RecombinantRabMAb

Recombinant Anti-IGFBP6 antibody [EPR21809] (ab219560)

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Western blot - Anti-IGFBP6 antibody [EPR21809] (ab219560)
  • Immunocytochemistry/ Immunofluorescence - Anti-IGFBP6 antibody [EPR21809] (ab219560)
  • Western blot - Anti-IGFBP6 antibody [EPR21809] (ab219560)
  • Flow Cytometry - Anti-IGFBP6 antibody [EPR21809] (ab219560)
  • Anti-IGFBP6 antibody [EPR21809] (ab219560)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21809] to IGFBP6
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-IGFBP6 antibody [EPR21809]
    See all IGFBP6 primary antibodies

  • Description

    Rabbit monoclonal [EPR21809] to IGFBP6

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human IGFBP6 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P24592

  • Positive control

    • WB: Human IGFBP6 recombinant protein; Human placenta and kidney lysates; NCI-H1299 and HaCaT whole cell lysates. ICC/IF: HaCaT cells. Flow Cyt: HaCaT cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab219560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 25 kDa).
ICC/IF 1/100.
Flow Cyt 1/500.

Target

  • Function

    IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

  • Sequence similarities

    Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.

  • Post-translational
    modifications

    O-linked glycans consist of hexose (probably Gal), N-acetylhexosamine (probably GalNAc) and sialic acid residues. O-glycosylated with core 1 or possibly core 8 glycans. O-glycosylated on one site only in the region AA 143-168 in cerebrospinal fluid.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • IBP 6 antibody
      • IBP-6 antibody
      • IBP6 antibody
      • IBP6_HUMAN antibody
      • IGF binding protein 6 antibody
      • IGF-binding protein 6 antibody
      • IGFBP 6 antibody
      • IGFBP-6 antibody
      • IGFBP6 antibody
      • Insulin like growth factor binding protein 6 antibody
      • Insulin-like growth factor-binding protein 6 antibody
      see all

    Images

    • Western blot - Anti-IGFBP6 antibody [EPR21809] (ab219560)
      Western blot - Anti-IGFBP6 antibody [EPR21809] (ab219560)
      All lanes : Anti-IGFBP6 antibody [EPR21809] (ab219560) at 1/1000 dilution

      Lane 1 : Human placenta lysate
      Lane 2 : Human kidney lysate
      Lane 3 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
      Lane 4 : HaCaT (human keratinocyte cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 25 kDa
      Observed band size: 34 kDa
      why is the actual band size different from the predicted?


      Exposure time: 59 seconds


      Blocking/dilution buffer and concentration: 5% NFDM/TBST.

      The molecular mass observed is consistent with the literature (PMID 27623076; PMID 21820463).

    • Immunocytochemistry/ Immunofluorescence - Anti-IGFBP6 antibody [EPR21809] (ab219560)
      Immunocytochemistry/ Immunofluorescence - Anti-IGFBP6 antibody [EPR21809] (ab219560)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HaCaT (human keratinocyte cell line) cells labeling IGFBP6 with ab219560 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weakly nuclear staining in HaCaT cell line.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Western blot - Anti-IGFBP6 antibody [EPR21809] (ab219560)
      Western blot - Anti-IGFBP6 antibody [EPR21809] (ab219560)
      Anti-IGFBP6 antibody [EPR21809] (ab219560) at 1/5000 dilution + Human IGFBP6 recombinant protein (aa25-240) from mammalian expression system at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 25 kDa
      Observed band size: 34 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    • Flow Cytometry - Anti-IGFBP6 antibody [EPR21809] (ab219560)
      Flow Cytometry - Anti-IGFBP6 antibody [EPR21809] (ab219560)

      Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HaCaT (human keratinocyte cell line) cell line labeling IGFBP6 with ab219560 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    • Anti-IGFBP6 antibody [EPR21809] (ab219560)
      Anti-IGFBP6 antibody [EPR21809] (ab219560)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab219560? Please let us know so that we can cite the reference in this datasheet.

    ab219560 has not yet been referenced specifically in any publications.

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