Recombinant Anti-IGF1 antibody [EPR5098(2)] - BSA and Azide free (ab221958)
![Western blot - Anti-IGF1 antibody [EPR5098(2)] - BSA and Azide free (ab221958)](https://www.abcam.com/ps/products/221/ab221958/Images/ab221958-405917-anti-igf1-antibody-epr5098-2-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-IGF1 antibody [EPR5098(2)] - BSA and Azide free (ab221958)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5098(2)] to IGF1 - BSA and Azide free
- Suitable for: ELISA, WB
- Reacts with: Recombinant fragment
Overview
-
Product name
Anti-IGF1 antibody [EPR5098(2)] - BSA and Azide free
See all IGF1 primary antibodies -
Description
Rabbit monoclonal [EPR5098(2)] to IGF1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Recombinant fragment
Predicted to work with: Human
-
Immunogen
Synthetic peptide within Human IGF1 aa 50-150. The exact sequence is proprietary.
Database link: P05019 -
Positive control
- IGF1 recombinant protein
-
General notes
ab221958 is the carrier-free version of ab133542 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab221958 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 4.20 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5098(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab221958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 22 kDa. Tested with recombinant protein. |
Target
-
Function
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
Involvement in disease
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
Sequence similarities
Belongs to the insulin family. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 3479 Human
- Omim: 147440 Human
- SwissProt: P05019 Human
- Unigene: 160562 Human
-
Form
There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA. -
Alternative names
- IBP1 antibody
- IGF I antibody
- IGF IA antibody
see all
Images
-
Western blot - Anti-IGF1 antibody [EPR5098(2)] - BSA and Azide free (ab221958)Anti-IGF1 antibody [EPR5098(2)] (ab133542) at 1/1000 dilution + Recombinant human IGF-I fragment at 0.005 µg
Predicted band size: 22 kDaThis data was developed using ab133542, the same antibody clone in a different buffer formulation.
The recombinant IGF-I fragment corresponds to amino acids 49-118 of human IGF-1 with the molecular weight of 10kDa.
-
![ELISA - Anti-IGF1 antibody [EPR5098(2)] - BSA and Azide free (ab221958)](https://www.abcam.com/ps/products/221/ab221958/Images/ab221958-2-anti-igf1-antibody-epr5098-2-bsa-and-azide-free-elisa.jpg)
ELISA - Anti-IGF1 antibody [EPR5098(2)] - BSA and Azide free (ab221958)This data was developed using ab133542, the same antibody clone in a different buffer formulation.Primary antibody, ab33542 was used at concentrations between 0.1 - 1000ng/ml. An Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody at 1:2500 dilution. Antigen concentration was 1000ng/ml. -
![OI-RD Scanning - Anti-IGF1 antibody [EPR5098(2)] - BSA and Azide free (ab221958)](https://www.abcam.com/ps/products/221/ab221958/Images/ab221958-1-anti-igf1-antibody-epr5098-2-bsa-and-azide-free-equilibrium-disassociation-constant-kd.jpg)
OI-RD Scanning - Anti-IGF1 antibody [EPR5098(2)] - BSA and Azide free (ab221958)This data was developed using ab133542, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD -
![Anti-IGF1 antibody [EPR5098(2)] - BSA and Azide free (ab221958)](https://www.abcam.com/ps/products/221/ab221958/Images/ab221958-4-benefits-of-recombinant-antibodies.png)
Anti-IGF1 antibody [EPR5098(2)] - BSA and Azide free (ab221958)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab221958 has not yet been referenced specifically in any publications.
