Product nameAnti-IGF2 antibody
See all IGF2 primary antibodies
DescriptionRabbit polyclonal to IGF2
Tested applicationsSuitable for: Sandwich ELISA, WB, Neutralising, ELISAmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human IGF2. 67 amino acid residues
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Thimerosal (merthiolate).
Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.2.
Concentration information loading...
PurityImmunogen affinity purified
sELISA pair antibody
Our Abpromise guarantee covers the use of ab18954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Rat monoclonal [RM0100-3J42] to IGF2 (ab86485).
For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab86485 as Capture.
|WB||Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 20 kDa.|
|Neutralising||Use a concentration of 0.5 - 4 µg/ml.|
|ELISA||Use a concentration of 2 - 3 µg/ml.|
FunctionThe insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
Involvement in diseaseEpigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations.
Sequence similaritiesBelongs to the insulin family.
modificationsO-glycosylated with a core 1 or possibly core 8 glycan.
- Information by UniProt
- C11orf43 antibody
- IGF 2 antibody
- IGF II antibody
Western blot analysis of IGF-II using recombinant human IGF-II.
Lane 2;250 ng
Lane 3;500 ng
ab18954 has not yet been referenced specifically in any publications.