Recombinant Anti-IGFBP3 antibody [EPR18680-153] (ab193910)
![Western blot - Anti-IGFBP3 antibody [EPR18680-153] (ab193910)](https://www.abcam.com/ps/products/193/ab193910/Images/ab193910-285403-anti-igfbp3-antibody-epr18680-153-western-blot.jpg "Western blot - Anti-IGFBP3 antibody [EPR18680-153] (ab193910)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18680-153] to IGFBP3
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-IGFBP3 antibody [EPR18680-153]
See all IGFBP3 primary antibodies -
Description
Rabbit monoclonal [EPR18680-153] to IGFBP3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human IGFBP3 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P17936 -
Positive control
- WB: Human serum. IP: Human serum.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18680-153 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab193910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). | |
| IP | 1/70. |
Target
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Function
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
Tissue specificity
Expressed by most tissues. Present in plasma. -
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
Developmental stage
IGFBP3 levels are higher during extrauterine life and peak during puberty. -
Domain
The thyroglobulin type-1 domain mediates interaction with HN. -
Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3486 Human
- Omim: 146732 Human
- SwissProt: P17936 Human
- Unigene: 450230 Human
- Unigene: 697535 Human
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Alternative names
- Acid stable subunit of the 140 K IGF complex antibody
- Binding protein 29 antibody
- Binding protein 53 antibody
see all
Images
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Western blot - Anti-IGFBP3 antibody [EPR18680-153] (ab193910)Anti-IGFBP3 antibody [EPR18680-153] (ab193910) at 1/1000 dilution + Human serum at 10 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution (VeriBlot for IP Detection Reagent (HRP))
Developed using the ECL technique.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Exposure time: 10 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-IGFBP3 antibody [EPR18680-153] (ab193910)](https://www.abcam.com/ps/products/193/ab193910/Images/ab193910-285404-anti-igfbp3-antibody-epr18680-153-immunoprecipitation.jpg)
Immunoprecipitation - Anti-IGFBP3 antibody [EPR18680-153] (ab193910)IGFBP3 was immunoprecipitated from 0.35 mg of human serum with ab193910 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab193910 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Human serum 10 μg (Input).
Lane 2: ab193910 IP in human serum.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab193910 in human serum.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
The bands at 41 kDa and 44 kDa have previously been documented as IGFBP3 doublets (PMID:16816363).
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![Anti-IGFBP3 antibody [EPR18680-153] (ab193910)](https://www.abcam.com/ps/products/193/ab193910/Images/ab193910-3-benefits-of-recombinant-antibodies.png)
Anti-IGFBP3 antibody [EPR18680-153] (ab193910)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab193910 has not yet been referenced specifically in any publications.
