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Cell Biology Apoptosis Receptors Associated Proteins
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Anti-IGFBP3 antibody (ab77635)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (6)

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Western blot - Anti-IGFBP3 antibody (ab77635)

    Key features and details

    • Goat polyclonal to IGFBP3
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-IGFBP3 antibody
      See all IGFBP3 primary antibodies
    • Description

      Goat polyclonal to IGFBP3
    • Host species

      Goat
    • Specificity

      ab77635 is expected to recognize both reported isoforms (NP_001013416.1 and NP_000589.2).
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Horse, Cow, Dog
    • Immunogen

      Synthetic peptide:

      C-RYKVDYESQSTDTQN

      , from the internal region of human IGFBP3 (NP_001013416.1; NP_000589.2).
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 0.5% BSA, 0.5% Tris buffered saline
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab77635 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Apoptosis
      • Receptors
      • Associated Proteins
      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Phosphatases
      • Cardiovascular
      • Angiogenesis
      • Angiogenic Factors
      • Signal Transduction
      • Growth Factors/Hormones
      • Insulin / Insulin-like
      • Cardiovascular
      • Atherosclerosis
      • Diabetes associated
      • Cardiovascular
      • Atherosclerosis
      • Apoptosis
      • Metabolism
      • Types of disease
      • Obesity
      • Cancer
      • Cell Death
      • Apoptosis
      • Receptors
      • Associated Proteins

    Associated products

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Positive Controls

      • Recombinant human IGFBP3 protein (ab49831)
    • Recombinant Protein

      • Recombinant Human IGFBP3 protein (ab155611)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab77635 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    Notes
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/64000.
    WB: Use at a concentration of 0.02 - 0.06 µg/ml. Predicted molecular weight: 32 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
    • Tissue specificity

      Expressed by most tissues. Present in plasma.
    • Sequence similarities

      Contains 1 IGFBP N-terminal domain.
      Contains 1 thyroglobulin type-1 domain.
    • Developmental stage

      IGFBP3 levels are higher during extrauterine life and peak during puberty.
    • Domain

      The thyroglobulin type-1 domain mediates interaction with HN.
    • Post-translational
      modifications

      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P17936 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3486 Human
      • Entrez Gene: 16009 Mouse
      • Entrez Gene: 24484 Rat
      • Omim: 146732 Human
      • SwissProt: P20959 Cow
      • SwissProt: P17936 Human
      • SwissProt: P47878 Mouse
      • SwissProt: P15473 Rat
      • Unigene: 450230 Human
      • Unigene: 697535 Human
      • Unigene: 29254 Mouse
      • Unigene: 26369 Rat
      see all
    • Alternative names

      • Acid stable subunit of the 140 K IGF complex antibody
      • Binding protein 29 antibody
      • Binding protein 53 antibody
      • BP 53 antibody
      • BP53 antibody
      • Growth hormone dependent binding protein antibody
      • IBP 3 antibody
      • IBP-3 antibody
      • IBP3 antibody
      • IBP3_HUMAN antibody
      • IGF binding protein 3 antibody
      • IGF-binding protein 3 antibody
      • IGFBP 3 antibody
      • IGFBP-3 antibody
      • IGFBP3 antibody
      • Insulin Like Growth Factor Binding Protein 3 antibody
      • Insulin-like growth factor binding protein 3 precursor antibody
      • Insulin-like growth factor-binding protein 3 antibody
      see all

    Images

    • Western blot - Anti-IGFBP3 antibody (ab77635)
      Western blot - Anti-IGFBP3 antibody (ab77635)
      All lanes : Anti-IGFBP3 antibody (ab77635) at 0.02 µg/ml

      Lane 1 : human breast cancer lysate (in RIPA buffer)
      Lane 2 : human breast cancer lysate (in RIPA buffer) with immunizing peptide

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 32 kDa
      Observed band size: 28 kDa why is the actual band size different from the predicted?
      Additional bands at: 50 kDa (possible non-specific binding)



      The additional band of 50 kDa was consistently observed. However this band was not blocked by the immunizing peptide and it is therefore a non specific signal.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (6)

    Publishing research using ab77635? Please let us know so that we can cite the reference in this datasheet.

    ab77635 has been referenced in 6 publications.

    • Nery FC  et al. Impaired kidney structure and function in spinal muscular atrophy. Neurol Genet 5:e353 (2019). PubMed: 31517062
    • Bai R  et al. The NF-?B-modulated miR-19a-3p enhances malignancy of human ovarian cancer cells through inhibition of IGFBP-3 expression. Mol Carcinog 58:2254-2265 (2019). PubMed: 31513316
    • Yao TH  et al. Propranolol induces hemangioma endothelial cell apoptosis via a p53-BAX mediated pathway. Mol Med Rep 18:684-694 (2018). PubMed: 29767244
    • Marthandan S  et al. Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence. Biol Res 49:34 (2016). PubMed: 27464526
    • Goyal R  et al. Antenatal maternal long-term hypoxia: acclimatization responses with altered gene expression in ovine fetal carotid arteries. PLoS One 8:e82200 (2013). WB ; Sheep . PubMed: 24367503
    • Yoo EG  et al. Insulin-like growth factor-binding protein-3 mediates high glucose-induced apoptosis by increasing oxidative stress in proximal tubular epithelial cells. Endocrinology 152:3135-42 (2011). WB ; Pig . PubMed: 21652730

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-IGFBP3 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Sheep Tissue lysate - whole (Liver)
    Loading amount
    3 µg
    Specification
    Liver
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 21 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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