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Thank you for your reply. I don’t understand how there can be a guarantee that these antibodies will work in human samples if they have not been tested?
Please find my replies to your questions below in blue:
Is there anything you could suggest that I could do to take this further?
Asked on May 28 2012
Thank you for your message and for providing this further information.
To confirm, I can confirm this antibody has been tested and therefore guaranteed for human samples. The previous email stated that it has not been tested specifically with serum samples in ELISA. Serum contains many other proteins and other biological factors that may be interfering with the antibody binding.
I am sorry to hear the suggestions made have not improved the results on this occasion. As previously discussed,the antibodies seem to be workingbut regrettably there seems to be some interference from something within the serum samples. I appreciate the time you have spent on these experiments and as I gesture of goodwill I would be pleased to arrange a credit note. Alternatively, Iwould be pleased to provide free of charge replacements if this is what you prefer and would like to try again. However, please bear in mind it is possible this may not solve the issue if there is a possibility the serum sample type is not suitable for use with these antibodies in ELISA.
I hope this will provide a satisfactory resolution. I look forward to hearing from you with details of how you would like to proceed.
Answered on May 28 2012