Overview

  • Product name

  • Description

    Rabbit polyclonal to IGFBP7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Rainbow trout
  • Immunogen

    Synthetic peptide corresponding to Human IGFBP7 aa 200 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab85821)

  • Positive control

    • Recombinant Human IGFBP7 protein (ab50195) can be used as a positive control in WB. IHC-P: FFPE human pancreas.

Properties

Applications

Our Abpromise guarantee covers the use of ab74169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 - 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 29 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration. PubMed: 21873981

Target

  • Function

    Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 Kazal-like domain.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • AGM antibody
    • Angiomodulin antibody
    • FSTL2 antibody
    • IBP-7 antibody
    • IBP7 antibody
    • IBP7_HUMAN antibody
    • IGF binding protein 7 antibody
    • IGF-binding protein 7 antibody
    • IGFBP rP1 antibody
    • IGFBP-7 antibody
    • IGFBP-rP1 antibody
    • IGFBP7 antibody
    • IGFBPRP1 antibody
    • Insulin like growth factor binding protein 7 antibody
    • Insulin-like growth factor-binding protein 7 antibody
    • MAC25 antibody
    • MAC25 protein antibody
    • PGI2 stimulating factor antibody
    • PGI2-stimulating factor antibody
    • Prostacyclin stimulating factor antibody
    • Prostacyclin-stimulating factor antibody
    • PSF antibody
    • RAMSVPS antibody
    • TAF antibody
    • Tumor-derived adhesion factor antibody
    see all

Images

  • IHC image of IGFBP7 staining in a formalin fixed, paraffin embedded normal human pancreas tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74169 at 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Anti-IGFBP7 antibody (ab74169) at 1 µg/ml + Recombinant Human IGFBP7 protein (ab50195) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa
    Observed band size: 29 kDa


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab74169 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • ICC/IF image of ab74169 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74169, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 1µg/ml.

References

This product has been referenced in:

  • Ren JJ  et al. Insulin-like growth factor binding protein related protein 1 knockdown attenuates hepatic fibrosis via the regulation of MMPs/TIMPs in mice. Hepatobiliary Pancreat Dis Int 18:38-47 (2019). Read more (PubMed: 30243878) »
  • Cai X  et al. Silence of IGFBP7 suppresses apoptosis and epithelial mesenchymal transformation of high glucose induced-podocytes. Exp Ther Med 16:1095-1102 (2018). Read more (PubMed: 30112052) »
See all 9 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Non-Denaturing (Native)
Sample
Human Serum (conditioned medium)
Specification
conditioned medium
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 08 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15% gel)
Sample
Human Purified protein (periodontal ligament cell-conditioned medium)
Specification
periodontal ligament cell-conditioned medium
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Oct 29 2014

Answer

Thank you for contacting us.
While the immunogen sequences for our antibodies are considered proprietary, we do provide general information about the immunogen range on our datasheets:
ab38972: Synthetic peptide based on the Human sequence. The antibody epitope is located in loop 3 of TIMP2. The antibody does not cross-react with TIMP1, TIMP3, or TIMP4.
ab38973: Synthetic peptide based on Human TIMP2 sequence. The epitope is located in loop #1 of TIMP2. The antibody does not cross-react with TIMP1, TIMP3, or TIMP4.
ab53730: Synthetic peptide derived from human TIMP2.
ab28260: This antibody is made against a synthetic peptide based on loop 2 of human TIMP2, which may be more bioavailable than the amino terminal or carboxy terminal locations. The antibody does not cross-react with TIMP1, TIMP3, or TIMP4.
ab74169: Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human IGFBP7
We are also unable to disclose the sequence for peptide ab41149 because it was used as the immunogen for ab38973.
I hope this helps, please let me know if you need any additional information or assistance.

Read More

Answer

Thank you for your response.

This is to confirm thatI have placed a new order for you - for one vial ofab74169 as a free of charge replacement and the new order number is1065537 and the item will be shipped next Tuesday (10th April) after Easter break.

I hope the second vial will work as it is expected, and please do let me know how you are getting on with this product.

Read More

Answer

Thank you for your response. The discount code has been issued for you for testing this antibody in IP. I am forwarding my colleague's e-mail below so please read the discount offer. If you have any questions, please do not hesitate to contact us again.



Nevertheless, I can certainly offer you a new vial (feel of charge replacement) for you if this antibody does no longer work in Western blot and IHC. Let me know if you wish to get a new vial.

Previous e-mail:

Thank you for contacting us.

Your order has the reference number 1024589 and you should receive it as discussed by 06 February.

I am very pleased to hear you would like to accept our offer and test ab74169 in IP.

DISCOUNT CODE:100%ABR-TXXXXX
Expiration date: 01 June 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount

Read More

Answer

Thank you for your contact regarding ab74169. I am very sorry to hear that you are having problem with this antibody.

I understand from your e-mail this antibody has been used in Western blot and IHC application and it worked initially but failed to work later on. According to our record, testing discount code was offered to you to test one vial ab74169 in immunoprecipitation (IP).

Have you had a chance to use this product in IP?
Would you be so kind to explain how the antibody was stored after arrival?



I look forward to hearing from you and hope to solve this problem as soon as possible.

Read More

Answer

Thank you for contacting us. The western blot data on our website was generated under reduced and denatured conditions. The antibody may also be suitable for native WB, but we have not validated this in house. I hope this helps, please let me know if you need any additional information.

Read More
Application
Western blot
Sample
Human Cell lysate - other (Precipitated protein cell culture medium)
Loading amount
50 µg
Specification
Precipitated protein cell culture medium
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Apr 16 2010

Application
Western blot
Sample
Mouse Tissue lysate - whole (muscle)
Loading amount
3 µg
Specification
muscle
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% Tris Glycin)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Jan 14 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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