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Epigenetics and Nuclear Signaling DNA / RNA Translation Regulation
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RecombinantRabMAb

Recombinant Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)

  • Datasheet
  • SDS
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Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)
  • OI-RD Scanning - Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)
  • Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6740(2)] to IGF2BP2/IMP-2
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Protein
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Recombinant Human IGF2BP2/IMP-2 protein (ab153107)

View more associated products

Overview

  • Product name

    Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)]
    See all IGF2BP2/IMP-2 primary antibodies
  • Description

    Rabbit monoclonal [EPR6740(2)] to IGF2BP2/IMP-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human IGF2BP2/IMP-2 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • 293T and Caco 2 cell lysates; Human colon tissue.
  • General notes

     

     

    Previously labelled as IGF2BP2. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.00 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6740(2)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Signal Transduction
    • Growth Factors/Hormones
    • Insulin / Insulin-like
    • Immunology
    • Immune System Diseases
    • Autoimmune
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Metabolism
    • Types of disease
    • Metabolic disorders

Associated products

  • Alternative Versions

    • Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] - BSA and Azide free (ab248279)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human IGF2BP2/IMP-2 protein (ab153107)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs.
    • Tissue specificity

      Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis.
    • Sequence similarities

      Belongs to the RRM IMP/VICKZ family.
      Contains 4 KH domains.
      Contains 2 RRM (RNA recognition motif) domains.
    • Domain

      The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA.
    • Cellular localization

      Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa.
    • Target information above from: UniProt accession Q9Y6M1 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 10644 Human
      • Entrez Gene: 319765 Mouse
      • Entrez Gene: 303824 Rat
      • Omim: 608289 Human
      • SwissProt: Q9Y6M1 Human
      • SwissProt: Q5SF07 Mouse
      • Unigene: 35354 Human
      • Unigene: 489442 Mouse
      • Unigene: 17318 Rat
      see all
    • Alternative names

      • Hepatocellular carcinoma autoantigen p62 antibody
      • IF2B2_HUMAN antibody
      • IGF 2 mRNA binding protein 2 antibody
      • IGF II mRNA binding protein 2 antibody
      • IGF-II mRNA-binding protein 2 antibody
      • IGF2 mRNA-binding protein 2 antibody
      • Igf2bp2 antibody
      • IMP 2 antibody
      • IMP-2 antibody
      • IMP2 antibody
      • Insulin like growth factor 2 mRNA binding protein 2 antibody
      • Insulin-like growth factor 2 mRNA-binding protein 2 antibody
      • OTTHUMP00000082606 antibody
      • OTTHUMP00000082607 antibody
      • OTTHUMP00000082608 antibody
      • p62 antibody
      • VICKZ 2 antibody
      • VICKZ family member 2 antibody
      • VICKZ2 antibody
      see all

    Images

    • Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)
      Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)
      All lanes : Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Caco 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 66 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)

      ab129071, at 1/50 dilution, staining IGF2BP2/IMP-2 in paraffin-embedded Human colon tissue by Immunohistochemistry

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)
      OI-RD Scanning - Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)
      Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab129071? Please let us know so that we can cite the reference in this datasheet.

    ab129071 has been referenced in 1 publication.

    • McMullen ER  et al. CCN6 regulates IGF2BP2 and HMGA2 signaling in metaplastic carcinomas of the breast. Breast Cancer Res Treat 172:577-586 (2018). PubMed: 30220054

    Customer reviews and Q&As

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