Key features and details
- Rabbit polyclonal to IGFBP7
- Suitable for: IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-IGFBP7 antibody
See all IGFBP7 primary antibodies
DescriptionRabbit polyclonal to IGFBP7
SpecificityThis antibody reacts with IGFBP7. It does not recognize IGFBPs 1,2,3,4,5,or 6.
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Rat, Human
Epitope of human IGFBP7.
General notesDissolved in 400 µl sterile water (a 1/20 dilution). Dilute further in a buffer solution appropriate for the desired application.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Concentration information loading...
PurityProtein A purified
Purification notesThis antibody is affinity purified.
Our Abpromise guarantee covers the use of ab36661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionBinds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion.
Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 Kazal-like domain.
- Information by UniProt
- AGM antibody
- Angiomodulin antibody
- FSTL2 antibody
ab36661 (4µg/ml) staining IGFBP7 in human renal cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining of renal tubules.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ab36661 has not yet been referenced specifically in any publications.