Overview

  • Product name
  • Description
    Rabbit polyclonal to IGFBP4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (RALERLAASQ SRTHEDLYII PIPNCDRNGN FHPKQCHPAL DGQRGKCWCV) of human IGFBP4 (NP_001543).

  • Positive control
    • Fetal brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23660500
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.

Target

Images

  • Anti-IGFBP4 antibody (ab83846) at 1 µg/ml + fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa

  • ab83846 at 5 µg/ml staining IGFBP1 in Human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab83846 at 5 µg/ml staining IGFBP1 in Human placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • ab83846 at 5 µg/ml staining IGFBP1 in Human prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of mouse small intestine tissue, staining IGFBP4 with ab83846.

    Tissue was fixed with formaldehyde and blocked with 5% BSA for 1 hour at 25°C; antigen retrieval was by heat mediation in Tris/EDTA buffer (pH 8). Samples were incubated with primary antibody (1/200 in PBS-T) for 12 hours at 4°C. An AlexaFluor®555-conjugated donkey anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Namvar S  et al. Functional molecules in mesothelial-to-mesenchymal transition revealed by transcriptome analyses. J Pathol 245:491-501 (2018). Read more (PubMed: 29774544) »
  • Thomsen J  et al. PAPP-A proteolytic activity enhances IGF bioactivity in ascites from women with ovarian carcinoma. Oncotarget 6:32266-78 (2015). IHC . Read more (PubMed: 26336825) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (heart)
Permeabilization
Yes - 0.3% Triton X
Specification
heart
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 20% · Temperature: 25°C
Fixative
Paraformaldehyde

Suwannee Thet

Verified customer

Submitted Jun 18 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (small intestine)
Specification
small intestine
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TE (PH8.0)
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 28 2012

Answer

Thank you for your Abreview.

I have returned the Abreview to you so that you may enter the discount code. Please include this code in the additional notes section and re-submit the Abreview. After submission of the Abreview the code will activate and will be able to be redeemed for a free primary antibody.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

I am very pleased to hear you would like to accept our offer and test ab83846 inmouse. This code will give you 1 free antibody before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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