Anti-IGFBP1 antibody (ab228741)

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Overview

  • Product name

    Anti-IGFBP1 antibody
    See all IGFBP1 primary antibodies

  • Description

    Rabbit polyclonal to IGFBP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human IGFBP1 (internal sequence). The exact sequence is proprietary.
    Database link: P08833

  • Positive control

    • WB: HepG2 whole cell extract. IP: HepG2 whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab228741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 28 kDa.
IP 1/100 - 1/500.

Target

  • Function

    IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.

  • Sequence similarities

    Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.

  • Post-translational
    modifications

    Phosphorylated; probably by casein kinase II. Alters the affinity of the protein for IGFs.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • AFBP antibody
      • Alpha pregnancy associated endometrial globulin antibody
      • Amniotic fluid binding protein antibody
      • Binding protein 25 antibody
      • Binding protein 26 antibody
      • Binding protein 28 antibody
      • Growth hormone independent binding protein antibody
      • hIGFBP 1 antibody
      • hIGFBP1 antibody
      • IBP 1 antibody
      • IBP-1 antibody
      • IBP1 antibody
      • IBP1_HUMAN antibody
      • IGF binding protein 1 antibody
      • IGF BP25 antibody
      • IGF-binding protein 1 antibody
      • IGFBP 1 antibody
      • IGFBP-1 antibody
      • IGFBP1 antibody
      • Insulin like growth factor binding protein 1 antibody
      • Insulin-like growth factor-binding protein 1 antibody
      • Placental protein 12 antibody
      • PP 12 antibody
      • PP12 antibody
      see all

    Images

    • Western blot - Anti-IGFBP1 antibody (ab228741)
      Western blot - Anti-IGFBP1 antibody (ab228741)
      Anti-IGFBP1 antibody (ab228741) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

      Predicted band size: 28 kDa



      12% SDS-PAGE gel.

    • Immunoprecipitation - Anti-IGFBP1 antibody (ab228741)
      Immunoprecipitation - Anti-IGFBP1 antibody (ab228741)

      IGFBP1 was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract with 5 µg of ab228741. Western blot was performed from the immunoprecipitate using ab228741.

      Lane 1: HepG2 whole cell extract (Input). 
      Lane 2: Control IgG instead of ab228741 in HepG2 whole cell extract.
      Lane 3: ab228741 IP in HepG2 whole cell extract.

    References

    ab228741 has not yet been referenced specifically in any publications.

    Publishing research using ab228741? Please let us know so that we can cite the reference in this datasheet.

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