Recombinant Anti-IGF1 antibody [EPR5099] (ab134140)
![Western blot - Anti-IGF1 antibody [EPR5099] (ab134140)](https://www.abcam.com/ps/products/134/ab134140/Images/ab134140-242067-anti-igf1-antibody-epr5099-western-blot.jpg "Western blot - Anti-IGF1 antibody [EPR5099] (ab134140)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5099] to IGF1
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-IGF1 antibody [EPR5099]
See all IGF1 primary antibodies -
Description
Rabbit monoclonal [EPR5099] to IGF1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide within Human IGF1 aa 100-200. The exact sequence is proprietary.
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Positive control
- IGF1 recombinant protein
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5099 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134140 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/20000. Predicted molecular weight: 22 kDa.
|
| Notes |
|---|
|
WB
1/20000. Predicted molecular weight: 22 kDa. |
Target
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Function
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
Involvement in disease
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
Sequence similarities
Belongs to the insulin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3479 Human
- Omim: 147440 Human
- SwissProt: P01343 Human
- SwissProt: P05019 Human
- Unigene: 160562 Human
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Form
There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA. -
Alternative names
- IBP1 antibody
- IGF I antibody
- IGF IA antibody
see all
Images
-
Western blot - Anti-IGF1 antibody [EPR5099] (ab134140)Anti-IGF1 antibody [EPR5099] (ab134140) at 1/20000 dilution (purified) + Human IGF-1 recombinant protein at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 10 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-IGF1 antibody [EPR5099] (ab134140)](https://www.abcam.com/ps/products/134/ab134140/Images/ab134140-148390-anti-igf1-antibody-epr5099-western-blot.jpg)
Western blot - Anti-IGF1 antibody [EPR5099] (ab134140)Anti-IGF1 antibody [EPR5099] (ab134140) at 1/10000 dilution (unpurified) + IGF1 recombinant protein at 0.005 µg
Secondary
Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 22 kDa -
![Anti-IGF1 antibody [EPR5099] (ab134140)](https://www.abcam.com/ps/products/134/ab134140/Images/ab134140-3-benefits-of-recombinant-antibodies.png)
Anti-IGF1 antibody [EPR5099] (ab134140)
Datasheets and documents
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Datasheet download
References (1)
ab134140 has been referenced in 1 publication.
- O'Connor DM et al. Lot-to-Lot Variation in Adeno-Associated Virus Serotype 9 (AAV9) Preparations. Hum Gene Ther Methods 30:214-225 (2019). PubMed: 31752530
