Product nameAnti-IGF2BP2 / IMP-2 antibody
See all IGF2BP2 / IMP-2 primary antibodies
DescriptionRabbit polyclonal to IGF2BP2 / IMP-2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla
Synthetic peptide corresponding to Human IGF2BP2/ IMP-2 aa 549-599.
Database link: NP_006539.3
- Whole cell lysate from 293T, Jurkat and HeLa cells.
Previously labelled as IGF2BP2.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab117809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 66 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionBinds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs.
Tissue specificityExpressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis.
Sequence similaritiesBelongs to the RRM IMP/VICKZ family.
Contains 4 KH domains.
Contains 2 RRM (RNA recognition motif) domains.
DomainThe third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA.
Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa.
- Information by UniProt
- Hepatocellular carcinoma autoantigen p62 antibody
- IF2B2_HUMAN antibody
- IGF 2 mRNA binding protein 2 antibody
All lanes : Anti-IGF2BP2 / IMP-2 antibody (ab117809) at 0.1 µg/ml
Lane 1 : Whole cell lysate from 293T cells at 50 µg
Lane 2 : Whole cell lysate from 293T cells at 15 µg
Lane 3 : Whole cell lysate from Jurkat cells at 50 µg
Lane 4 : Whole cell lysate from HeLa cells at 50 µg
Developed using the ECL technique.
Predicted band size: 66 kDa
Exposure time: 3 minutes
ab117809 at 1 µg/ml staining IGF2BP2 / IMP-2 by WB, following immunoprecipitation of whole cell lysate from 293T cells.
Lane 1; IP using an antibody to an upstream epitope.
Lane 2; IP using ab117809 at 6 µg/mg lysate.
Lane 3; IP using control IgG.
1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised ECL with a 30 second exposure.
ab117809 has not yet been referenced specifically in any publications.