blot - Anti-IGF1 antibody (ab9572)This image is courtesy of an anonymous AbreviewAll lanes : Anti-IGF1 antibody (ab9572) at 1/2000 dilution
All lanes : Human vascular smooth muscle whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Donkey Anti-Rabbit IgG - HRP at 1/10000 dilution
Developed using the ECL technique.
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Immunocytochemistry/ Immunofluorescence - Anti-IGF1 antibody (ab9572)This image is courtesy of an anonymous Abreview.ICC/IF image of ab9572 stained human embryonic stem cells. The cells were fixed in methanol and then incubated in 10% BSA for 1 hour to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9572, 1/200) overnight at +4°C. The secondary antibody (red) was an Alexa Fluor® 568 goat anti-rabbit used at a 1/400 dilution.
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Sandwich ELISA - Anti-IGF1 antibody (ab9572)
Protocols
References
This product has been referenced in:
- Ma M et al. Preeclampsia is associated with hypermethylation of IGF-1 promoter mediated by DNMT1. Am J Transl Res 10:16-39 (2018). IHC-P ; Human . Read more (PubMed: 29422991) »
- Hsu PY et al. MicroRNA let-7g inhibits angiotensin II-induced endothelial senescence via the LOX-1-independent mechanism. Int J Mol Med 41:2243-2251 (2018). Read more (PubMed: 29393358) »
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