Anti-IGF2BP2 antibody [1E3.01E5] (ab128175)

Overview

  • Product name
    Anti-IGF2BP2 antibody [1E3.01E5]
    See all IGF2BP2 primary antibodies
  • Description
    Mouse monoclonal [1E3.01E5] to IGF2BP2
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human IGF2BP2 protein.

  • Positive control
    • HeLa and Jurkat whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab128175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 66 kDa.
IP Use at 20 µg/mg of lysate.

Target

  • Function
    Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs.
  • Tissue specificity
    Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis.
  • Sequence similarities
    Belongs to the RRM IMP/VICKZ family.
    Contains 4 KH domains.
    Contains 2 RRM (RNA recognition motif) domains.
  • Domain
    The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA.
  • Cellular localization
    Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa.
  • Information by UniProt
  • Database links
  • Alternative names
    • Hepatocellular carcinoma autoantigen p62 antibody
    • IF2B2_HUMAN antibody
    • IGF 2 mRNA binding protein 2 antibody
    • IGF II mRNA binding protein 2 antibody
    • IGF-II mRNA-binding protein 2 antibody
    • IGF2 mRNA-binding protein 2 antibody
    • Igf2bp2 antibody
    • IMP 2 antibody
    • IMP-2 antibody
    • IMP2 antibody
    • Insulin like growth factor 2 mRNA binding protein 2 antibody
    • Insulin-like growth factor 2 mRNA-binding protein 2 antibody
    • OTTHUMP00000082606 antibody
    • OTTHUMP00000082607 antibody
    • OTTHUMP00000082608 antibody
    • p62 antibody
    • VICKZ 2 antibody
    • VICKZ family member 2 antibody
    • VICKZ2 antibody
    see all

Images

  • All lanes : Anti-IGF2BP2 antibody [1E3.01E5] (ab128175) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 66 kDa


    Exposure time: 10 seconds
  • Detection of IGF2BP2 by Western Blot of Immunprecipitate.

    ab128175 at 0.04 µg/ml staining IGF2BP2 in HeLa whole cell lysate immunoprecipitated using ab128175 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab128175 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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To our knowledge, ab128175 has not been tested in RIP. Therefore, I can offer a discount off a future purchase if you buy ab128175 now, test it in RIP and submit feedback to us in the form o...

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