Synthetic phospho-peptide derived from human IGF1 receptor around the phosphorylation site of tyrosine 1161
Our Abpromise guarantee covers the use of ab39398 in the following tested applications.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-FoFr||1/100. PubMed: 18216249|
|ICC||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/1000. Predicted molecular weight: 155 kDa.|
|IP||Use at an assay dependent concentration.|
|IHC-FrFl||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
Western blot analysis using IGF1 Receptor antibody (ab39675, Lane 1, 2 and 3) and IGF1 Receptor (phospho-Tyr1161) antibody (ab39398, Lane 4 and 5).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue sections labeling IGF1 Receptor (phospho Y1161) with ab39398 at 1/100 dilution. The tissue was fixed with formaldehyde; heat mediated antigen retrieval was performed using a citrate buffer pH 6.0. The tissue was blocked with 5% serum for 1 hour at 23°C followed by incubation with ab39398 at 1/100 for 1 hour at 23°C. An undiluted polyclonal goat anti-rabbit HRP conjugated secondary antibody was used.