Overview

  • Product name

    Anti-IGFBP2 antibody [EPR3380(2)]
    See all IGFBP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR3380(2)] to IGFBP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human IGFBP2 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human kidney tissue, Human endometrial adenocarcinoma tissue. T47-D, SH-SY5Y, BxPC-3, A549 and NCCIT cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 35 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
    • Sequence similarities

      Contains 1 IGFBP N-terminal domain.
      Contains 1 thyroglobulin type-1 domain.
    • Domain

      The C-terminus is required for IGF-binding and growth inhibition.
    • Post-translational
      modifications

      O-glycosylated.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • BP 2 antibody
      • BP2 antibody
      • IBP 2 antibody
      • IBP-2 antibody
      • IBP2 antibody
      • IBP2_HUMAN antibody
      • IGF binding protein 2 antibody
      • IGF BP53 antibody
      • IGF-binding protein 2 antibody
      • IGFBP 2 antibody
      • IGFBP-2 antibody
      • IGFBP2 antibody
      • IGFBP53 antibody
      • Insulin like growth factor binding protein 2 36kDa antibody
      • Insulin like growth factor binding protein 2 antibody
      • Insulin like growth factor-binding protein 2 precursor antibody
      • Insulin-like growth factor-binding protein 2 antibody
      see all

    Images

    • All lanes : Anti-IGFBP2 antibody [EPR3380(2)] (ab109284) at 1/10000 dilution

      Lane 1 : T47-D cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : BxPC-3 cell lysate
      Lane 4 : A549 cell lysate
      Lane 5 : NCCIT cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 35 kDa

    • Immunohistochemical analysis of paraffin-embedded kidney tissue using 1/100 ab109284.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded endometrial adenocarcinoma tissue with ab109284 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Gao C  et al. Adeno-associated virus type 2-mediated gene transfer of a short hairpin-RNA targeting human IGFBP-2 suppresses the proliferation and invasion of MDA-MB-468 cells. Mol Med Rep 17:4383-4391 (2018). Read more (PubMed: 29344663) »
    • Strub T  et al. SIRT6 haploinsufficiency induces BRAFV600E melanoma cell resistance to MAPK inhibitors via IGF signalling. Nat Commun 9:3440 (2018). Read more (PubMed: 30143629) »
    See all 5 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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