Recombinant Anti-IGF2 antibody [EPR12221] (ab177467)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12221] to IGF2
- Suitable for: ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IGF2 antibody [EPR12221]
See all IGF2 primary antibodies -
Description
Rabbit monoclonal [EPR12221] to IGF2 -
Host species
Rabbit -
Specificity
ab177467 has been found not to cross react with mouse or rat IGF-2 in western blot testing. -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human IGF2 aa 50-150 (internal sequence). The exact sequence is proprietary.
Database link: P01344 -
Positive control
- Recombinant human IGF2 protein (ab9575) can be used as a positive control in WB. Human serum lysate and HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12221 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab177467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/50 - 1/100.
|
|
WB |
1/1000 - 1/5000. Predicted molecular weight: 20 kDa.
|
Notes |
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ICC/IF
1/50 - 1/100. |
WB
1/1000 - 1/5000. Predicted molecular weight: 20 kDa. |
Target
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Function
The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3. -
Involvement in disease
Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. -
Sequence similarities
Belongs to the insulin family. -
Post-translational
modificationsO-glycosylated with a core 1 or possibly core 8 glycan. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3481 Human
- Omim: 147470 Human
- SwissProt: P01344 Human
- Unigene: 272259 Human
- Unigene: 718427 Human
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Alternative names
- C11orf43 antibody
- IGF 2 antibody
- IGF II antibody
see all
Images
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Immunofluorescence analysis of HepG2 cells labeling IGF2 (green) with ab177467 at 1/50 dilution. DAPI nuclear staining (blue).
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Anti-IGF2 antibody [EPR12221] (ab177467) at 1/1000 dilution + Human serum lysate at 10 µg
Secondary
Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 20 kDa
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab177467 has not yet been referenced specifically in any publications.