Product nameAnti-IGF2 antibody [mAbcam63294]
See all IGF2 primary antibodies
DescriptionMouse monoclonal [mAbcam63294] to IGF2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human IGF2 aa 150 to the C-terminus.
(Peptide available as
- This antibody gave a positive signal with a full-length, recombinant IGF2 protein.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
Our Abpromise guarantee covers the use of ab63294 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).|
FunctionThe insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
Involvement in diseaseEpigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations.
Sequence similaritiesBelongs to the insulin family.
modificationsO-glycosylated with a core 1 or possibly core 8 glycan.
- Information by UniProt
- C11orf43 antibody
- IGF 2 antibody
- IGF II antibody
Anti-IGF2 antibody [mAbcam63294] (ab63294) at 1 µg/ml + IGF2 Human Recombinant Protein at 0.1 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 46 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Human full length recombinant protein with GST tag (MW-46 kDa with tag).
This product has been referenced in:
- Leslie K et al. Increased apoptosis, altered oxygen signaling, and antioxidant defenses in first-trimester pregnancies with high-resistance uterine artery blood flow. Am J Pathol 185:2731-41 (2015). WB ; Human . Read more (PubMed: 26362067) »