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  1. Link

    igf2bp1imp1-antibody-ab100999.pdf

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Epigenetics and Nuclear Signaling Nuclear Signaling Pathways Nuclear Receptors Nuclear Pore Complex
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Anti-IGF2BP1/IMP1 antibody (ab100999)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (1)

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Western blot - Anti-IGF2BP1/IMP1 antibody (ab100999)
  • Immunocytochemistry/ Immunofluorescence - Anti-IGF2BP1/IMP1 antibody (ab100999)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1/IMP1 antibody (ab100999)

Key features and details

  • Rabbit polyclonal to IGF2BP1/IMP1
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-IGF2BP1/IMP1 antibody
    See all IGF2BP1/IMP1 primary antibodies
  • Description

    Rabbit polyclonal to IGF2BP1/IMP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human IGF2BP1/IMP1 aa 1-100 conjugated to keyhole limpet haemocyanin.
    Database link: Q9NZI8
    (Peptide available as ab113307)

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Human fetal heart; human fetal lung; Human fetal testis; Human fetal liver; Human fetal kidney. This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.
  • General notes

     This product was previously labelled as IGF2BP1

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Nuclear Pore Complex

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab100999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 64 kDa).

Target

  • Function

    RNA-binding factor that affects mRNA nuclear export, localization, stability and translation. Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Regulates mRNA stability during the integrated cellular stress response (ISR) in stress granules (SGs). Stabilizes the BTRC/FBW1A mRNA from degradation by disrupting miRNA-dependent interaction with AGO2. Identified in a HCV IRES-mediated translation complex, that enhances translation at the Hepatitis C virus (HCV) RNA-replicon via the internal ribosome entry site (IRES), but does not affect 5'cap-dependent translation. Acts as a HIV-1 retrovirus restriction factor that reduces HIV-1 assembly by inhibiting viral RNA packaging, assembly and processing of HIV-1 GAG protein on cellular membranes. Binds to mRNAs in stress granules (SGs). Binds to the stem-loop IV of the 5'-UTR and to the variable region and the poly(U-C) motif of the 3'-UTR of the HCV RNA-replicon. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulates its subcellular localization and translation. Binds both to the coding region mRNA stability determinant (CRD) and to AU-rich sequences in the 3'-UTR of the MYC and CD44 mRNAs and stabilizes these mRNAs. Binds to the fourth and fifth exons of the oncofetal H19 and neuron-specific TAU mRNAs and regulates their localizations. Binds to the adenine-rich autoregulatory sequence (ARS) 5'-UTR of the PABPC1 mRNA and is involved in its translational repression. The RNA-binding activity to ARS is stimulated by PABPC1. Binds to the coding sequence region of BTRC/FBW1A mRNA and mediates stabilization of BTRC/FBW1A and MYC mRNAs in response to beta-catenin signaling. Binding to RNA employs a cooperative, sequential mechanism of homo- or heterodimerisation. Also involved in growth or survival of lung-cancer cells. Protects the MYC and MDR-1 mRNAs from cleavage by a endoribonuclease, thus prolonging their stabilities (By similarity). Binds to the 3'-UTR axonal localization signal (ALS) of TAU mRNA (By similarity). Binds to a conserved 54-nucleotide element in the 3'-UTR of the beta actin mRNA known as the 'zipcode' (By similarity). Promotes translocation of the beta-actin mRNA to dendrites (By similarity). May act as a regulator of mRNA transport to activated synapses in response to synaptic activity.
  • Tissue specificity

    Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary, gonocytes of testis, oocytes, spermatogonia and semen (at protein level). Expressed in testicular and lung cancer (at protein level). Expressed in kidney, prostate, trachea, testis and lung cancer.
  • Sequence similarities

    Belongs to the RRM IMP/VICKZ family.
    Contains 4 KH domains.
    Contains 2 RRM (RNA recognition motif) domains.
  • Domain

    The third and fourth KH domains encompass the protein dimerization motif and are necessary and sufficient for RNA binding. The four KH domains are important for granule formation and SGs targeting. Contains two nuclear export signals, situated within the second and fourth KH domains. The four KH domains are important to suppress HIV-1 infectivity.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation may influence mRNA translation.
  • Cellular localization

    Nucleus. Cytoplasm. Cell projection > lamellipodium. Cell projection > dendrite. Cell projection > dendritic spine. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Targeted to stress granules (SGs), but not processing bodies (PBs), during cellular stress. Colocalizes with G3BP1 and TIAL1 in SGs. Colocalizes with HIV-1 GAG at the cell edges. Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Colocalized with H19 RNA at lamellipodia. Colocalized with CD44 mRNA in RNP granules. Nuclear export is mediated by XPO1/CRM1. In motile cells, is transported towards the leading edge into the cortical region of the lamellipodia where it is connected to microfilaments (By similarity). Present in the form of granules and into F-actin-rich protrusion of dendrites, spines and subsynaptic sites (By similarity). Colocalizes with beta-actin mRNA in dendrites and spines (By similarity). Exhibited rapid, bidirectional movements in dendrites and spines (By similarity). Neuronal depolarization by KCl induces its rapid efflux from the cell body into dendrites.
  • Target information above from: UniProt accession Q9NZI8 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 516853 Cow
    • Entrez Gene: 491064 Dog
    • Entrez Gene: 10642 Human
    • Entrez Gene: 140486 Mouse
    • Entrez Gene: 303477 Rat
    • Omim: 608288 Human
    • SwissProt: Q9NZI8 Human
    • SwissProt: O88477 Mouse
    • SwissProt: Q8CGX0 Rat
    • Unigene: 144936 Human
    • Unigene: 294769 Mouse
    • Unigene: 485325 Mouse
    • Unigene: 77572 Rat
    see all
  • Alternative names

    • Coding region determinant-binding protein antibody
    • CRD BP antibody
    • CRD-BP antibody
    • CRDBP antibody
    • IF2B1 antibody
    • IF2B1_HUMAN antibody
    • IGF II mRNA binding protein 1 antibody
    • IGF-II mRNA-binding protein 1 antibody
    • IGF2 mRNA binding protein 1 antibody
    • IGF2 mRNA-binding protein 1 antibody
    • IGF2BP1 antibody
    • IMP 1 antibody
    • IMP-1 antibody
    • IMP1 antibody
    • Insulin like growth factor 2 mRNA binding protein 1 antibody
    • Insulin-like growth factor 2 mRNA-binding protein 1 antibody
    • VICKZ family member 1 antibody
    • VICKZ1 antibody
    • ZBP 1 antibody
    • ZBP-1 antibody
    • ZBP1 antibody
    • Zip code binding protein 1 antibody
    • Zip code-binding protein 1 antibody
    • Zipcode binding protein 1 antibody
    • Zipcode-binding protein 1 antibody
    see all

Images

  • Western blot - Anti-IGF2BP1/IMP1 antibody (ab100999)
    Western blot - Anti-IGF2BP1/IMP1 antibody (ab100999)
    All lanes : Anti-IGF2BP1/IMP1 antibody (ab100999) at 1 µg/ml

    Lane 1 : Heart (Human) Tissue Lysate - fetal normal tissue
    Lane 2 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)
    Lane 3 : Testis (Human) Tissue Lysate - fetal normal tissue
    Lane 4 : Liver (Human) Tissue Lysate - fetal normal tissue
    Lane 5 : Kidney (Human) Tissue Lysate - fetal normal tissue (ab30204)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 64 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes


    The predicted molecular weight of IGF2BP1/IMP1 is 63 kDa (SwissProt), however we expect to observe a banding pattern at 70 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

  • Immunocytochemistry/ Immunofluorescence - Anti-IGF2BP1/IMP1 antibody (ab100999)
    Immunocytochemistry/ Immunofluorescence - Anti-IGF2BP1/IMP1 antibody (ab100999)
    ICC/IF image of ab100999 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab100999, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) mcf7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HepG2 and MCF7 cells at 5µg/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1/IMP1 antibody (ab100999)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1/IMP1 antibody (ab100999)

    IHC image of IGF2BP1/IMP1 staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab100999, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab100999? Please let us know so that we can cite the reference in this datasheet.

    ab100999 has been referenced in 1 publication.

    • Xu Y  et al. Modulation of IGF2BP1 by long non-coding RNA HCG11 suppresses apoptosis of hepatocellular carcinoma cells via MAPK signaling transduction. Int J Oncol 51:791-800 (2017). PubMed: 28677801

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-IGF2BP1/IMP1 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Bos taurus Cell lysate - other (HEK 293T and BoMAC (SV40-transformed bovine macrop)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    15 µg
    Specification
    HEK 293T and BoMAC (SV40-transformed bovine macrop
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 19 2017

    Question



    Would you please send me the SDS or letter indicating no SDS is required for Anti-IGF2BP1 antibody (ab100999).

    Read More

    Abcam community

    Verified customer

    Asked on Jul 18 2012

    Answer

    Thank you for your inquiry.
    This product does require an SDS.
    Our Products team is in the process of updating all datasheets.
    You should be able to see the link online in a few hours (upper right corner).
    But I've also pasted it below:
    https://www.abcam.com/ps/Products/100/ab100999/Msds/ab100999_WPNA_EN.pdf

    I hope this information helps. Please contact us with any other questions.

    Read More

    Abcam Scientific Support

    Answered on Jul 18 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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