Anti-IGF2 antibody [RM0100-3J42] (ab86485)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-IGF2 antibody [RM0100-3J42]
See all IGF2 primary antibodies -
Description
Rat monoclonal [RM0100-3J42] to IGF2 -
Host species
Rat -
Tested applications
Suitable for: Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length Mouse protein
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purification notes
The IgG fraction of culture supernatant was purified by protein A/G affinity chromatography and 0.2µm filtered. -
Clonality
Monoclonal -
Clone number
RM0100-3J42 -
Isotype
IgG2 -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
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sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab86485 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IGF2 (ab18954). For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab18954 as Detection. |
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| WB | 1/500 - 1/1000. Predicted molecular weight: 20 kDa. |
Target
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Function
The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3. -
Involvement in disease
Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. -
Sequence similarities
Belongs to the insulin family. -
Post-translational
modificationsO-glycosylated with a core 1 or possibly core 8 glycan. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3481 Human
- Entrez Gene: 16002 Mouse
- Omim: 147470 Human
- SwissProt: P01344 Human
- SwissProt: P09535 Mouse
- Unigene: 272259 Human
- Unigene: 718427 Human
- Unigene: 3862 Mouse
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Alternative names
- C11orf43 antibody
- IGF 2 antibody
- IGF II antibody
see all
Images
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![Sandwich ELISA - Anti-IGF2 antibody [RM0100-3J42] (ab86485)](https://www.abcam.com/ps/products/86/ab86485/Images/IGF2-Primary-antibodies-ab86485-3.jpg)
Sandwich ELISA - Anti-IGF2 antibody [RM0100-3J42] (ab86485)Standard Curve for IGF2 (Analyte: ab9575) dilution range 1pg/ml to 1ug/ml using Capture Antibody Rat monoclonal [RM0100-3J42] to IGF2 (ab86485) at 1ug/ml and Detector Antibody Rabbit polyclonal to IGF2 (ab18954) at 0.5ug/ml.
Protocols
Datasheets and documents
References
ab86485 has not yet been referenced specifically in any publications.
