Overview

  • Product name
    Anti-IGF2BP2 / IMP-2 antibody [1E3.01E5]
    See all IGF2BP2 / IMP-2 primary antibodies
  • Description
    Mouse monoclonal [1E3.01E5] to IGF2BP2 / IMP-2
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human IGF2BP2/ IMP-2.

  • Positive control
    • HeLa and Jurkat whole cell lysates.
  • General notes

    Previously labelled as IGF2BP2. 

Properties

Applications

Our Abpromise guarantee covers the use of ab128175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 66 kDa.
IP Use at 20 µg/mg of lysate.

Target

  • Function
    Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs.
  • Tissue specificity
    Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis.
  • Sequence similarities
    Belongs to the RRM IMP/VICKZ family.
    Contains 4 KH domains.
    Contains 2 RRM (RNA recognition motif) domains.
  • Domain
    The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA.
  • Cellular localization
    Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa.
  • Information by UniProt
  • Database links
  • Alternative names
    • Hepatocellular carcinoma autoantigen p62 antibody
    • IF2B2_HUMAN antibody
    • IGF 2 mRNA binding protein 2 antibody
    • IGF II mRNA binding protein 2 antibody
    • IGF-II mRNA-binding protein 2 antibody
    • IGF2 mRNA-binding protein 2 antibody
    • Igf2bp2 antibody
    • IMP 2 antibody
    • IMP-2 antibody
    • IMP2 antibody
    • Insulin like growth factor 2 mRNA binding protein 2 antibody
    • Insulin-like growth factor 2 mRNA-binding protein 2 antibody
    • OTTHUMP00000082606 antibody
    • OTTHUMP00000082607 antibody
    • OTTHUMP00000082608 antibody
    • p62 antibody
    • VICKZ 2 antibody
    • VICKZ family member 2 antibody
    • VICKZ2 antibody
    see all

Images

  • All lanes : Anti-IGF2BP2 / IMP-2 antibody [1E3.01E5] (ab128175) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 66 kDa


    Exposure time: 10 seconds
  • Detection of IGF2BP2 / IMP-2 by Western Blot of Immunprecipitate.

    ab128175 at 0.04 µg/ml staining IGF2BP2 / IMP-2 in HeLa whole cell lysate immunoprecipitated using ab128175 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

    Detection: Chemiluminescence with exposure time of 10 seconds.

References

This product has been referenced in:
  • Ye S  et al. IGF2BP2 promotes colorectal cancer cell proliferation and survival through interfering with RAF-1 degradation by miR-195. FEBS Lett 590:1641-50 (2016). Read more (PubMed: 27153315) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you very much for your interest in ab128175.

To our knowledge, ab128175 has not been tested in RIP. Therefore, I can offer a discount off a future purchase if you buy ab128175 now, test it in RIP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab128175 in RIP. I will then send a discount code. This code must be issued before purchasing ab128175 so please wait for my reply before ordering.

2. Purchase ab128175 either by phone, fax, or online (www.abcam.com).

3. Test it in RIP.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab128175 turns out to be unsuitable for RIP, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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