- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-IGFBP2 antibody
See all IGFBP2 primary antibodies -
DescriptionRabbit polyclonal to IGFBP2
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Host speciesRabbit
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Tested applicationsSuitable for: Flow Cyt, ICC/IF, IHC-P, WB, ELISAmore details
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Species reactivityReacts with: Human
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Immunogen
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Positive control
- Purchase matching WB positive control:Recombinant human IGFBP2 protein
- WB: Jurkat cell lysate IHC-P: Human brain and lung tissue ICC/IF: A549 cells FC: Jurkat cells
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab91404 is purified through a protein A column, followed by peptide affinity purification.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab91404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/10 - 1/50. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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| ICC/IF | 1/10 - 1/50. | |
| IHC-P | 1/10 - 1/50. | |
| WB | 1/1000. Predicted molecular weight: 35 kDa. | |
| ELISA | 1/1000. |
Target
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FunctionInhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
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Sequence similaritiesContains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
DomainThe C-terminus is required for IGF-binding and growth inhibition.
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Post-translational
modificationsO-glycosylated. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 3485 Human
- Omim: 146731 Human
- SwissProt: P18065 Human
- Unigene: 438102 Human
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Alternative names
- BP 2 antibody
- BP2 antibody
- IBP 2 antibody
see all
Images
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Western blot - Anti-IGFBP2 antibody (ab91404)Anti-IGFBP2 antibody (ab91404) at 2 µg/ml + Jurkat cell lysate at 35 µg
Predicted band size: 35 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody (ab91404)Formalin-fixed and paraffin-embedded human brain tissue with ab91404, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. -
Immunocytochemistry/ Immunofluorescence - Anti-IGFBP2 antibody (ab91404)Confocal immunofluorescent analysis of ab91404 with A549 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). -
Flow Cytometry - Anti-IGFBP2 antibody (ab91404)Flow cytometric analysis of jurkat cells using ab91404 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protocols
References
This product has been referenced in:
- Marthandan S et al. Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts. Biomed Res Int 2015:731938 (2015). WB . Read more (PubMed: 26339636) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"