Key features and details
- Mouse monoclonal [1E3.01E5] to IGF2BP2/IMP-2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-IGF2BP2/IMP-2 antibody [1E3.01E5]
See all IGF2BP2/IMP-2 primary antibodies
DescriptionMouse monoclonal [1E3.01E5] to IGF2BP2/IMP-2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human IGF2BP2/IMP-2.
- HeLa and Jurkat whole cell lysates.
Previously labelled as IGF2BP2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
pH 7 to 8
Concentration information loading...
Purification notesab128175 was purified using a Mouse immunoglobulin binding ligand.
Light chain typekappa
Our Abpromise guarantee covers the use of ab128175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 66 kDa.|
|IP||Use at 20 µg/mg of lysate.|
FunctionBinds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs.
Tissue specificityExpressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis.
Sequence similaritiesBelongs to the RRM IMP/VICKZ family.
Contains 4 KH domains.
Contains 2 RRM (RNA recognition motif) domains.
DomainThe third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA.
Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa.
- Information by UniProt
- Hepatocellular carcinoma autoantigen p62 antibody
- IF2B2_HUMAN antibody
- IGF 2 mRNA binding protein 2 antibody
All lanes : Anti-IGF2BP2/IMP-2 antibody [1E3.01E5] (ab128175) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 66 kDa
Exposure time: 10 seconds
Detection of IGF2BP2/IMP-2 by Western Blot of Immunprecipitate.
ab128175 at 0.04 µg/ml staining IGF2BP2/IMP-2 in HeLa whole cell lysate immunoprecipitated using ab128175 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ab128175 has been referenced in 3 publications.
- Li T et al. METTL3 facilitates tumor progression via an m6A-IGF2BP2-dependent mechanism in colorectal carcinoma. Mol Cancer 18:112 (2019). PubMed: 31230592
- Spiniello M et al. HyPR-MS for Multiplexed Discovery of MALAT1, NEAT1, and NORAD lncRNA Protein Interactomes. J Proteome Res 17:3022-3038 (2018). PubMed: 29972301
- Ye S et al. IGF2BP2 promotes colorectal cancer cell proliferation and survival through interfering with RAF-1 degradation by miR-195. FEBS Lett 590:1641-50 (2016). PubMed: 27153315