Recombinant Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)
![Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)](https://www.abcam.com/ps/products/129/ab129071/Images/ab129071-143015-anti-igf2bp2-imp-2-antibody-epr67402-western-blot.jpg "Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6740(2)] to IGF2BP2/IMP-2
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)]
See all IGF2BP2/IMP-2 primary antibodies -
Description
Rabbit monoclonal [EPR6740(2)] to IGF2BP2/IMP-2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human IGF2BP2/IMP-2 aa 100-200. The exact sequence is proprietary.
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Positive control
- 293T and Caco 2 cell lysates; Human colon tissue.
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General notes
Previously labelled as IGF2BP2.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.00 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6740(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa).
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa). |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.
Target
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Function
Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs. -
Tissue specificity
Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis. -
Sequence similarities
Belongs to the RRM IMP/VICKZ family.
Contains 4 KH domains.
Contains 2 RRM (RNA recognition motif) domains. -
Domain
The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA. -
Cellular localization
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa. - Information by UniProt
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Database links
- Entrez Gene: 10644 Human
- Entrez Gene: 319765 Mouse
- Entrez Gene: 303824 Rat
- Omim: 608289 Human
- SwissProt: Q9Y6M1 Human
- SwissProt: Q5SF07 Mouse
- Unigene: 35354 Human
- Unigene: 489442 Mouse
see all -
Alternative names
- Hepatocellular carcinoma autoantigen p62 antibody
- IF2B2_HUMAN antibody
- IGF 2 mRNA binding protein 2 antibody
see all
Images
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Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)All lanes : Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Caco 2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 66 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)](https://www.abcam.com/ps/products/129/ab129071/Images/ab129071-143014-anti-igf2bp2-imp-2-antibody-epr67402-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)ab129071, at 1/50 dilution, staining IGF2BP2/IMP-2 in paraffin-embedded Human colon tissue by Immunohistochemistry
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)](https://www.abcam.com/ps/products/129/ab129071/Images/ab129071-143013-anti-igf2bp2-imp-2-antibody-epr67402.jpg)
OI-RD Scanning - Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071) -
![Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)](https://www.abcam.com/ps/products/129/ab129071/Images/ab129071-4-benefits-of-recombinant-antibodies.png)
Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab129071 has been referenced in 1 publication.
- McMullen ER et al. CCN6 regulates IGF2BP2 and HMGA2 signaling in metaplastic carcinomas of the breast. Breast Cancer Res Treat 172:577-586 (2018). PubMed: 30220054