Recombinant
RabMAb

Recombinant Anti-IGFBP3 antibody [EPR18680-153] (ab193910)

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Overview

  • Product name

    Anti-IGFBP3 antibody [EPR18680-153]
    See all IGFBP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR18680-153] to IGFBP3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human IGFBP3 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P17936

  • Positive control

    • WB: Human serum. IP: Human serum.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab193910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
IP 1/70.

Target

  • Function

    IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

  • Tissue specificity

    Expressed by most tissues. Present in plasma.

  • Sequence similarities

    Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.

  • Developmental stage

    IGFBP3 levels are higher during extrauterine life and peak during puberty.

  • Domain

    The thyroglobulin type-1 domain mediates interaction with HN.

  • Post-translational
    modifications

    Phosphorylation sites are present in the extracelllular medium.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • Acid stable subunit of the 140 K IGF complex antibody
      • Binding protein 29 antibody
      • Binding protein 53 antibody
      • BP 53 antibody
      • BP53 antibody
      • Growth hormone dependent binding protein antibody
      • IBP 3 antibody
      • IBP-3 antibody
      • IBP3 antibody
      • IBP3_HUMAN antibody
      • IGF binding protein 3 antibody
      • IGF-binding protein 3 antibody
      • IGFBP 3 antibody
      • IGFBP-3 antibody
      • IGFBP3 antibody
      • Insulin Like Growth Factor Binding Protein 3 antibody
      • Insulin-like growth factor binding protein 3 precursor antibody
      • Insulin-like growth factor-binding protein 3 antibody
      see all

    Images

    • Western blot - Anti-IGFBP3 antibody [EPR18680-153] (ab193910)
      Western blot - Anti-IGFBP3 antibody [EPR18680-153] (ab193910)
      Anti-IGFBP3 antibody [EPR18680-153] (ab193910) at 1/1000 dilution + Human serum at 10 µg

      Secondary
      VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution (VeriBlot for IP Detection Reagent (HRP))

      Developed using the ECL technique.

      Predicted band size: 32 kDa
      Observed band size: 32 kDa


      Exposure time: 10 seconds


      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-IGFBP3 antibody [EPR18680-153] (ab193910)
      Immunoprecipitation - Anti-IGFBP3 antibody [EPR18680-153] (ab193910)

      IGFBP3 was immunoprecipitated from 0.35 mg of human serum with ab193910 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab193910 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: Human serum 10 μg (Input).

      Lane 2: ab193910 IP in human serum.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab193910 in human serum.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 seconds.

      The bands at 41 kDa and 44 kDa have previously been documented as IGFBP3 doublets (PMID:16816363).

    References

    ab193910 has not yet been referenced specifically in any publications.

    Publishing research using ab193910? Please let us know so that we can cite the reference in this datasheet.

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