Recombinant Anti-IGF1 antibody [EPR5098(2)] (ab133542)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5098(2)] to IGF1
- Suitable for: ELISA, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IGF1 antibody [EPR5098(2)]
See all IGF1 primary antibodies -
Description
Rabbit monoclonal [EPR5098(2)] to IGF1 -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IGF1 recombinant protein
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.20 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5098(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
Tested with recombinant protein. |
Notes |
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ELISA
Use at an assay dependent concentration. |
WB
1/1000 - 1/10000. Predicted molecular weight: 22 kDa. Tested with recombinant protein. |
Target
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Function
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
Involvement in disease
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
Sequence similarities
Belongs to the insulin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3479 Human
- Omim: 147440 Human
- SwissProt: P01343 Human
- SwissProt: P05019 Human
- Unigene: 160562 Human
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Form
There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA. -
Alternative names
- IBP1 antibody
- IGF I antibody
- IGF IA antibody
see all
Images
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Anti-IGF1 antibody [EPR5098(2)] (ab133542) at 1/1000 dilution + Recombinant human IGF-I fragment at 0.005 µg
Predicted band size: 22 kDaThe recombinant IGF-I fragment corresponds to amino acids 49-118 of human IGF-1 with the molecular weight of 10kDa.
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Primary antibody, ab33542 was used at concentrations between 0.1 - 1000ng/ml. An Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody at 1:2500 dilution. Antigen concentration was 1000ng/ml.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab133542 has been referenced in 5 publications.
- Fei J et al. Inhibition of SNCG suppresses the proliferation of lung cancer cells induced by high glucose. Mol Med Rep 23:N/A (2021). PubMed: 33313952
- Yin G et al. Exosomal miR-130a-3p promotes the progression of differentiated thyroid cancer by targeting insulin-like growth factor 1. Oncol Lett 21:283 (2021). PubMed: 33732359
- Hou Z et al. PIAS1 alleviates diabetic peripheral neuropathy through SUMOlation of PPAR-γ and miR-124-induced downregulation of EZH2/STAT3. Cell Death Discov 7:372 (2021). PubMed: 34857740
- Chen ZL et al. Elevated Levels of circRUNX1 in Colorectal Cancer Promote Cell Growth and Metastasis via miR-145-5p/IGF1 Signalling. Onco Targets Ther 13:4035-4048 (2020). PubMed: 32494158
- Shi S et al. Human IGF-I propeptide A promotes articular chondrocyte biosynthesis and employs glycosylation-dependent heparin binding. Biochim Biophys Acta 1862:567-575 (2018). PubMed: 29174671