Overview

  • Product name

    Anti-IGF1 antibody [EPR5099]
    See all IGF1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5099] to IGF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Recombinant fragment
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide within Human IGF1 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • IGF1 recombinant protein
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20000. Predicted molecular weight: 22 kDa.

Target

  • Function

    The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.
  • Involvement in disease

    Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
  • Sequence similarities

    Belongs to the insulin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Form

    There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA.
  • Alternative names

    • IBP1 antibody
    • IGF I antibody
    • IGF IA antibody
    • IGF IB antibody
    • IGF-I antibody
    • Igf1 antibody
    • IGF1_HUMAN antibody
    • IGF1A antibody
    • IGFI antibody
    • IGFIA antibody
    • Insulin like growth factor 1 (somatomedin C) antibody
    • Insulin like growth factor 1 antibody
    • Insulin like growth factor IA antibody
    • Insulin like growth factor IB antibody
    • Insulin-like growth factor I antibody
    • Mechano growth factor antibody
    • MGF antibody
    • OTTHUMP00000195080 antibody
    • OTTHUMP00000195081 antibody
    • OTTHUMP00000195082 antibody
    • OTTHUMP00000195083 antibody
    • OTTHUMP00000195084 antibody
    • Somatomedia C antibody
    • Somatomedin C antibody
    • Somatomedin-C antibody
    see all

Images

  • Anti-IGF1 antibody [EPR5099] (ab134140) at 1/20000 dilution (purified) + Human IGF-1 recombinant protein at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 10 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-IGF1 antibody [EPR5099] (ab134140) at 1/10000 dilution (unpurified) + IGF1 recombinant protein at 0.005 µg

    Secondary
    Goat Anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 22 kDa

References

ab134140 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab134140.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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