Recombinant
RabMAb

Recombinant Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)

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Overview

  • Product name

    Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)]
    See all IGF2BP2/IMP-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR6741(B)] to IGF2BP2/IMP-2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human IGF2BP2/IMP-2.

  • Positive control

    • 293T, Caco2, HepG2, HeLa and SH SY5Y cell lysates, rat brain and mouse heart lysate; Human colon and human lung carcinoma tissue.

  • General notes

    The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.

    Previously labelled as IGF2BP2. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab124930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Detects a band of approximately 55-65 kDa (predicted molecular weight: 66 kDa).
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
ICC/IF 1/100 - 1/250.

Target

  • Function

    Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs.

  • Tissue specificity

    Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis.

  • Sequence similarities

    Belongs to the RRM IMP/VICKZ family.
    Contains 4 KH domains.
    Contains 2 RRM (RNA recognition motif) domains.

  • Domain

    The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA.

  • Cellular localization

    Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Hepatocellular carcinoma autoantigen p62 antibody
    • IF2B2_HUMAN antibody
    • IGF 2 mRNA binding protein 2 antibody
    • IGF II mRNA binding protein 2 antibody
    • IGF-II mRNA-binding protein 2 antibody
    • IGF2 mRNA-binding protein 2 antibody
    • Igf2bp2 antibody
    • IMP 2 antibody
    • IMP-2 antibody
    • IMP2 antibody
    • Insulin like growth factor 2 mRNA binding protein 2 antibody
    • Insulin-like growth factor 2 mRNA-binding protein 2 antibody
    • OTTHUMP00000082606 antibody
    • OTTHUMP00000082607 antibody
    • OTTHUMP00000082608 antibody
    • p62 antibody
    • VICKZ 2 antibody
    • VICKZ family member 2 antibody
    • VICKZ2 antibody
    see all

Images

  • Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)
    Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)
    All lanes : Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930) at 1/5000 dilution

    Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 66 kDa
    Observed band size: 60,62 kDa
    why is the actual band size different from the predicted?


    Exposure time: 60 seconds


    Blocking buffer and concentration 5% NFDM/TBST
    Diluting buffer and concentration 5% NFDM/TBST

    The double bands are consistent with what has been described in PMID 23177649.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling IGF2BP2/IMP-2 with purified ab124930 at 1/200 dilution (4.8 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunocytochemistry/ Immunofluorescence - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)
    Immunocytochemistry/ Immunofluorescence - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)

    Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling IGF2BP2/IMP-2 with purified ab124930 at 1/250 dilution (3.8μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889, an anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml). ab150077, a Goat anti-rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)
    Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)
    Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930) at 1/10000 dilution (purified) + Mouse heart lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 66 kDa
    Observed band size: 66 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)
    Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)
    All lanes : Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930) at 1/2000 dilution (purified)

    Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 66 kDa
    Observed band size: 66 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)

    ab124930 (unpurified), at 1/50 dilution, staining IGF2BP2/IMP-2 in formalin-fixed, paraffin-embedded Human colon tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Other - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)
    Other - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:

  • Wu L  et al. miR-181a-5p suppresses invasion and migration of HTR-8/SVneo cells by directly targeting IGF2BP2. Cell Death Dis 9:16 (2018). WB . Read more (PubMed: 29339719) »
  • He X  et al. IGF2BP2 Overexpression Indicates Poor Survival in Patients with Acute Myelocytic Leukemia. Cell Physiol Biochem 51:1945-1956 (2018). Read more (PubMed: 30513526) »
See all 3 Publications for this product

Publishing research using ab124930? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IGF2BP2 / IMP-2 antibody [EPR6741(B)]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human lung carcinoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mm Tris/1mm EDTA, PH 9.0
Permeabilization
No
Specification
human lung carcinoma
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Read More

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Verified customer

Submitted Feb 19 2018

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