Anti-IGF2 antibody (ab18954)
Key features and details
- Rabbit polyclonal to IGF2
- Suitable for: Sandwich ELISA, WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-IGF2 antibody
See all IGF2 primary antibodies -
Description
Rabbit polyclonal to IGF2 -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant full length protein corresponding to Human IGF2. 67 amino acid residues
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General notes
This product is manufactured by BioVision, an Abcam company and was previously called 5122 IGF-II Antibody. 5122-100 is the same size as the 100 µg size of ab18954.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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sELISA pair antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Rat monoclonal [RM0100-3J42] to IGF2 (ab86485).
For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab86485 as Capture. |
|
WB |
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 20 kDa.
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Notes |
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Sandwich ELISA
Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Rat monoclonal [RM0100-3J42] to IGF2 (ab86485). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab86485 as Capture. |
WB
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 20 kDa. |
Target
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Function
The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3. -
Involvement in disease
Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. -
Sequence similarities
Belongs to the insulin family. -
Post-translational
modificationsO-glycosylated with a core 1 or possibly core 8 glycan. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C11orf43 antibody
- IGF 2 antibody
- IGF II antibody
see all
Images
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Western blot analysis of IGF-II using recombinant human IGF-II.
Lane 1;50ng
Lane 2;250 ng
Lane 3;500 ng
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab18954 has been referenced in 1 publication.
- Liu G et al. Lnc-MCEI mediated the chemosensitivity of esophageal squamous cell carcinoma via miR-6759-5p to competitively regulate IGF2. Int J Biol Sci 16:2938-2950 (2020). PubMed: 33061807