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Do you know if this anti-IGF antibody works for flow cytometry applications?
Asked on May 22 2013
To our knowledge, this IGF antibody has not been tested in flow cytometry and our guarantee does not cover this application of the antibody. However it has been tested and shown to work in a similar application, immunocytochemistry, after methanol fixation of the cells. For flow cytometry, your cells will need to be fixed and permeabilized, as IGF is completely intracellular until it is secreted.
Answered on May 22 2013