Key features and details
- Rabbit polyclonal to IGF2
- Suitable for: Sandwich ELISA, WB, Neutralising, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IGF2 antibody
See all IGF2 primary antibodies
DescriptionRabbit polyclonal to IGF2
Tested applicationsSuitable for: Sandwich ELISA, WB, Neutralising, ELISAmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human IGF2. 67 amino acid residues
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
sELISA pair antibody
Our Abpromise guarantee covers the use of ab18954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Rat monoclonal [RM0100-3J42] to IGF2 (ab86485).
For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab86485 as Capture.
|WB||Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 20 kDa.|
|Neutralising||Use a concentration of 0.5 - 4 µg/ml.|
|ELISA||Use a concentration of 2 - 3 µg/ml.|
FunctionThe insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
Involvement in diseaseEpigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations.
Sequence similaritiesBelongs to the insulin family.
modificationsO-glycosylated with a core 1 or possibly core 8 glycan.
- Information by UniProt
- C11orf43 antibody
- IGF 2 antibody
- IGF II antibody
Western blot analysis of IGF-II using recombinant human IGF-II.
Lane 2;250 ng
Lane 3;500 ng
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab18954 has not yet been referenced specifically in any publications.