Anti-IGFBP3 antibody (ab77635)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-IGFBP3 antibody
See all IGFBP3 primary antibodies -
DescriptionGoat polyclonal to IGFBP3
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Host speciesGoat
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Specificityab77635 is expected to recognize both reported isoforms (NP_001013416.1 and NP_000589.2).
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Tested applicationsSuitable for: WB, ELISAmore details
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Species reactivityReacts with: Sheep, Human, Pig
Predicted to work with: Mouse, Rat, Horse, Cow, Dog
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Immunogen
Synthetic peptide:
C-RYKVDYESQSTDTQN
, from the internal region of human IGFBP3 (NP_001013416.1; NP_000589.2). -
Positive control
- Recombinant human IGFBP3 protein (ab88368) can be used as a positive control in WB. Human breast cancer lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab77635 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab77635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| ELISA |
Application notes
Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/64000.
WB: Use at a concentration of 0.02 - 0.06 µg/ml. Predicted molecular weight: 32 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.02 - 0.06 µg/ml. Predicted molecular weight: 32 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionIGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
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Tissue specificityExpressed by most tissues. Present in plasma.
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Sequence similaritiesContains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
Developmental stageIGFBP3 levels are higher during extrauterine life and peak during puberty.
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DomainThe thyroglobulin type-1 domain mediates interaction with HN.
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Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 3486 Human
- Entrez Gene: 16009 Mouse
- Entrez Gene: 24484 Rat
- Omim: 146732 Human
- SwissProt: P20959 Cow
- SwissProt: P17936 Human
- SwissProt: P47878 Mouse
- SwissProt: P15473 Rat
see all -
Alternative names
- Acid stable subunit of the 140 K IGF complex antibody
- Binding protein 29 antibody
- Binding protein 53 antibody
see all
Images
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Western blot - Anti-IGFBP3 antibody (ab77635)All lanes : Anti-IGFBP3 antibody (ab77635) at 0.02 µg/ml
Lane 1 : human breast cancer lysate (in RIPA buffer)
Lane 2 : human breast cancer lysate (in RIPA buffer) with immunizing peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 32 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa (possible non-specific binding)
The additional band of 50 kDa was consistently observed. However this band was not blocked by the immunizing peptide and it is therefore a non specific signal.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Yao TH et al. Propranolol induces hemangioma endothelial cell apoptosis via a p53-BAX mediated pathway. Mol Med Rep 18:684-694 (2018). Read more (PubMed: 29767244) »
- Marthandan S et al. Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence. Biol Res 49:34 (2016). Read more (PubMed: 27464526) »
