Overview

  • Product name

  • Description

    Goat polyclonal to IGFBP4
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Goat, Chicken, Cow, Pig
  • Immunogen

    Synthetic peptide:

    KGELDCHQLADSFRE

    , corresponding to C terminal amino acids 244-258 of Human IGFBP4 (NP_001543.2).

  • Positive control

    • Recombinant Human IGFBP4 protein (ab69504) can be used as a positive control in WB. Human Liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/8000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    References

    This product has been referenced in:

    • Contois LW  et al. Insulin-like growth factor binding protein-4 differentially inhibits growth factor-induced angiogenesis. J Biol Chem 287:1779-89 (2012). WB ; Human . Read more (PubMed: 22134921) »
    • Gardner S  et al. TGF-ß inhibits muscle differentiation by blocking autocrine signaling pathways initiated by IGF-II. Mol Endocrinol 25:128-37 (2011). WB ; Mouse . Read more (PubMed: 21106882) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (murine breast cancer cell line monolayer)
    Permeabilization
    Yes - 0,01 % TritonX-100
    Specification
    murine breast cancer cell line monolayer
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Apr 08 2019

    Question
    Answer


    I am very pleased to hear you would like to accept our offer and test ab77350 in IHC-P. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More
    Application
    Western blot
    Sample
    Mouse Cell lysate - other (serum- 3 microliter)
    Specification
    serum- 3 microliter
    Gel Running Conditions
    Reduced Denaturing (8)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

    Ru Contois

    Verified customer

    Submitted Nov 11 2009

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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