Anti-IGFBP4 antibody [C15] (ab239594)
Key features and details
- Mouse monoclonal [C15] to IGFBP4
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
-
Product name
Anti-IGFBP4 antibody [C15]
See all IGFBP4 primary antibodies -
Description
Mouse monoclonal [C15] to IGFBP4 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Sheep, Cow, Pig -
Immunogen
Recombinant full length protein (His-tag) corresponding to Human IGFBP4 aa 1 to the C-terminus. N- terminal tag (Expressed in E.coli).
Database link: P22692 -
Positive control
- IHC-P: Human liver and colon tissues. WB: Rat placenta and uterus lysates; Recombinant human IGFBP4 protein.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
-
Purity
Protein A/G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
C15 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab239594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 28 kDa.
|
|
IHC-P |
Use a concentration of 5 - 30 µg/ml.
|
Notes |
---|
WB
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 28 kDa. |
IHC-P
Use a concentration of 5 - 30 µg/ml. |
Target
-
Function
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 282262 Cow
- Entrez Gene: 3487 Human
- Entrez Gene: 360622 Rat
- Entrez Gene: 443470 Sheep
- Omim: 146733 Human
- SwissProt: Q05716 Cow
- SwissProt: P22692 Human
- SwissProt: P24854 Pig
see all -
Alternative names
- AI875747 antibody
- BP 4 antibody
- BP4 antibody
see all
Images
-
Paraffin-embedded human colon tissue stained for IGFBP4 using ab239594 at 10 µg/ml in immunohistochemical analysis. DAB staining.
-
Anti-IGFBP4 antibody [C15] (ab239594) at 2 µg/ml + Rat placenta lysate
Secondary
HRP-Goat Anti-Mouse IgG at 0.2 µg/ml
Predicted band size: 28 kDa -
Paraffin-embedded human liver tissue stained for IGFBP4 using ab239594 at 30 µg/ml in immunohistochemical analysis. DAB staining.
-
Anti-IGFBP4 antibody [C15] (ab239594) at 2 µg/ml + Rat uterus lysate
Secondary
HRP-Goat Anti-Mouse IgG at 0.2 µg/ml
Predicted band size: 28 kDa -
Anti-IGFBP4 antibody [C15] (ab239594) at 5 µg/ml + Recombinant human IGFBP4 protein
Predicted band size: 28 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab239594 has not yet been referenced specifically in any publications.