Recombinant
RabMAb

Anti-IGFBP1 antibody [EPR14472(B)] (ab181141)

Overview

  • Product name
    Anti-IGFBP1 antibody [EPR14472(B)]
    See all IGFBP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR14472(B)] to IGFBP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human IGFBP1 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: P08833

  • Positive control
    • Human placenta lysate; HepG2 cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).

Target

  • Function
    IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.
  • Sequence similarities
    Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • Post-translational
    modifications
    Phosphorylated; probably by casein kinase II. Alters the affinity of the protein for IGFs.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • AFBP antibody
    • Alpha pregnancy associated endometrial globulin antibody
    • Amniotic fluid binding protein antibody
    • Binding protein 25 antibody
    • Binding protein 26 antibody
    • Binding protein 28 antibody
    • Growth hormone independent binding protein antibody
    • hIGFBP 1 antibody
    • hIGFBP1 antibody
    • IBP 1 antibody
    • IBP-1 antibody
    • IBP1 antibody
    • IBP1_HUMAN antibody
    • IGF binding protein 1 antibody
    • IGF BP25 antibody
    • IGF-binding protein 1 antibody
    • IGFBP 1 antibody
    • IGFBP-1 antibody
    • IGFBP1 antibody
    • Insulin like growth factor binding protein 1 antibody
    • Insulin-like growth factor-binding protein 1 antibody
    • Placental protein 12 antibody
    • PP 12 antibody
    • PP12 antibody
    see all

Images

  • Anti-IGFBP1 antibody [EPR14472(B)] (ab181141) at 1/1000 dilution + HepG2 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 28 kDa
    Observed band size: 30 kDa (why is the actual band size different from the predicted?)

  • Anti-IGFBP1 antibody [EPR14472(B)] (ab181141) at 1/5000 dilution + Human placenta lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 28 kDa
    Observed band size: 30 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Charni-Natan M  et al. Various stress stimuli rewire the profile of liver secretome in a p53-dependent manner. Cell Death Dis 9:647 (2018). Read more (PubMed: 29844359) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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