Anti-IGF2BP2/IMP-2 antibody (ab117809)
Key features and details
- Rabbit polyclonal to IGF2BP2/IMP-2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IGF2BP2/IMP-2 antibody
See all IGF2BP2/IMP-2 primary antibodies -
Description
Rabbit polyclonal to IGF2BP2/IMP-2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla
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Immunogen
Synthetic peptide corresponding to Human IGF2BP2/IMP-2 aa 549-599.
Database link: NP_006539.3 -
Positive control
- Whole cell lysate from 293T, Jurkat and HeLa cells.
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General notes
Previously labelled as IGF2BP2.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab117809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 66 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs. -
Tissue specificity
Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis. -
Sequence similarities
Belongs to the RRM IMP/VICKZ family.
Contains 4 KH domains.
Contains 2 RRM (RNA recognition motif) domains. -
Domain
The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA. -
Cellular localization
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa. - Information by UniProt
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Database links
- Entrez Gene: 10644 Human
- Omim: 608289 Human
- SwissProt: Q9Y6M1 Human
- Unigene: 35354 Human
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Alternative names
- Hepatocellular carcinoma autoantigen p62 antibody
- IF2B2_HUMAN antibody
- IGF 2 mRNA binding protein 2 antibody
see all
Images
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Western blot - Anti-IGF2BP2/IMP-2 antibody (ab117809)All lanes : Anti-IGF2BP2/IMP-2 antibody (ab117809) at 0.1 µg/ml
Lane 1 : Whole cell lysate from 293T cells at 50 µg
Lane 2 : Whole cell lysate from 293T cells at 15 µg
Lane 3 : Whole cell lysate from Jurkat cells at 50 µg
Lane 4 : Whole cell lysate from HeLa cells at 50 µg
Developed using the ECL technique.
Predicted band size: 66 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-IGF2BP2/IMP-2 antibody (ab117809)ab117809 at 1 µg/ml staining IGF2BP2/IMP-2 by WB, following immunoprecipitation of whole cell lysate from 293T cells.
Lane 1; IP using an antibody to an upstream epitope.
Lane 2; IP using ab117809 at 6 µg/mg lysate.
Lane 3; IP using control IgG.
1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised ECL with a 30 second exposure.
Protocols
Datasheets and documents
References (0)
ab117809 has not yet been referenced specifically in any publications.
