For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Thank you for your reply. During testing for IGFBP3 and IGF1 antibodies, were the ELISAs tested with serum/plasma samples at all? Do you have any other customers who have used either sets of antibodies to set up a sandwich ELISA? I am having trouble with the IGF1 antibodies with pre-treated serum samples. I was wondering if you have any protocols for serum/plasma pre-treatment to use for this protocol?
Asked on May 21 2012
Thank you for your reply.
I would like to reassure you that ab83137 Anti-IGF1 antibody (Biotin) and ab9572 Anti-IGF1 antibody are tested and covered by our 6 month guarantee for sandwich ELISA together, and human samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.
As the difficulties are continuing, I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.
I would appreciate if you could include details of the sample pretreatment. What was the pretreatment protocol, and the reasons why this was done?
I would appreciate if you could also providethe data from the standard curve and samples which would help us to assess the results.
With regards to the protocol used for testing, both the IGF1 and IGFBP3 antibodies will have been tested using the same procedure. Once I have further information from you, I would like to investigate further this further with our source if they have any other recommendations for you from their experiences with this antibody, particularly with regards to sample type and preparation.
Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.
Choose: Problem with standard curve No signal or weak signal High background
Purchase order number
or preferably Abcam order number
Antibody storage conditions (temperature/reconstitution etc)
Description of the problem (high background, no signal, non-specific color development, poor standard curve etc.)
Type of ELISA (Direct ELISA/Indirect ELISA/Sandwich ELISA etc.)
Sample (Species/Cell type/Cell line etc.)
Coating well (Buffer/Concentration of the coating material etc.)
Blocking conditions (Buffer/time period, Blocking agent etc.)
Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
Detection method (Substrate/Diluent etc.)
Positive and negative controls used (please specify)
Optimization attempts (problem solving)
How many times have you tried the ELISA?
Have you run a "No Primary" control?
Do you obtain the same results every time?
What steps have you altered?
We would appreciate if you are able to provide a copy of the data, particularly from the standard curve. This will help us to assess the results.
Answered on May 21 2012