Key features and details
- Rabbit polyclonal to IGFBP3
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-IGFBP3 antibody
See all IGFBP3 primary antibodies
DescriptionRabbit polyclonal to IGFBP3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Human
Synthetic peptide within Human IGFBP3 aa 240-296 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P17936
- Rat brain tissue; Rat kidney and brain lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab217205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 32 kDa).|
|IHC-P||1/100 - 1/500.
If a fluorescent secondary antibody is used the recommended dilution is 1/50 - 1/200.
FunctionIGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Tissue specificityExpressed by most tissues. Present in plasma.
Sequence similaritiesContains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain.
Developmental stageIGFBP3 levels are higher during extrauterine life and peak during puberty.
DomainThe thyroglobulin type-1 domain mediates interaction with HN.
modificationsPhosphorylation sites are present in the extracelllular medium.
- Information by UniProt
- Acid stable subunit of the 140 K IGF complex antibody
- Binding protein 29 antibody
- Binding protein 53 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling IGFBP3 with ab217205 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling IGFBP3 with ab217205 at 1/200 dilution overnight at 4°C, followed by Goat Anti-Rabbit IgG, Cy3 conjugated antibody at 1/200 dilution for 40 minutes at 37°C.
All lanes : Anti-IGFBP3 antibody (ab217205) at 1/200 dilution
Lane 1 : Rat kidney lysate
Lane 2 : Rat brain lysate
All lanes : Goat Anti-Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution
Predicted band size: 32 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
ab217205 has been referenced in 1 publication.
- Saito K & Ohshima H The putative role of insulin-like growth factor (IGF)-binding protein 5 independent of IGF in the maintenance of pulpal homeostasis in mice. Regen Ther 11:217-224 (2019). PubMed: 31516919