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  1. Link

    igf2-antibody-rm0100-3j42-ab86485.pdf

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Signal Transduction Growth Factors/Hormones Insulin / Insulin-like
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Anti-IGF2 antibody [RM0100-3J42] (ab86485)

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  • Sandwich ELISA - Anti-IGF2 antibody [RM0100-3J42] (ab86485)

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Primary

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Protein

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-IGF2 antibody [RM0100-3J42]
    See all IGF2 primary antibodies
  • Description

    Rat monoclonal [RM0100-3J42] to IGF2
  • Host species

    Rat
  • Tested applications

    Suitable for: Sandwich ELISA, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length Mouse protein

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purification notes

    The IgG fraction of culture supernatant was purified by protein A/G affinity chromatography and 0.2µm filtered.
  • Clonality

    Monoclonal
  • Clone number

    RM0100-3J42
  • Isotype

    IgG2
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Insulin / Insulin-like
    • Cancer
    • Growth factors
    • Insulin and insulin-like
    • Developmental Biology
    • Post embryonic development
    • Growth hormones

Associated products

  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Recombinant Protein

    • Recombinant human IGF2 protein (ab9575)
  • sELISA pair antibody

    • Anti-IGF2 antibody (ab18954)

Applications

Our Abpromise guarantee covers the use of ab86485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IGF2 (ab18954).

For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab18954 as Detection.

WB 1/500 - 1/1000. Predicted molecular weight: 20 kDa.

Target

  • Function

    The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
    Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
  • Involvement in disease

    Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations.
  • Sequence similarities

    Belongs to the insulin family.
  • Post-translational
    modifications

    O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P01344 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3481 Human
    • Entrez Gene: 16002 Mouse
    • Omim: 147470 Human
    • SwissProt: P01344 Human
    • SwissProt: P09535 Mouse
    • Unigene: 272259 Human
    • Unigene: 718427 Human
    • Unigene: 3862 Mouse
    • Alternative names

      • C11orf43 antibody
      • IGF 2 antibody
      • IGF II antibody
      • IGF-II antibody
      • IGF2 antibody
      • IGF2_HUMAN antibody
      • IGFII antibody
      • INSIGF antibody
      • insulin like growth factor 2 (somatomedin A) antibody
      • Insulin like Growth Factor 2 antibody
      • Insulin like growth factor II antibody
      • Insulin like growth factor II precursor antibody
      • Insulin like growth factor type 2 antibody
      • pp9974 antibody
      • Preptin antibody
      • putative insulin like growth factor II associated protein antibody
      • Somatomedin A antibody
      • Somatomedin-A antibody
      see all

    Images

    • Sandwich ELISA - Anti-IGF2 antibody [RM0100-3J42] (ab86485)
      Sandwich ELISA - Anti-IGF2 antibody [RM0100-3J42] (ab86485)
      Standard Curve for IGF2 (Analyte: ab9575) dilution range 1pg/ml to 1ug/ml using Capture Antibody Rat monoclonal [RM0100-3J42] to IGF2 (ab86485) at 1ug/ml and Detector Antibody Rabbit polyclonal to IGF2 (ab18954) at 0.5ug/ml.

    Protocols

    • Western blot protocols

    Datasheets and documents

    • Datasheet
  • References

    ab86485 has not yet been referenced specifically in any publications.

    Publishing research using ab86485? Please let us know so that we can cite the reference in this datasheet.

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