Recombinant Anti-IGFBP6 antibody [EPR21809] (ab219560)
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- References
- Protocols
Overview
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Product name
Anti-IGFBP6 antibody [EPR21809]
See all IGFBP6 primary antibodies -
Description
Rabbit monoclonal [EPR21809] to IGFBP6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human IGFBP6 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P24592 -
Positive control
- WB: Human IGFBP6 recombinant protein; Human placenta and kidney lysates; NCI-H1299 and HaCaT whole cell lysates. ICC/IF: HaCaT cells. Flow Cyt: HaCaT cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21809 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab219560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 25 kDa). | |
| ICC/IF | 1/100. | |
| Flow Cyt | 1/500. |
Target
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Function
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
Post-translational
modificationsO-linked glycans consist of hexose (probably Gal), N-acetylhexosamine (probably GalNAc) and sialic acid residues. O-glycosylated with core 1 or possibly core 8 glycans. O-glycosylated on one site only in the region AA 143-168 in cerebrospinal fluid. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3489 Human
- SwissProt: P24592 Human
- Unigene: 274313 Human
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Alternative names
- IBP 6 antibody
- IBP-6 antibody
- IBP6 antibody
see all
Images
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![Western blot - Anti-IGFBP6 antibody [EPR21809] (ab219560)](https://www.abcam.com/ps/products/219/ab219560/Images/ab219560-306880-anti-igfbp6-antibody-epr21809-western-blot.jpg)
Western blot - Anti-IGFBP6 antibody [EPR21809] (ab219560)All lanes : Anti-IGFBP6 antibody [EPR21809] (ab219560) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lane 2 : Human kidney lysate
Lane 3 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lane 4 : HaCaT (human keratinocyte cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time: 59 secondsBlocking/dilution buffer and concentration: 5% NFDM/TBST.
The molecular mass observed is consistent with the literature (PMID 27623076; PMID 21820463).
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![Immunocytochemistry/ Immunofluorescence - Anti-IGFBP6 antibody [EPR21809] (ab219560)](https://www.abcam.com/ps/products/219/ab219560/Images/ab219560-306881-anti-igfbp6-antibody-epr21809-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IGFBP6 antibody [EPR21809] (ab219560)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HaCaT (human keratinocyte cell line) cells labeling IGFBP6 with ab219560 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weakly nuclear staining in HaCaT cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Western blot - Anti-IGFBP6 antibody [EPR21809] (ab219560)](https://www.abcam.com/ps/products/219/ab219560/Images/ab219560-306879-anti-igfbp6-antibody-epr21809-western-blot.jpg)
Western blot - Anti-IGFBP6 antibody [EPR21809] (ab219560)Anti-IGFBP6 antibody [EPR21809] (ab219560) at 1/5000 dilution + Human IGFBP6 recombinant protein (aa25-240) from mammalian expression system at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/diluting buffer and concentration: 5% NFDM/TBST.
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![Flow Cytometry - Anti-IGFBP6 antibody [EPR21809] (ab219560)](https://www.abcam.com/ps/products/219/ab219560/Images/ab219560-306878-anti-igfbp6-antibody-epr21809-flow-cytometry.jpg)
Flow Cytometry - Anti-IGFBP6 antibody [EPR21809] (ab219560)Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HaCaT (human keratinocyte cell line) cell line labeling IGFBP6 with ab219560 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Datasheets and documents
References
ab219560 has not yet been referenced specifically in any publications.
