Recombinant
RabMAb

Recombinant Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)

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Overview

  • Product name

    Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)]
    See all IGF2BP2/IMP-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR6740(2)] to IGF2BP2/IMP-2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human IGF2BP2/IMP-2 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • 293T and Caco 2 cell lysates; Human colon tissue.

  • General notes

     

     

    Previously labelled as IGF2BP2. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab129071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    • Function

      Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs.

    • Tissue specificity

      Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis.

    • Sequence similarities

      Belongs to the RRM IMP/VICKZ family.
      Contains 4 KH domains.
      Contains 2 RRM (RNA recognition motif) domains.

    • Domain

      The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA.

    • Cellular localization

      Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Hepatocellular carcinoma autoantigen p62 antibody
      • IF2B2_HUMAN antibody
      • IGF 2 mRNA binding protein 2 antibody
      • IGF II mRNA binding protein 2 antibody
      • IGF-II mRNA-binding protein 2 antibody
      • IGF2 mRNA-binding protein 2 antibody
      • Igf2bp2 antibody
      • IMP 2 antibody
      • IMP-2 antibody
      • IMP2 antibody
      • Insulin like growth factor 2 mRNA binding protein 2 antibody
      • Insulin-like growth factor 2 mRNA-binding protein 2 antibody
      • OTTHUMP00000082606 antibody
      • OTTHUMP00000082607 antibody
      • OTTHUMP00000082608 antibody
      • p62 antibody
      • VICKZ 2 antibody
      • VICKZ family member 2 antibody
      • VICKZ2 antibody
      see all

    Images

    • Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)
      Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)
      All lanes : Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Caco 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 66 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)

      ab129071, at 1/50 dilution, staining IGF2BP2/IMP-2 in paraffin-embedded Human colon tissue by Immunohistochemistry

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Other - Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)
      Other - Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • McMullen ER  et al. CCN6 regulates IGF2BP2 and HMGA2 signaling in metaplastic carcinomas of the breast. Breast Cancer Res Treat 172:577-586 (2018). Read more (PubMed: 30220054) »
    See 1 Publication for this product

    Publishing research using ab129071? Please let us know so that we can cite the reference in this datasheet.

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