Recombinant Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)
![Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)](https://www.abcam.com/ps/products/124/ab124930/Images/ab124930-344317-antiIGF2BP2IMP2antibodyEPR6741B-westernblot-human.jpg "Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6741(B)] to IGF2BP2/IMP-2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)]
See all IGF2BP2/IMP-2 primary antibodies -
Description
Rabbit monoclonal [EPR6741(B)] to IGF2BP2/IMP-2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human IGF2BP2/IMP-2.
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Positive control
- 293T, Caco2, HepG2, HeLa and SH SY5Y cell lysates, rat brain and mouse heart lysate; Human colon and human lung carcinoma tissue.
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General notes
The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.00 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6741(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124930 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000 - 1/5000. Detects a band of approximately 55-65 kDa (predicted molecular weight: 66 kDa).
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| IHC-P | (1) |
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/250.
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| Notes |
|---|
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WB
1/2000 - 1/5000. Detects a band of approximately 55-65 kDa (predicted molecular weight: 66 kDa). |
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IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/250. |
Target
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Function
Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs. -
Tissue specificity
Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis. -
Sequence similarities
Belongs to the RRM IMP/VICKZ family.
Contains 4 KH domains.
Contains 2 RRM (RNA recognition motif) domains. -
Domain
The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA. -
Cellular localization
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa. - Information by UniProt
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Database links
- Entrez Gene: 10644 Human
- Entrez Gene: 319765 Mouse
- Entrez Gene: 303824 Rat
- Omim: 608289 Human
- SwissProt: Q9Y6M1 Human
- SwissProt: Q5SF07 Mouse
- Unigene: 35354 Human
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Alternative names
- Hepatocellular carcinoma autoantigen p62 antibody
- IF2B2_HUMAN antibody
- IGF 2 mRNA binding protein 2 antibody
see all
Images
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Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)All lanes : Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930) at 1/5000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 60,62 kDa why is the actual band size different from the predicted?
Exposure time: 60 secondsBlocking buffer and concentration 5% NFDM/TBST
Diluting buffer and concentration 5% NFDM/TBSTThe double bands are consistent with what has been described in PMID 23177649.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)](https://www.abcam.com/ps/products/124/ab124930/Images/ab124930-274275-anti-igf2bp2-imp-2-antibody-epr6741b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling IGF2BP2/IMP-2 with purified ab124930 at 1/200 dilution (4.8 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
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![Immunocytochemistry/ Immunofluorescence - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)](https://www.abcam.com/ps/products/124/ab124930/Images/ab124930-274276-anti-igf2bp2-imp-2-antibody-epr6741b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling IGF2BP2/IMP-2 with purified ab124930 at 1/250 dilution (3.8μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889, an anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml). ab150077, a Goat anti-rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)](https://www.abcam.com/ps/products/124/ab124930/Images/ab124930-274274-anti-igf2bp2-imp-2-antibody-epr6741b-western-blot.jpg)
Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930) at 1/10000 dilution (purified) + Mouse heart lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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![Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)](https://www.abcam.com/ps/products/124/ab124930/Images/ab124930-274273-anti-igf2bp2-imp-2-antibody-epr6741b-western-blot.jpg)
Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)All lanes : Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930) at 1/2000 dilution (purified)
Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)](https://www.abcam.com/ps/products/124/ab124930/Images/ab124930-138955-anti-igf2bp2-imp-2-antibody-epr6741b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)ab124930 (unpurified), at 1/50 dilution, staining IGF2BP2/IMP-2 in formalin-fixed, paraffin-embedded Human colon tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)](https://www.abcam.com/ps/products/124/ab124930/Images/ab124930-138954-anti-igf2bp2-imp-2-antibody-epr6741b.jpg)
OI-RD Scanning - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930) -
![Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)](https://www.abcam.com/ps/products/124/ab124930/Images/ab124930-9-benefits-of-recombinant-antibodies.png)
Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (ab124930)
Protocols
Datasheets and documents
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Datasheet download
References (9)
ab124930 has been referenced in 9 publications.
- Sekulovski N et al. Niclosamide's potential direct targets in ovarian cancer†. Biol Reprod 105:403-412 (2021). PubMed: 33855343
- Ma YS et al. microRNA-320b suppresses HNF4G and IGF2BP2 expression to inhibit angiogenesis and tumor growth of lung cancer. Carcinogenesis 42:762-771 (2021). PubMed: 33758932
- Gao T et al. Long non-coding RNA 91H regulates IGF2 expression by interacting with IGF2BP2 and promotes tumorigenesis in colorectal cancer. Artif Cells Nanomed Biotechnol 48:664-671 (2020). PubMed: 32070145
- Wan BS et al. Insulin-like growth factor 2 mRNA-binding protein 1 promotes cell proliferation via activation of AKT and is directly targeted by microRNA-494 in pancreatic cancer. World J Gastroenterol 25:6063-6076 (2019). PubMed: 31686763
- Wang Y et al. LncRNA LINRIS stabilizes IGF2BP2 and promotes the aerobic glycolysis in colorectal cancer. Mol Cancer 18:174 (2019). PubMed: 31791342
- Wu L et al. miR-181a-5p suppresses invasion and migration of HTR-8/SVneo cells by directly targeting IGF2BP2. Cell Death Dis 9:16 (2018). WB . PubMed: 29339719
- He X et al. IGF2BP2 Overexpression Indicates Poor Survival in Patients with Acute Myelocytic Leukemia. Cell Physiol Biochem 51:1945-1956 (2018). PubMed: 30513526
- Ye S et al. IGF2BP2 promotes colorectal cancer cell proliferation and survival through interfering with RAF-1 degradation by miR-195. FEBS Lett 590:1641-50 (2016). PubMed: 27153315
- Lin W et al. Dynamic epigenetic regulation by menin during pancreatic islet tumor formation. Mol Cancer Res 13:689-98 (2015). PubMed: 25537453
