Overview

  • Product name

  • Description

    Rabbit polyclonal to IGFBP7
  • Host species

    Rabbit
  • Specificity

    This antibody reacts with IGFBP7. It does not recognize IGFBPs 1,2,3,4,5,or 6.
  • Tested applications

    Suitable for: ELISA, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Epitope of human IGFBP7.

  • General notes

    Dissolved in 400 µl sterile water (a 1/20 dilution). Dilute further in a buffer solution appropriate for the desired application.

Properties

Applications

Our Abpromise guarantee covers the use of ab36661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/500.
IHC-Fr 1/500.

Target

  • Function

    Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 Kazal-like domain.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • AGM antibody
    • Angiomodulin antibody
    • FSTL2 antibody
    • IBP-7 antibody
    • IBP7 antibody
    • IBP7_HUMAN antibody
    • IGF binding protein 7 antibody
    • IGF-binding protein 7 antibody
    • IGFBP rP1 antibody
    • IGFBP-7 antibody
    • IGFBP-rP1 antibody
    • IGFBP7 antibody
    • IGFBPRP1 antibody
    • Insulin like growth factor binding protein 7 antibody
    • Insulin-like growth factor-binding protein 7 antibody
    • MAC25 antibody
    • MAC25 protein antibody
    • PGI2 stimulating factor antibody
    • PGI2-stimulating factor antibody
    • Prostacyclin stimulating factor antibody
    • Prostacyclin-stimulating factor antibody
    • PSF antibody
    • RAMSVPS antibody
    • TAF antibody
    • Tumor-derived adhesion factor antibody
    see all

Images

  • ab36661 (4µg/ml) staining IGFBP7 in human renal cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining of renal tubules.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References

ab36661 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

I have processed your request to receive ab74169 as a replacement. Your new order number is *****. As this is shipping to Canada, I have requested the antibody be shipped on Monday, as if it ships today, it would arrive on Saturday and I was not sure if there would be someone there to receive it. If there is anything else I can help you with, please let me know.

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Answer

Having reviewed all of the data that you kindly sent, it is clear that ab36661 is not working as stated on the datasheet. As it is covered under our Abpromise I would be happy to send you either a replacement antibody or to process a refund. We do not currently have a different lot of ab36661 and so I would not advice sending another vial of that antibody, but I believe ab74169 would be a suitable alternative.

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Answer

Thank you for sending the information. The only other information that would be helpful is if you could confirm the species that the tissues came from, was it human? Also do you have information on the "sensitive polymer kit" and the antigen retrieval methods used? I do appreciate your patience in this matter; we just like to get as much information as possible as to why our antibodies are working and how they are being tested. I look forward to your reply.

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Answer

Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody. I look forward to receiving your reply. PS : Please fill the questionaire in relation to the application used.         There were 5 vials of this product in your order? Could you specify if all of these vials are faulty?  

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