Recombinant
RabMAb

Recombinant Anti-IGFBP6 antibody [EPR21809] (ab219560)

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Overview

  • Product name

    Anti-IGFBP6 antibody [EPR21809]
    See all IGFBP6 primary antibodies

  • Description

    Rabbit monoclonal [EPR21809] to IGFBP6

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human IGFBP6 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P24592

  • Positive control

    • WB: Human IGFBP6 recombinant protein; Human placenta and kidney lysates; NCI-H1299 and HaCaT whole cell lysates. ICC/IF: HaCaT cells. Flow Cyt: HaCaT cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab219560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 25 kDa).
ICC/IF 1/100.
Flow Cyt 1/500.

Target

  • Function

    IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

  • Sequence similarities

    Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.

  • Post-translational
    modifications

    O-linked glycans consist of hexose (probably Gal), N-acetylhexosamine (probably GalNAc) and sialic acid residues. O-glycosylated with core 1 or possibly core 8 glycans. O-glycosylated on one site only in the region AA 143-168 in cerebrospinal fluid.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • IBP 6 antibody
      • IBP-6 antibody
      • IBP6 antibody
      • IBP6_HUMAN antibody
      • IGF binding protein 6 antibody
      • IGF-binding protein 6 antibody
      • IGFBP 6 antibody
      • IGFBP-6 antibody
      • IGFBP6 antibody
      • Insulin like growth factor binding protein 6 antibody
      • Insulin-like growth factor-binding protein 6 antibody
      see all

    Images

    • Western blot - Anti-IGFBP6 antibody [EPR21809] (ab219560)
      Western blot - Anti-IGFBP6 antibody [EPR21809] (ab219560)
      All lanes : Anti-IGFBP6 antibody [EPR21809] (ab219560) at 1/1000 dilution

      Lane 1 : Human placenta lysate
      Lane 2 : Human kidney lysate
      Lane 3 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
      Lane 4 : HaCaT (human keratinocyte cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 25 kDa
      Observed band size: 34 kDa
      why is the actual band size different from the predicted?


      Exposure time: 59 seconds


      Blocking/dilution buffer and concentration: 5% NFDM/TBST.

      The molecular mass observed is consistent with the literature (PMID 27623076; PMID 21820463).

    • Immunocytochemistry/ Immunofluorescence - Anti-IGFBP6 antibody [EPR21809] (ab219560)
      Immunocytochemistry/ Immunofluorescence - Anti-IGFBP6 antibody [EPR21809] (ab219560)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HaCaT (human keratinocyte cell line) cells labeling IGFBP6 with ab219560 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weakly nuclear staining in HaCaT cell line.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Western blot - Anti-IGFBP6 antibody [EPR21809] (ab219560)
      Western blot - Anti-IGFBP6 antibody [EPR21809] (ab219560)
      Anti-IGFBP6 antibody [EPR21809] (ab219560) at 1/5000 dilution + Human IGFBP6 recombinant protein (aa25-240) from mammalian expression system at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 25 kDa
      Observed band size: 34 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    • Flow Cytometry - Anti-IGFBP6 antibody [EPR21809] (ab219560)
      Flow Cytometry - Anti-IGFBP6 antibody [EPR21809] (ab219560)

      Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HaCaT (human keratinocyte cell line) cell line labeling IGFBP6 with ab219560 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    References

    ab219560 has not yet been referenced specifically in any publications.

    Publishing research using ab219560? Please let us know so that we can cite the reference in this datasheet.

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