Recombinant Anti-IGFBP2 antibody [EPR18012-257] (ab188200)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18012-257] to IGFBP2
- Suitable for: IHC-Fr, IP, WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-IGFBP2 antibody [EPR18012-257]
See all IGFBP2 primary antibodies -
Description
Rabbit monoclonal [EPR18012-257] to IGFBP2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, IP, WB, IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human, rat and mouse serum; human liver lysate; T-47D and RAW 264.7 whole cell lysate. IHC-P: Mouse choroid plexus and liver tissue. IHC-Fr: Mouse and rat brain (choroid plexus). Flow Cyt (intra): T-47D and RAW 264.7 cells. IP: Human serum.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18012-257 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry reagents
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr | (1) |
1/500.
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IP |
1/30.
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WB |
1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This antibody is not suitable for human and rat species in IHC application due to non-specific or negative staining. |
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Flow Cyt (Intra) |
1/60.
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Notes |
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IHC-Fr
1/500. |
IP
1/30. |
WB
1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. This antibody is not suitable for human and rat species in IHC application due to non-specific or negative staining. |
Flow Cyt (Intra)
1/60. |
Target
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Function
Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
Domain
The C-terminus is required for IGF-binding and growth inhibition. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3485 Human
- Entrez Gene: 16008 Mouse
- Entrez Gene: 25662 Rat
- Omim: 146731 Human
- SwissProt: P18065 Human
- SwissProt: P47877 Mouse
- SwissProt: Q91VK7 Mouse
- SwissProt: P12843 Rat
see all -
Alternative names
- BP 2 antibody
- BP2 antibody
- IBP 2 antibody
see all
Images
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All lanes : Anti-IGFBP2 antibody [EPR18012-257] (ab188200) at 1/1000 dilution
Lane 1 : Human serum
Lane 2 : Human liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 3 minutesBlocking: 5% NFDM/TBST.
The band in lane 1 is human IgG heavy chain which is often observed in serum and plasma samples.
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Immunohistochemical analysis of paraffin-embedded mouse choroid plexus tissue labeling IGFBP2 with ab188200 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on mouse choroid plexus (PMID: 7525264; PMID: 7678219) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of 4% PFA fixed, 0.2% TritonX-100 permeabilized mouse brain (choroid plexus) tissue labeling IGFBP2 with ab188200 at 1/500 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution. Cytoplasmic staining in the epithelial cells of choroid plexus on mouse tissue section is observed. Counter stained with DAPI.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Perform heat mediated antigen retrieval using Tris-EDTA (pH 9.0) (ab94681). -
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cell line labeling IGFBP2 with ab188200 at 1/60 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
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IGFBP2 was immunoprecipitated from 0.35 mg of human serum with ab188200 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188200 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Human serum 10 μg (Input).
Lane 2: ab188200 IP in Human serum (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab188200 in human serum (-).Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
The band in lane 1 is human IgG heavy chain which is often observed in serum and plasma samples.
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All lanes : Anti-IGFBP2 antibody [EPR18012-257] (ab188200) at 1/1000 dilution
Lane 1 : Mouse serum
Lane 2 : T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate
Lane 3 : Rat serum
Lane 4 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 3 minutesBlocking: 5% NFDM/TBST.
The expression profile observed on T-47D is consistent with what has been described in the literature (PMID: 23515291).
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Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling IGFBP2 with ab188200 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on mouse liver (PMID: 7678219) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of 4% PFA fixed, 0.2% TritonX-100 permeabilized rat brain (choroid plexus) tissue labeling IGFBP2 with ab188200 at 1/500 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution. Cytoplasmic staining in the epithelial cells of choroid plexus on rat tissue section is observed. Counter stained with DAPI.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Perform heat mediated antigen retrieval using Tris-EDTA (pH 9.0) (ab94681). -
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cell line labeling IGFBP2 with ab188200 at 1/60 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab188200 has been referenced in 2 publications.
- Li H et al. IGFBP2 function as a novel biomarker for active lupus nephritis. J Mol Med (Berl) 100:1479-1491 (2022). PubMed: 36008635
- Komata Y et al. Screening of novel Midkine binding protein by BioID2-based proximity labeling. Nagoya J Med Sci 83:495-508 (2021). PubMed: 34552285