Western blot abreview for Anti-IGF1 Receptor (phospho Y1158 + Y1162 + Y1163) antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (placenta)
Loading amount
75 µg
Specification
placenta
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 2.5% milk in TBS-T

Secondary antibody

Name
Non-Abcam antibody was used: Rabbit IgG, HRP-Linked Whole Ab (from donkey)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
40 minute(s) and 0 second(s)
Bands
Specific: 130 kDa Non-specific: 80 kDa

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Submitted Sep 07 2009

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