Anti-IGFBP1 antibody (ab228741)
Key features and details
- Rabbit polyclonal to IGFBP1
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-IGFBP1 antibody
See all IGFBP1 primary antibodies -
Description
Rabbit polyclonal to IGFBP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human IGFBP1 (internal sequence). The exact sequence is proprietary.
Database link: P08833 -
Positive control
- WB: HepG2 whole cell extract. IP: HepG2 whole cell extract. ICC/IF: HepG2 cells.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab228741 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 28 kDa. | |
| IP | 1/100 - 1/500. | |
| ICC/IF | 1/100 - 1/1000. |
Target
-
Function
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. -
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
Post-translational
modificationsPhosphorylated; probably by casein kinase II. Alters the affinity of the protein for IGFs. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 3484 Human
- Omim: 146730 Human
- SwissProt: P08833 Human
- Unigene: 642938 Human
-
Alternative names
- AFBP antibody
- Alpha pregnancy associated endometrial globulin antibody
- Amniotic fluid binding protein antibody
see all
Images
-
Western blot - Anti-IGFBP1 antibody (ab228741)Anti-IGFBP1 antibody (ab228741) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg
Predicted band size: 28 kDa12% SDS-PAGE gel.
-
Immunoprecipitation - Anti-IGFBP1 antibody (ab228741)IGFBP1 was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract with 5 µg of ab228741. Western blot was performed from the immunoprecipitate using ab228741.
Lane 1: HepG2 whole cell extract (Input).
Lane 2: Control IgG instead of ab228741 in HepG2 whole cell extract.
Lane 3: ab228741 IP in HepG2 whole cell extract. -
Immunocytochemistry/ Immunofluorescence - Anti-IGFBP1 antibody (ab228741)HepG2 cells stained for IGFBP1 (green) using ab228741 at 1/500 dilution in ICC/IF.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab228741 has not yet been referenced specifically in any publications.
