Overview

  • Product name

    Anti-IGFBP4 antibody [EPR19762]
    See all IGFBP4 primary antibodies
  • Description

    Rabbit monoclonal [EPR19762] to IGFBP4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein within Human IGFBP4 aa 1 to the C-terminus. The exact sequence is proprietary. MA added at N-Terminal.
    Database link: P22692

  • Positive control

    • WB: A549 and HT-29 whole cell lysates; Human placenta lysate. Flow Cyt: A549 cells. IP: A549 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab205581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 28, 18 kDa (predicted molecular weight: 28 kDa).
Flow Cyt 1/500.
IP 1/30.

Target

  • Function

    IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • Sequence similarities

    Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI875747 antibody
    • BP 4 antibody
    • BP4 antibody
    • Deb2 antibody
    • HT29 IGFBP antibody
    • IBP 4 antibody
    • IBP-4 antibody
    • IBP4 antibody
    • IBP4_HUMAN antibody
    • IGF binding protein 4 antibody
    • IGF-binding protein 4 antibody
    • IGF-BP4 antibody
    • IGFBP 4 antibody
    • IGFBP-4 antibody
    • IGFBP4 antibody
    • Insulin Like Growth Factor Binding Protein 4 antibody
    • Insulin-like growth factor-binding protein 4 antibody
    see all

Images

  • Anti-IGFBP4 antibody [EPR19762] (ab205581) at 1/1000 dilution + A549 (human lung carcinoma cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 28 kDa
    Observed band size: 18,28 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human IGFBP4 has 2 isoforms detected at 28 kDa and 18 kDa.

  • Anti-IGFBP4 antibody [EPR19762] (ab205581) at 1/1000 dilution + human placenta lysate at 10 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-IGFBP4 antibody [EPR19762] (ab205581) at 1/1000 dilution + HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed A549 (human lung carcinoma cell line) cell line labeling IGFBP4 with ab205581 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • IGFBP4 was immunoprecipitated from 0.35 mg of A549 (human lung carcinoma cell line) whole cell lysate with ab205581 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab205581 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: A549 whole cell lysate 10 µg (Input). 

    Lane 2: ab205581 IP in A549 whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205581 in A549 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    Note: Human IGFBP4 has 2 isoforms detected at 28 kDa and 18 kDa.

References

ab205581 has not yet been referenced specifically in any publications.

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