Overview

  • Product name
  • Description
    Rabbit polyclonal to IGF2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, Neutralising, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Cow, Human
  • Immunogen

    Highly pure (>98%) recombinant hIGF-2 (human Insulin Like Growth Factor-2).

  • Positive control
    • ab9575 (Active IGF2 full length protein) can be used as a positive control.
  • General notes


    Insulin Like Growth Factor-2 (IGF-2) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types

Properties

Applications

Our Abpromise guarantee covers the use of ab9574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. To detect hIGF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect hIGF-2 by ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIGF-2.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition of the biological activity of hIGF-2 (10.0 ng/ml), a concentration of 3.1 - 4.6 µg/ml of this antibody is required.
ICC/IF Use a concentration of 1 - 5 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
    Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
  • Involvement in disease
    Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations.
  • Sequence similarities
    Belongs to the insulin family.
  • Post-translational
    modifications
    O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C11orf43 antibody
    • IGF 2 antibody
    • IGF II antibody
    • IGF-II antibody
    • IGF2 antibody
    • IGF2_HUMAN antibody
    • IGFII antibody
    • INSIGF antibody
    • insulin like growth factor 2 (somatomedin A) antibody
    • Insulin like Growth Factor 2 antibody
    • Insulin like growth factor II antibody
    • Insulin like growth factor II precursor antibody
    • Insulin like growth factor type 2 antibody
    • pp9974 antibody
    • Preptin antibody
    • putative insulin like growth factor II associated protein antibody
    • Somatomedin A antibody
    • Somatomedin-A antibody
    see all

Images

  • Immunohistochemical analysis of parafin embedded rat kidney tissue labelling IGF2 with ab9574 at 1ug/ml. 

    See Abreview

  • ICC/IF image of ab9574 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9574, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Rabbit Anti-Human IGF-II against Human IGF-II. Antibody concentration: 0.10 ug/ml Lower limit of detection: 3.91 ng/lane Molecular Weight: 7.5 kDa
    Lane 1: Marker
    Lane 2: 250 ng
    Lane 3: 125 ng
    Lane 4: 62.5 ng
    Lane 5: 31.25 ng
    Lane 6: 15.625 ng
    Lane 7: 7.8 ng
    Lane 8: 3.9 ng

     

  •  ab9574 staining human tongue carcinoma tissue sections by IHC-P. For antigen retrieval sections were placed in a bath of 0.01M citric acid, pH6, and were heated on full power in a microwave for 5 minutes and then for a further 4 minutes. The slides were then cooled and placed in PBS, fixed with paraformaldehyde and blocked with BSA. The slides were incubated with the antibody for 18 hours. An HRP conjugated pig polyclonal antibody was used as the secondary.

    See Abreview

  • IHC-P of normal human placenta stained with ab9574. The recommended concentration ranges from 0.5 µg/ml-5.0 µg/ml.  An HRP-labeled polymer detection system was used with DAB chromogen.  Recommended antigen retrievals are heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer or enzyme induced antigen retrieval with Proteinase K.  Optimal concentrations and conditions may vary.

References

This product has been referenced in:
  • Van Arsdale AR  et al. Insulin-like growth factor 2: a poor prognostic biomarker linked to racial disparity in women with uterine carcinosarcoma. Cancer Med 7:616-625 (2018). IHC-P ; Human . Read more (PubMed: 29455465) »
  • Ireland L  et al. Blockade of insulin-like growth factors increases efficacy of paclitaxel in metastatic breast cancer. Oncogene 37:2022-2036 (2018). IHC-P . Read more (PubMed: 29367764) »
See all 55 Publications for this product

Customer reviews and Q&As

1-10 of 40 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
Permeabilization
No
Specification
lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 21°C
Fixative
10% normal buffered formalin

Abcam user community

Verified customer

Submitted Nov 02 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
10% formalin

Abcam user community

Verified customer

Submitted Sep 21 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
10% formalin

Abcam user community

Verified customer

Submitted Sep 21 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
tumor
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
10% normal buffered formalin

Abcam user community

Verified customer

Submitted Sep 21 2018

Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
5 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 05 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293)
Permeabilization
Yes - 0.1% triton X100
Specification
HEK293
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 18 2017

Application
Immunocytochemistry
Sample
Human Cultured Cells (PULP)
Permeabilization
Yes - tritonx100
Specification
PULP
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde

Kyungjoo Seong

Verified customer

Submitted Nov 30 2016

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 02 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Mouse Cell (primary hepatocytes)
Specification
primary hepatocytes
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (15%)
Sample
Mouse Serum (primary hepatoctyes)
Specification
primary hepatoctyes
Blocking step
I-Block(Applied biosystems) as blocking agent for 30 minute(s) · Concentration: 2µg/mL · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jul 02 2013

1-10 of 40 Abreviews or Q&A

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