Anti-IGFBP2 antibody (ab91404)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-IGFBP2 antibody
See all IGFBP2 primary antibodies -
Description
Rabbit polyclonal to IGFBP2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, ICC/IF, IHC-P, WB, ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
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Positive control
- Purchase matching WB positive control:Recombinant human IGFBP2 protein
- WB: Jurkat whole cell lysate (ab7899) IHC-P: Human brain and lung tissue ICC/IF: A549 cells FC: Jurkat cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab91404 is purified through a protein A column, followed by peptide affinity purification. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab91404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt | 1/10 - 1/50. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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ICC/IF | 1/10 - 1/50. | |
IHC-P | 1/10 - 1/50. | |
WB | 1/1000. Predicted molecular weight: 35 kDa. | |
ELISA | 1/1000. |
Target
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Function
Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
Domain
The C-terminus is required for IGF-binding and growth inhibition. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3485 Human
- Omim: 146731 Human
- SwissProt: P18065 Human
- Unigene: 438102 Human
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Alternative names
- BP 2 antibody
- BP2 antibody
- IBP 2 antibody
see all
Images
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Anti-IGFBP2 antibody (ab91404) at 2 µg/ml + Jurkat cell lysate at 35 µg
Predicted band size: 35 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody (ab91404)Formalin-fixed and paraffin-embedded human brain tissue with ab91404, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Confocal immunofluorescent analysis of ab91404 with A549 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Flow cytometric analysis of jurkat cells using ab91404 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Marthandan S et al. Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts. Biomed Res Int 2015:731938 (2015). WB . Read more (PubMed: 26339636) »