Key features and details
- Rabbit polyclonal to IGFBP2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IGFBP2 antibody
See all IGFBP2 primary antibodies
DescriptionRabbit polyclonal to IGFBP2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Pig
Recombinant fragment within Human IGFBP2 (internal sequence). The exact sequence is proprietary.
Database link: P18065
- ICC/IF: Huh-7 cells. IHC-P: Human breast carcinoma tissue. WB: SK-N-AS whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 35 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionInhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Sequence similaritiesContains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain.
DomainThe C-terminus is required for IGF-binding and growth inhibition.
- Information by UniProt
- BP 2 antibody
- BP2 antibody
- IBP 2 antibody
Anti-IGFBP2 antibody (ab227881) at 1/1000 dilution + SK-N-AS whole cell lysate at 30 µg
Predicted band size: 35 kDa
10 % SDS-PAGE gel.
Huh-7 cells stained for IGFBP2 (green) using ab227881 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Blue: Hoechst 33342 staining.
Paraffin-embedded human breast carcinoma tissue stained for IGFBP2 using ab227881 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227881 has been referenced in 1 publication.
- Brockway HM et al. Unique transcriptomic landscapes identified in idiopathic spontaneous and infection related preterm births compared to normal term births. PLoS One 14:e0225062 (2019). PubMed: 31703110