Anti-IGF1 antibody (ab9572)

Reviews (8)Q&A (19)References (75)
Sandwich ELISA - Anti-IGF1 antibody (ab9572)

    Key features and details

    • Rabbit polyclonal to IGF1
    • Suitable for: Sandwich ELISA
    • Reacts with: Human
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-IGF1 antibody [EPR5098(2)] (ab133542)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-IGF1 antibody
      See all IGF1 primary antibodies

    • Description

      Rabbit polyclonal to IGF1

    • Host species

      Rabbit

    • Tested applications

      Suitable for: Sandwich ELISAmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Highly pure (>98%) recombinant hIGF-1 (human Insulin Like Growth Factor-1).

    • General notes

      Although some customers have been successful in IHC we no longer batch test in this application. For an IHC validated antibody please see ab263903

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab9572 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IGF1 (Biotin) (ab83137). Allows for the detection of at least 0.2 - 0.4 ng/well of recombinant IGF1.

    Target

    • Function

      The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.

    • Involvement in disease

      Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.

    • Sequence similarities

      Belongs to the insulin family.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Form

        There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA.

      • Alternative names

        • IBP1 antibody
        • IGF I antibody
        • IGF IA antibody
        • IGF IB antibody
        • IGF-I antibody
        • Igf1 antibody
        • IGF1_HUMAN antibody
        • IGF1A antibody
        • IGFI antibody
        • IGFIA antibody
        • Insulin like growth factor 1 (somatomedin C) antibody
        • Insulin like growth factor 1 antibody
        • Insulin like growth factor IA antibody
        • Insulin like growth factor IB antibody
        • Insulin-like growth factor I antibody
        • Mechano growth factor antibody
        • MGF antibody
        • OTTHUMP00000195080 antibody
        • OTTHUMP00000195081 antibody
        • OTTHUMP00000195082 antibody
        • OTTHUMP00000195083 antibody
        • OTTHUMP00000195084 antibody
        • Somatomedia C antibody
        • Somatomedin C antibody
        • Somatomedin-C antibody
        see all

      Images

      • Sandwich ELISA - Anti-IGF1 antibody (ab9572)
        Sandwich ELISA - Anti-IGF1 antibody (ab9572)

        To detect hIGF-I by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-I as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-I.

      References (75)

      Publishing research using ab9572? Please let us know so that we can cite the reference in this datasheet.

      ab9572 has been referenced in 75 publications.

      • Shen X  et al. MicroRNA-155 promotes apoptosis of colonic smooth muscle cells and aggravates colonic dysmotility by targeting IGF-1. Exp Ther Med 19:2725-2732 (2020). PubMed: 32256755
      • Ma JB  et al. KLF5 inhibits STAT3 activity and tumor metastasis in prostate cancer by suppressing IGF1 transcription cooperatively with HDAC1. Cell Death Dis 11:466 (2020). PubMed: 32546700
      • Lee C  et al. Magnolol Attenuates Cisplatin-Induced Muscle Wasting by M2c Macrophage Activation. Front Immunol 11:77 (2020). PubMed: 32117241
      • Dey S  et al. Global targetome analysis reveals critical role of miR-29a in pancreatic stellate cell mediated regulation of PDAC tumor microenvironment. BMC Cancer 20:651 (2020). PubMed: 32660466
      • Miyagawa K  et al. Osteoclast-derived IGF1 is required for pagetic lesion formation in vivo. JCI Insight 5:N/A (2020). PubMed: 32078587
      View all Publications for this product

      Customer reviews and Q&As

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      1-10 of 27 Abreviews or Q&A

      Immunohistochemistry (Frozen sections) abreview for Anti-IGF1 antibody

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (Skeletal muscle (quadriceps))
      Permeabilization
      Yes - Methanol gradient (70% for 10 mins, 95% for 10 mins, 100% for 20 mins, 95% for 10 mins, 70% for 10 mins)
      Specification
      Skeletal muscle (quadriceps)
      Blocking step
      NGS diluted in TBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Valeria Di Leo

      Verified customer

      Submitted Oct 26 2020

      Flow Cytometry abreview for Anti-IGF1 antibody

       Good
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Mouse Cell (EL4 T cells tumor line and primary hepatocytes)
      Permeabilization
      No
      Gating Strategy
      Positive cells for FITC
      Specification
      EL4 T cells tumor line and primary hepatocytes
      Preparation
      Cell harvesting/tissue preparation method: Mice were dissected to obtain the liver. The liver was then mashed to obtain hepatocytes.
      Sample buffer: PBS with 10% bovine serum
      Fixation
      none
      Read More

      Safa Mohamad

      Verified customer

      Submitted May 12 2016

      Immunocytochemistry/ Immunofluorescence abreview for Anti-IGF1 antibody

       Excellent
      Abreviews
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (mouse neuron)
      Permeabilization
      Yes - triton X-100
      Specification
      mouse neuron
      Blocking step
      BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 28 2015

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-IGF1 antibody

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Rat Tissue sections (Brain)
      Antigen retrieval step
      None
      Specification
      Brain
      Blocking step
      Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 15 2015

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IGF1 antibody

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
      Antigen retrieval step
      Heat mediated
      Sample
      Mouse Tissue sections (Heart)
      Specification
      Heart
      Permeabilization
      No
      Fixative
      10% Formalin
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 15 2014

      Question

      Do you know if this anti-IGF antibody works for flow cytometry applications?

      Read More
      Answer

      To our knowledge, this IGF antibody has not been tested in flow cytometry and our guarantee does not cover this application of the antibody. However it has been tested and shown to work in a similar application, immunocytochemistry, after methanol fixation of the cells. For flow cytometry, your cells will need to be fixed and permeabilized, as IGF is completely intracellular until it is secreted.

      Read More

      Immunocytochemistry/ Immunofluorescence abreview for Anti-IGF1 antibody

       Good
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cultured Cells (human embryonic stem cells)
      Specification
      human embryonic stem cells
      Fixative
      Methanol
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      Submitted May 13 2013

      Question

      For immunogen of this Ab:
      what was used as expression system for the immunogen?
      Which column type was used (affinity purified, but which column type)?

      Read More
      Answer

      Thank you for contacting us.

      The lab sent me the following information:

      The immunizing antigen was produced in E.coli.
      The purification of this antibody is done using an antigen affinity column.
      This is all the information we have available.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Question

      Dear Kate,

      Thank you for your reply. I don’t understand how there can be a guarantee that these antibodies will work in human samples if they have not been tested?

      Please find my replies to your questions below in blue:

      Is there anything you could suggest that I could do to take this further?

      Thank you,

      Read More
      Answer

      Thank you for your message and for providing this further information.

      To confirm, I can confirm this antibody has been tested and therefore guaranteed for human samples. The previous email stated that it has not been tested specifically with serum samples in ELISA. Serum contains many other proteins and other biological factors that may be interfering with the antibody binding.

      I am sorry to hear the suggestions made have not improved the results on this occasion. As previously discussed,the antibodies seem to be workingbut regrettably there seems to be some interference from something within the serum samples. I appreciate the time you have spent on these experiments and as I gesture of goodwill I would be pleased to arrange a credit note. Alternatively, Iwould be pleased to provide free of charge replacements if this is what you prefer and would like to try again. However, please bear in mind it is possible this may not solve the issue if there is a possibility the serum sample type is not suitable for use with these antibodies in ELISA.

      I hope this will provide a satisfactory resolution. I look forward to hearing from you with details of how you would like to proceed.

      Read More

      Question

      Dear Kate,



      Thank you for your email. As the antibodies are guaranteed to work with serum samples, what kind of pre-treatment would I need to use in order to get accurate results? What was used during testing for IGF1 ELISA?



      Please find my replies to the questionnaire below in blue:







      Order Details

      Antibody code: ab9572 and ab83137



      Problem

      Choose: weak signal with serum samples but not with standard curve





      Lot number GR21525-6 (ab9572) and GR9925-6 (ab83137)





      Purchase order number HFE2462590-1 (ab83137) and ab9572 was sent for free as per the abpromise

      or preferably Abcam order number





      General Information

      Antibody storage conditions (temperature/reconstitution etc)

      Aliquoted ab9572 in 5ul aliquots and stored at -20C. Reconstituted ab83137 in PBS/0.1% BSA, aliquoted as 5ul aliquots and stored at -20C.



      Description of the problem (high background, no signal, non-specific color development, poor standard curve etc.)



      While the standard curve is good, the pre-treated serum samples are the problem. The serum is from samples that had already been tested using an IGF1 ELISA kit. This serum (from the same tube) was stored at -80C. For the current ELISA, I pre-treated the serum by first treating 30ul of serum with 120ul acid-ethanol (2.5% HCl-87.5% ethanol). This was then incubated at room temperature for 30 minutes with shaking. The samples were then centrifuged for 5 minutes at 10,000rpm and 100ul of supernatant was transferred to a fresh tube containing 200ul Tris Buffer (pH 7.6). The tube was mixed thoroughly and assayed immediately. I also assayed the same serum samples in a 1:15 dilution using PBS to check if the pre-treatment was working. Additionally, I had human serum control (from a bottle from Sigma) as an additional sample. So this was pre-treated and only diluted as well. However, the absorbance and the concentrations calculated from the standard curve for both the pre-treated samples and the diluted samples were similar and these concentrations were about 4-5-fold lower than previously obtained results for the same samples.







      Type of ELISA (Direct ELISA/Indirect ELISA/Sandwich ELISA etc.)

      Sandwich ELISA



      Sample (Species/Cell type/Cell line etc.)

      Serum samples either pre-treated or not.



      Coating well (Buffer/Concentration of the coating material etc.)

      ab9572 was used to coat the plates at 1:1000 dilution in PBS. The coated plates were stored at 4C overnight.



      Blocking conditions (Buffer/time period, Blocking agent etc.)

      5% non-fat milk in PBS solution was used to block the plates for 1 hour.



      Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)

      ab83137 was used as the detection antibody at 1:6000. The plates were incubated at room temperature for 90 minutes. Wash steps were done 6 times in PBS/Tween 20.



      Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)

      Streptavidin-HRP (Sigma) at 1:2000 dilution in PBS. Incubated for 90 minutes, wash 6x in PBS/Tween20.



      Detection method (Substrate/Diluent etc.)

      Citrate buffer with TMB and hydrogen peroxide.



      Positive and negative controls used (please specify)

      The serum samples (both pre-treated and diluted) were used as positive controls. I used two samples, one with a high concentration (˜400 ng/ml) and the other with a lower concentration (˜55 ng/ml). Also, blanks were used.







      Optimization attempts (problem solving)

      How many times have you tried the ELISA?

      Two times with the samples; 5 times with standards and a human serum control (Sigma).





      Have you run a "No Primary" control?

      Yes

      Do you obtain the same results every time?

      The problem is not with the capture and detection antibodies not detecting IGF1 but with very low absorbance/signal for serum samples, either pre-treated or diluted.





      Additional Notes:



      Data:

      We would appreciate if you are able to provide a copy of the data, particularly from the standard curve. This will help us to assess the results.



      I have attached an Excel file with the results from the second test with samples.







      Help us improve our service.

      Rate your experience with us today. <https://www.abcam.com/index.html?pageConfig=technicalSurvey&intCCEID=3782221>



      Best regards,

      Kate



      Kate Hayes

      Scientific Support Supervisor

      Abcam plc

      https://www.abcam.com



      Your original inquiry to Abcam:













      Dear Kate,

      Thank you for your reply. During testing for IGFBP3 and IGF1 antibodies, were the ELISAs tested with serum/plasma samples at all? Do you have any other customers who have used either sets of antibodies to set up a sandwich ELISA? I am having trouble with the IGF1 antibodies with pre-treated serum samples. I was wondering if you have any protocols for serum/plasma pre-treatment to use for this protocol?

      Thank you,









      Abcam Customer Services and Scientific Support Team

      https://www.abcam.com/technical%3chttp:/www.abcam.com/index.html?pageconfig=technical&utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Body

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      Read More
      Answer

      Thank you for your reply

      Please note that theseantibodies have not been tested with with serum samples, and so I am sorry no pretreatments have been tested which we could provide you with information on. In theory the antibodiesshould work with serum samples. Since the antibodies are working well with their standard protein, this means that the antibodies do work in the ELISA.Therefore, I can suggestit may be that there is some interferencewith something in the serum samples.

      1. Have you been able to confirm the expected amount of protein in their samples using any other method?

      2. Is it possible that the samples contain a very small amount of protein?

      3. Is there any azide in the samples? This could inhibit the reactionand would lead to loss ofdetection as you are observing.

      4. Have you tried any other sample types?

      5. Please provide details of the sample preparation.

      I hope this will be helpful. Please do not hesitate to get back to me with the requested information and I hope we can resolve this for you as soon as possible.

      Read More

      1-10 of 27 Abreviews or Q&A

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