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    igfbp3-antibody-epr18680-153-ab193910.pdf

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Cell Biology Apoptosis Receptors Associated Proteins
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RecombinantRabMAb

Recombinant Anti-IGFBP3 antibody [EPR18680-153] (ab193910)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-IGFBP3 antibody [EPR18680-153] (ab193910)
  • Immunoprecipitation - Anti-IGFBP3 antibody [EPR18680-153] (ab193910)
  • Anti-IGFBP3 antibody [EPR18680-153] (ab193910)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18680-153] to IGFBP3
  • Suitable for: WB, IP
  • Reacts with: Human

You may also be interested in

Protein
Product image
Recombinant Human IGFBP3 protein (ab155611)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-IGFBP3 antibody [EPR18680-153]
    See all IGFBP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR18680-153] to IGFBP3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human IGFBP3 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P17936

  • Positive control

    • WB: Human serum. IP: Human serum.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18680-153
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Receptors
    • Associated Proteins
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Phosphatases
    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors
    • Signal Transduction
    • Growth Factors/Hormones
    • Insulin / Insulin-like
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cardiovascular
    • Atherosclerosis
    • Apoptosis
    • Metabolism
    • Types of disease
    • Obesity
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Associated Proteins

Associated products

  • Alternative Versions

    • Anti-IGFBP3 antibody [EPR18680-153] - BSA and Azide free (ab224530)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human IGFBP3 protein (ab155611)

Applications

Our Abpromise guarantee covers the use of ab193910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
IP 1/70.

Target

  • Function

    IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • Tissue specificity

    Expressed by most tissues. Present in plasma.
  • Sequence similarities

    Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • Developmental stage

    IGFBP3 levels are higher during extrauterine life and peak during puberty.
  • Domain

    The thyroglobulin type-1 domain mediates interaction with HN.
  • Post-translational
    modifications

    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P17936 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3486 Human
    • Omim: 146732 Human
    • SwissProt: P17936 Human
    • Unigene: 450230 Human
    • Unigene: 697535 Human
    • Alternative names

      • Acid stable subunit of the 140 K IGF complex antibody
      • Binding protein 29 antibody
      • Binding protein 53 antibody
      • BP 53 antibody
      • BP53 antibody
      • Growth hormone dependent binding protein antibody
      • IBP 3 antibody
      • IBP-3 antibody
      • IBP3 antibody
      • IBP3_HUMAN antibody
      • IGF binding protein 3 antibody
      • IGF-binding protein 3 antibody
      • IGFBP 3 antibody
      • IGFBP-3 antibody
      • IGFBP3 antibody
      • Insulin Like Growth Factor Binding Protein 3 antibody
      • Insulin-like growth factor binding protein 3 precursor antibody
      • Insulin-like growth factor-binding protein 3 antibody
      see all

    Images

    • Western blot - Anti-IGFBP3 antibody [EPR18680-153] (ab193910)
      Western blot - Anti-IGFBP3 antibody [EPR18680-153] (ab193910)
      Anti-IGFBP3 antibody [EPR18680-153] (ab193910) at 1/1000 dilution + Human serum at 10 µg

      Secondary
      VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/2000 dilution (VeriBlot for IP Detection Reagent (HRP))

      Developed using the ECL technique.

      Predicted band size: 32 kDa
      Observed band size: 32 kDa


      Exposure time: 10 seconds


      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-IGFBP3 antibody [EPR18680-153] (ab193910)
      Immunoprecipitation - Anti-IGFBP3 antibody [EPR18680-153] (ab193910)

      IGFBP3 was immunoprecipitated from 0.35 mg of human serum with ab193910 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab193910 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: Human serum 10 μg (Input).

      Lane 2: ab193910 IP in human serum.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab193910 in human serum.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 seconds.

      The bands at 41 kDa and 44 kDa have previously been documented as IGFBP3 doublets (PMID:16816363).

    • Anti-IGFBP3 antibody [EPR18680-153] (ab193910)
      Anti-IGFBP3 antibody [EPR18680-153] (ab193910)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab193910? Please let us know so that we can cite the reference in this datasheet.

    ab193910 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab193910.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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