Anti-IGF2BP2/IMP-2 antibody (ab117809)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-IGF2BP2/IMP-2 antibody
See all IGF2BP2/IMP-2 primary antibodies -
Description
Rabbit polyclonal to IGF2BP2/IMP-2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla
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Immunogen
Synthetic peptide corresponding to Human IGF2BP2/IMP-2 aa 549-599.
Database link: NP_006539.3 -
Positive control
- Whole cell lysate from 293T, Jurkat and HeLa cells.
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General notes
Previously labelled as IGF2BP2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab117809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 66 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs. -
Tissue specificity
Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis. -
Sequence similarities
Belongs to the RRM IMP/VICKZ family.
Contains 4 KH domains.
Contains 2 RRM (RNA recognition motif) domains. -
Domain
The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA. -
Cellular localization
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa. - Information by UniProt
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Database links
- Entrez Gene: 10644 Human
- Omim: 608289 Human
- SwissProt: Q9Y6M1 Human
- Unigene: 35354 Human
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Alternative names
- Hepatocellular carcinoma autoantigen p62 antibody
- IF2B2_HUMAN antibody
- IGF 2 mRNA binding protein 2 antibody
see all
Images
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Western blot - Anti-IGF2BP2/IMP-2 antibody (ab117809)All lanes : Anti-IGF2BP2/IMP-2 antibody (ab117809) at 0.1 µg/ml
Lane 1 : Whole cell lysate from 293T cells at 50 µg
Lane 2 : Whole cell lysate from 293T cells at 15 µg
Lane 3 : Whole cell lysate from Jurkat cells at 50 µg
Lane 4 : Whole cell lysate from HeLa cells at 50 µg
Developed using the ECL technique.
Predicted band size: 66 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-IGF2BP2/IMP-2 antibody (ab117809)ab117809 at 1 µg/ml staining IGF2BP2/IMP-2 by WB, following immunoprecipitation of whole cell lysate from 293T cells.
Lane 1; IP using an antibody to an upstream epitope.
Lane 2; IP using ab117809 at 6 µg/mg lysate.
Lane 3; IP using control IgG.
1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised ECL with a 30 second exposure.
Datasheets and documents
References
ab117809 has not yet been referenced specifically in any publications.
