The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IGF2 (Biotin) (ab83142). Using ab83142 as the detection antibody and ab109789 as the capture antibody, the detection limit for recombinant Human IGF2 is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.
Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IGF2.
Use a concentration of 3.1 - 4.6 µg/ml. to yield one-half maximal inhibition [ND50] of the biological activity of IGF2 (1.0 ng/ml)
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 20 kDa. The detection limit for recombinant Human IGF2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions
Use a concentration of 1 - 5 µg/ml.
Use at an assay dependent concentration.
The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development. Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
Involvement in disease
Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations.
Belongs to the insulin family.
O-glycosylated with a core 1 or possibly core 8 glycan.
Hartnell A et al. Identification of selective basophil chemoattractants in human nasal polyps as insulin-like growth factor-1 and insulin-like growth factor-2. J Immunol173:6448-57 (2004).
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