Anti-IGFBP3 antibody (ab77635)

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Overview

  • Product name

    Anti-IGFBP3 antibody
    See all IGFBP3 primary antibodies

  • Description

    Goat polyclonal to IGFBP3

  • Host species

    Goat

  • Specificity

    ab77635 is expected to recognize both reported isoforms (NP_001013416.1 and NP_000589.2).

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Sheep, Human, Pig
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog

  • Immunogen

    Synthetic peptide:

    C-RYKVDYESQSTDTQN

    , from the internal region of human IGFBP3 (NP_001013416.1; NP_000589.2).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Recombinant human IGFBP3 protein (ab88368) can be used as a positive control in WB. Human breast cancer lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/64000.
    WB: Use at a concentration of 0.02 - 0.06 µg/ml. Predicted molecular weight: 32 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

    • Tissue specificity

      Expressed by most tissues. Present in plasma.

    • Sequence similarities

      Contains 1 IGFBP N-terminal domain.
      Contains 1 thyroglobulin type-1 domain.

    • Developmental stage

      IGFBP3 levels are higher during extrauterine life and peak during puberty.

    • Domain

      The thyroglobulin type-1 domain mediates interaction with HN.

    • Post-translational
      modifications

      Phosphorylation sites are present in the extracelllular medium.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Acid stable subunit of the 140 K IGF complex antibody
      • Binding protein 29 antibody
      • Binding protein 53 antibody
      • BP 53 antibody
      • BP53 antibody
      • Growth hormone dependent binding protein antibody
      • IBP 3 antibody
      • IBP-3 antibody
      • IBP3 antibody
      • IBP3_HUMAN antibody
      • IGF binding protein 3 antibody
      • IGF-binding protein 3 antibody
      • IGFBP 3 antibody
      • IGFBP-3 antibody
      • IGFBP3 antibody
      • Insulin Like Growth Factor Binding Protein 3 antibody
      • Insulin-like growth factor binding protein 3 precursor antibody
      • Insulin-like growth factor-binding protein 3 antibody
      see all

    Images

    • Western blot - Anti-IGFBP3 antibody (ab77635)
      Western blot - Anti-IGFBP3 antibody (ab77635)
      All lanes : Anti-IGFBP3 antibody (ab77635) at 0.02 µg/ml

      Lane 1 : human breast cancer lysate (in RIPA buffer)
      Lane 2 : human breast cancer lysate (in RIPA buffer) with immunizing peptide

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 32 kDa
      Observed band size: 28 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 50 kDa (possible non-specific binding)



      The additional band of 50 kDa was consistently observed. However this band was not blocked by the immunizing peptide and it is therefore a non specific signal.

    References

    This product has been referenced in:

    • Yao TH  et al. Propranolol induces hemangioma endothelial cell apoptosis via a p53-BAX mediated pathway. Mol Med Rep 18:684-694 (2018). Read more (PubMed: 29767244) »
    • Marthandan S  et al. Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence. Biol Res 49:34 (2016). Read more (PubMed: 27464526) »
    See all 4 Publications for this product

    Publishing research using ab77635? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Western blot abreview for Anti-IGFBP3 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Sheep Tissue lysate - whole (Liver)
    Loading amount
    3 µg
    Specification
    Liver
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 21 2011

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