Product nameAnti-IGF2BP2/IMP-2 antibody [EPR6740(2)]
See all IGF2BP2/IMP-2 primary antibodies
DescriptionRabbit monoclonal [EPR6740(2)] to IGF2BP2/IMP-2
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human IGF2BP2/IMP-2 aa 100-200. The exact sequence is proprietary.
- 293T and Caco 2 cell lysates; Human colon tissue.
Previously labelled as IGF2BP2.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.00 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionBinds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs.
Tissue specificityExpressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis.
Sequence similaritiesBelongs to the RRM IMP/VICKZ family.
Contains 4 KH domains.
Contains 2 RRM (RNA recognition motif) domains.
DomainThe third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA.
Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa.
- Information by UniProt
- Hepatocellular carcinoma autoantigen p62 antibody
- IF2B2_HUMAN antibody
- IGF 2 mRNA binding protein 2 antibody
All lanes : Anti-IGF2BP2/IMP-2 antibody [EPR6740(2)] (ab129071) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Caco 2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 66 kDa
ab129071, at 1/50 dilution, staining IGF2BP2/IMP-2 in paraffin-embedded Human colon tissue by Immunohistochemistry
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
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