Anti-IGFBP4 antibody (ab77350)
Key features and details
- Goat polyclonal to IGFBP4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-IGFBP4 antibody
See all IGFBP4 primary antibodies -
Description
Goat polyclonal to IGFBP4 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Sheep, Goat, Chicken, Cow, Pig -
Immunogen
Synthetic peptide:
KGELDCHQLADSFRE
, corresponding to C terminal amino acids 244-258 of Human IGFBP4 (NP_001543.2). -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Notes |
---|
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
-
Function
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 374271 Chicken
- Entrez Gene: 282262 Cow
- Entrez Gene: 3487 Human
- Entrez Gene: 360622 Rat
- Entrez Gene: 443470 Sheep
- Omim: 146733 Human
- SwissProt: Q05716 Cow
- SwissProt: P22692 Human
see all -
Alternative names
- AI875747 antibody
- BP 4 antibody
- BP4 antibody
see all
Images
-
Anti-IGFBP4 antibody (ab77350) at 1 µg/ml + Human Liver lysate in RIPA buffer at 35 µg
Predicted band size: 28 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Datasheets and documents
-
Datasheet download
References (3)
ab77350 has been referenced in 3 publications.
- Madani AY et al. Signal Transducer and Activator of Transcription 3 (STAT3) Suppresses STAT1/Interferon Signaling Pathway and Inflammation in Senescent Preadipocytes. Antioxidants (Basel) 10:N/A (2021). PubMed: 33672392
- Contois LW et al. Insulin-like growth factor binding protein-4 differentially inhibits growth factor-induced angiogenesis. J Biol Chem 287:1779-89 (2012). WB ; Human . PubMed: 22134921
- Gardner S et al. TGF-ß inhibits muscle differentiation by blocking autocrine signaling pathways initiated by IGF-II. Mol Endocrinol 25:128-37 (2011). WB ; Mouse . PubMed: 21106882