Name: Anti-IGFBP2 antibody [EPR18012-257]
Brand: RabMAb
SKU: ab188200
Rating: 4 (1 reviews)

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Western blot - Anti-IGFBP2 antibody [EPR18012-257] (ab188200)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18012-257] to IGFBP2
Suitable for: IHC-Fr, IP, WB, IHC-P, Flow Cyt (Intra)
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-IGFBP2 antibody [EPR18012-257]
See all IGFBP2 primary antibodies
Description

Rabbit monoclonal [EPR18012-257] to IGFBP2
Host species

Rabbit
Tested applications

Suitable for: IHC-Fr, IP, WB, IHC-P, Flow Cyt (Intra)more details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human, rat and mouse serum; human liver lysate; T-47D and RAW 264.7 whole cell lysate. IHC-P: Mouse choroid plexus and liver tissue. IHC-Fr: Mouse and rat brain (choroid plexus). Flow Cyt (intra): T-47D and RAW 264.7 cells. IP: Human serum.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18012-257
Isotype

IgG
Research areas

Alternative Versions
- Anti-IGFBP2 antibody [EPR18012-257] - BSA and Azide free (ab225763)
Compatible Secondaries
Conjugation kits
- Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
Immunohistochemistry reagents
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant human IGFBP2 protein (ab63223)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab188200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-Fr	(1)	1/500.
IP		1/30.
WB		1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P		1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. This antibody is not suitable for human and rat species in IHC application due to non-specific or negative staining.
Flow Cyt (Intra)		1/60.

Notes
IHC-Fr 1/500.
IP 1/30.
WB 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. This antibody is not suitable for human and rat species in IHC application due to non-specific or negative staining.
Flow Cyt (Intra) 1/60.

Function

Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Sequence similarities

Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain.
Domain

The C-terminus is required for IGF-binding and growth inhibition.
Post-translational
modifications

O-glycosylated.
Cellular localization

Secreted.
Target information above from: UniProt accession P18065 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 3485 Human
- Entrez Gene: 16008 Mouse
- Entrez Gene: 25662 Rat
- Omim: 146731 Human
- SwissProt: P18065 Human
- SwissProt: P47877 Mouse
- SwissProt: Q91VK7 Mouse
- SwissProt: P12843 Rat
- Unigene: 438102 Human
- Unigene: 141936 Mouse
- Unigene: 6813 Rat
see all
Alternative names
- BP 2 antibody
- BP2 antibody
- IBP 2 antibody
- IBP-2 antibody
- IBP2 antibody
- IBP2_HUMAN antibody
- IGF binding protein 2 antibody
- IGF BP53 antibody
- IGF-binding protein 2 antibody
- IGFBP 2 antibody
- IGFBP-2 antibody
- IGFBP2 antibody
- IGFBP53 antibody
- Insulin like growth factor binding protein 2 36kDa antibody
- Insulin like growth factor binding protein 2 antibody
- Insulin like growth factor-binding protein 2 precursor antibody
- Insulin-like growth factor-binding protein 2 antibody
see all

Western blot - Anti-IGFBP2 antibody [EPR18012-257] (ab188200)

All lanes : Anti-IGFBP2 antibody [EPR18012-257] (ab188200) at 1/1000 dilution

Lane 1 : Human serum
Lane 2 : Human liver lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 33 kDa
Observed band size: 33 kDa

Exposure time: 3 minutes

Blocking: 5% NFDM/TBST.

The band in lane 1 is human IgG heavy chain which is often observed in serum and plasma samples.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR18012-257] (ab188200)

Immunohistochemical analysis of paraffin-embedded mouse choroid plexus tissue labeling IGFBP2 with ab188200 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on mouse choroid plexus (PMID: 7525264; PMID: 7678219) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Frozen sections) - Anti-IGFBP2 antibody [EPR18012-257] (ab188200)

Immunohistochemical analysis of 4% PFA fixed, 0.2% TritonX-100 permeabilized mouse brain (choroid plexus) tissue labeling IGFBP2 with ab188200 at 1/500 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution. Cytoplasmic staining in the epithelial cells of choroid plexus on mouse tissue section is observed. Counter stained with DAPI.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Perform heat mediated antigen retrieval using Tris-EDTA (pH 9.0) (ab94681).
Flow Cytometry (Intracellular) - Anti-IGFBP2 antibody [EPR18012-257] (ab188200)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cell line labeling IGFBP2 with ab188200 at 1/60 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-IGFBP2 antibody [EPR18012-257] (ab188200)

IGFBP2 was immunoprecipitated from 0.35 mg of human serum with ab188200 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188200 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Human serum 10 μg (Input).
Lane 2: ab188200 IP in Human serum (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab188200 in human serum (-).

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.

The band in lane 1 is human IgG heavy chain which is often observed in serum and plasma samples.
Western blot - Anti-IGFBP2 antibody [EPR18012-257] (ab188200)

All lanes : Anti-IGFBP2 antibody [EPR18012-257] (ab188200) at 1/1000 dilution

Lane 1 : Mouse serum
Lane 2 : T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate
Lane 3 : Rat serum
Lane 4 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 33 kDa
Observed band size: 33 kDa

Exposure time: 3 minutes

Blocking: 5% NFDM/TBST.

The expression profile observed on T-47D is consistent with what has been described in the literature (PMID: 23515291).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP2 antibody [EPR18012-257] (ab188200)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling IGFBP2 with ab188200 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on mouse liver (PMID: 7678219) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Frozen sections) - Anti-IGFBP2 antibody [EPR18012-257] (ab188200)

Immunohistochemical analysis of 4% PFA fixed, 0.2% TritonX-100 permeabilized rat brain (choroid plexus) tissue labeling IGFBP2 with ab188200 at 1/500 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution. Cytoplasmic staining in the epithelial cells of choroid plexus on rat tissue section is observed. Counter stained with DAPI.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Perform heat mediated antigen retrieval using Tris-EDTA (pH 9.0) (ab94681).
Flow Cytometry (Intracellular) - Anti-IGFBP2 antibody [EPR18012-257] (ab188200)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cell line labeling IGFBP2 with ab188200 at 1/60 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Anti-IGFBP2 antibody [EPR18012-257] (ab188200)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab188200? Please let us know so that we can cite the reference in this datasheet.

ab188200 has not yet been referenced specifically in any publications.

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Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Testis, postnatal day 6)

Permeabilization

Yes - 0.1% Triton X-100 in PBS

Specification

Testis, postnatal day 6

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C

Fixative

Paraformaldehyde

MR. Bryan Niedenberger

Verified customer

Submitted Feb 12 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Conjugation kits

Immunohistochemistry reagents

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Domain

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

Certificate of Compliance

References (0)

Customer reviews and Q&As

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Immunohistochemistry (Frozen sections) abreview for Anti-IGFBP2 antibody [EPR18012-257]

Post-translational
modifications